##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545455_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1665491
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86649582615577	31.0	31.0	33.0	28.0	34.0
2	31.154514194312668	31.0	31.0	34.0	28.0	34.0
3	31.22911561815705	31.0	31.0	34.0	28.0	34.0
4	35.099967517086554	35.0	35.0	37.0	32.0	37.0
5	34.98112928860018	35.0	35.0	37.0	32.0	37.0
6	35.01812558578821	37.0	35.0	37.0	32.0	37.0
7	35.53973272746595	37.0	35.0	37.0	35.0	37.0
8	35.44994238936145	37.0	35.0	37.0	35.0	37.0
9	37.13110968477164	39.0	37.0	39.0	34.0	39.0
10	36.45450380698545	38.0	35.0	39.0	32.0	39.0
11	36.212246118411926	38.0	35.0	39.0	31.0	39.0
12	35.77012604691349	37.0	35.0	39.0	30.0	39.0
13	35.46610158806022	37.0	35.0	39.0	30.0	39.0
14	36.497237751509914	38.0	35.0	40.0	30.0	41.0
15	36.719743907352246	38.0	35.0	40.0	31.0	41.0
16	36.71431247602059	38.0	35.0	40.0	31.0	41.0
17	36.62048368919436	38.0	35.0	40.0	31.0	41.0
18	36.50693399123742	38.0	35.0	40.0	30.0	41.0
19	36.43820290833154	38.0	35.0	40.0	30.0	41.0
20	36.30044713540932	38.0	35.0	40.0	30.0	41.0
21	36.1582722452418	38.0	34.0	40.0	30.0	41.0
22	36.05493395040862	38.0	34.0	40.0	30.0	41.0
23	35.96006042662494	38.0	34.0	40.0	30.0	41.0
24	35.84046506405618	37.0	34.0	40.0	30.0	41.0
25	35.68529220512149	37.0	34.0	40.0	29.0	41.0
26	35.531126256461306	37.0	34.0	40.0	29.0	41.0
27	35.452182569584586	37.0	34.0	40.0	28.0	41.0
28	35.436013764109205	37.0	34.0	40.0	28.0	41.0
29	35.42377473069503	37.0	34.0	40.0	28.0	41.0
30	35.259999603720466	37.0	34.0	40.0	27.0	41.0
31	35.10600957915714	37.0	34.0	40.0	27.0	41.0
32	34.887371952175066	37.0	33.0	40.0	25.0	41.0
33	34.60810835963689	37.0	33.0	40.0	24.0	41.0
34	34.3671367782834	37.0	33.0	40.0	23.0	41.0
35	34.16305041576328	37.0	33.0	40.0	23.0	41.0
36	33.99949864634513	37.0	33.0	40.0	22.0	41.0
37	33.9474857564526	37.0	33.0	40.0	22.0	41.0
38	33.88151902351919	36.0	33.0	40.0	22.0	41.0
39	33.79039394388802	36.0	33.0	40.0	21.0	41.0
40	33.633067966143315	36.0	32.0	40.0	21.0	41.0
41	33.5336384285475	36.0	32.0	40.0	20.0	41.0
42	33.487515693570245	36.0	32.0	40.0	20.0	41.0
43	33.39256111260883	36.0	32.0	40.0	20.0	41.0
44	33.29724387583001	35.0	32.0	40.0	20.0	41.0
45	33.24312409973996	35.0	32.0	40.0	20.0	41.0
46	33.190163741503255	35.0	32.0	40.0	20.0	41.0
47	33.07625859281137	35.0	31.0	39.0	20.0	41.0
48	33.0062173857439	35.0	31.0	39.0	20.0	41.0
49	33.00890968489172	35.0	31.0	39.0	20.0	41.0
50	32.93564060087986	35.0	31.0	39.0	20.0	41.0
51	32.75311965060153	35.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	4.0
13	11.0
14	28.0
15	82.0
16	256.0
17	704.0
18	1506.0
19	2754.0
20	4846.0
21	7326.0
22	11007.0
23	15297.0
24	20859.0
25	28766.0
26	36553.0
27	41823.0
28	44445.0
29	48530.0
30	55782.0
31	66119.0
32	78851.0
33	98086.0
34	140284.0
35	170533.0
36	139161.0
37	167372.0
38	217278.0
39	267161.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.62822675115026	21.515697172785682	26.034364640817632	17.821711435246424
2	34.536782246196466	24.32916179072718	27.966107292083837	13.16794867099252
3	28.302284431437936	24.781340757770533	33.332512754497024	13.58386205629451
4	25.738596005622366	27.690933184268186	31.63841773987371	14.932053070235742
5	22.72434975631811	31.05996970262823	31.650486253002867	14.565194288050792
6	21.044124525440246	39.34389318225076	29.699710175557836	9.912272116751156
7	83.60657607876595	4.34502498062133	10.129925649553195	1.9184732910595133
8	84.03882098432234	3.82866073728408	10.162288478292588	1.970229800100991
9	79.18907997701578	5.3938448181347125	11.95197092028717	3.46510428456233
10	40.84723363860868	25.228956505919275	19.717548758894523	14.206261096577528
11	34.205408495152476	25.25609564987142	25.446910250490696	15.091585604485406
12	31.057628050827052	22.412669897345587	30.394700421677452	16.135001630149908
13	23.18793677059798	28.72258090857291	30.438891594130503	17.650590726698614
14	19.631448023435734	31.02982844098227	31.893957997971768	17.44476553761023
15	19.20983061451548	23.407991997555076	40.08733760794865	17.294839779980798
16	21.343675828929726	21.38306361307266	38.60218998481529	18.671070573182323
17	21.731609477325307	21.5090324715054	31.070176902787228	25.68918114838207
18	23.431528600274635	23.181272069317696	33.81459281377083	19.57260651663684
19	28.01342066693846	23.918832344335694	28.6380412743149	19.429705714410943
20	29.27292912420421	23.10447789870975	28.924803556428703	18.697789420657333
21	25.28359504794682	25.186926858205776	30.405688172436836	19.123789921410562
22	24.29619853844902	23.910906753624005	29.647053031208216	22.14584167671876
23	21.999818672091294	26.3879540627959	29.46026126829866	22.151965996814152
24	21.71623863473294	23.79898780599835	35.485271310382345	18.999502248886365
25	21.84136690021141	24.702024808299775	33.091863000160316	20.364745291328504
26	20.612660170484258	27.83107203821576	30.14318300128911	21.41308479001087
27	20.230550630414694	27.974213009857152	31.07570079934386	20.719535560384294
28	19.523611955873672	26.92959613711512	33.9626572584301	19.58413464858111
29	20.72367848280177	24.86419920612	33.4884427475141	20.92367956356414
30	21.1687724520877	26.91800796281697	32.5496805446562	19.363539040439125
31	25.491821931190263	25.808785517303907	29.00646115770064	19.69293139380519
32	27.056465630856007	25.351322823119425	28.657915293448	18.93429625257657
33	26.399902491217304	26.392757451106007	27.49585557652368	19.711484481153004
34	21.961931946795268	26.87874026338179	29.413488274628925	21.745839515194017
35	22.108555374961497	27.378833028818526	29.853358559127606	20.659253037092366
36	26.007405623927117	27.448662286376813	27.017918439667344	19.52601365002873
37	22.561815104374624	30.37392576723621	27.891774857984824	19.172484270404343
38	22.820657691935892	29.406343234517628	26.94706846209316	20.82593061145332
39	22.48363995962752	29.43870606325702	27.019899837345264	21.057754139770193
40	24.32345776710892	26.039107986773868	27.810597595543896	21.826836650573313
41	20.359641691249006	26.538720413379597	28.61354399393332	24.488093901438074
42	22.35280767053079	27.006750561846328	27.230468372389883	23.409973395232996
43	21.882555954970638	26.895612164821063	28.533387451508297	22.688444428700006
44	21.72266316659772	27.546951619672516	29.163231743671986	21.56715347005778
45	20.364024783081984	30.79398207495567	26.84349540165633	21.99849774030601
46	22.06838704021817	29.420393145324713	27.712428346955942	20.798791467501175
47	21.760189637770484	28.577518581607464	28.172713031772613	21.48957874884944
48	22.402822951309854	27.25550603395635	29.569298182938248	20.77237283179555
49	21.841246815503656	27.046258430697012	30.03774862788211	21.074746125917223
50	20.887594108884407	30.04255201619222	28.364968648884926	20.704885226038446
51	20.33058119197282	30.569183502042343	27.423924836579722	21.67631046940512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1104.0
1	1890.5
2	2677.0
3	14336.0
4	25995.0
5	18776.0
6	11557.0
7	11648.5
8	11740.0
9	12073.0
10	12406.0
11	12457.5
12	12509.0
13	12385.0
14	12261.0
15	11825.0
16	11389.0
17	10842.0
18	10295.0
19	9932.0
20	9569.0
21	9477.5
22	9386.0
23	9539.0
24	9692.0
25	11066.0
26	14280.0
27	16120.0
28	18673.0
29	21226.0
30	23778.5
31	26331.0
32	31927.5
33	37524.0
34	42930.5
35	48337.0
36	52394.0
37	56451.0
38	60404.5
39	64358.0
40	76016.5
41	87675.0
42	99206.0
43	110737.0
44	113037.0
45	115337.0
46	117380.5
47	119424.0
48	115109.0
49	110794.0
50	110609.0
51	110424.0
52	107339.0
53	104254.0
54	98449.5
55	92645.0
56	90442.5
57	88240.0
58	83565.5
59	78891.0
60	74270.5
61	69650.0
62	61074.0
63	52498.0
64	44812.5
65	37127.0
66	30654.0
67	24181.0
68	19786.5
69	15392.0
70	12680.5
71	9969.0
72	8419.0
73	6869.0
74	5495.5
75	3060.0
76	1998.0
77	1469.0
78	940.0
79	725.0
80	510.0
81	396.0
82	282.0
83	197.0
84	112.0
85	66.5
86	21.0
87	19.0
88	17.0
89	11.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	3.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1665491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.862757469107052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78983703267261	17.556274201702486
2	5.771185477323644	2.6389042775456653
3	2.544702664499128	1.7453675954830217
4	1.6457937008311374	1.505095289051457
5	1.1192991079632997	1.2795132020376396
6	0.9015009260867848	1.2366478218778538
7	0.7664877549131673	1.2266816550534076
8	0.6184898396613924	1.1312306561028256
9	0.5683922837023884	1.1695513436639633
>10	7.48999000112161	40.55961479036392
>50	1.6739651769762418	25.290447225584867
>100	0.1085035349013711	3.400904972249339
>500	0.0010585710555739002	0.17389031601368854
>1k	5.292855277869501E-4	0.22616631915767846
>5k	0.0	0.0
>10k+	2.6464276389347505E-4	0.8597103341122132
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14209	0.8531418062301147	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2224	0.13353419502116795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00840592954269942	0.0
2	0.0	0.0	0.0	0.030501515769223612	0.0
3	0.0	0.0	0.0	0.04773367133175742	0.0
4	0.0	0.0	0.0	0.0673675210493482	0.0
5	0.0	0.0	0.0	0.11119843937913805	0.0
6	0.0	0.0	0.0	0.19825985249995345	0.0
7	0.0	0.0	0.0	0.25283835217362327	0.0
8	0.0	0.0	0.0	0.39243682493631005	0.0
9	0.0	0.0	0.0	0.465568411957795	0.0
10	0.0	0.0	0.0	0.5762865125059217	0.0
11	0.0	0.0	0.0	0.6565030972848247	0.0
12	0.0	0.0	0.0	0.7208084582864753	0.0
13	0.0	0.0	0.0	0.7571340823817121	0.0
14	0.0	0.0	0.0	0.7765878050376736	0.0
15	0.0	0.0	0.0	0.7953810618009944	0.0
16	0.0	0.0	0.0	0.8316466435423547	0.0
17	0.0	0.0	0.0	0.873255994778717	0.0
18	0.0	0.0	0.0	0.9313769933310958	0.0
19	0.0	0.0	0.0	0.9597770267146445	0.0
20	6.004235387642443E-5	0.0	0.0	0.9918996860385316	0.0
21	6.004235387642443E-5	0.0	0.0	1.0302067078116903	0.0
22	6.004235387642443E-5	0.0	0.0	1.073437202602716	0.0
23	6.004235387642443E-5	0.0	0.0	1.1224317633658782	0.0
24	6.004235387642443E-5	0.0	0.0	1.1606187004312842	0.0
25	6.004235387642443E-5	0.0	0.0	1.194242418602082	0.0
26	1.2008470775284887E-4	0.0	0.0	1.2276860097112503	0.0
27	1.2008470775284887E-4	0.0	0.0	1.2646720996991279	0.0
28	1.2008470775284887E-4	0.0	0.0	1.3055609426889727	0.0
29	1.2008470775284887E-4	0.0	0.0	1.3529343598974717	0.0
30	1.2008470775284887E-4	0.0	0.0	1.4144777726208067	0.0
31	1.8012706162927328E-4	0.0	0.0	1.465153519292509	0.0
32	1.8012706162927328E-4	0.0	0.0	1.515228842425447	0.0
33	1.8012706162927328E-4	0.0	0.0	1.5636830220037214	0.0
34	1.8012706162927328E-4	0.0	0.0	1.6189820299239084	0.0
35	1.8012706162927328E-4	0.0	0.0	1.6831072638639297	0.0
36	1.8012706162927328E-4	0.0	0.0	1.7439301683407475	0.0
37	2.4016941550569773E-4	0.0	0.0	1.804272733986554	0.0
38	3.0021176938212213E-4	0.0	0.0	1.8750626692068586	0.0
39	3.0021176938212213E-4	0.0	0.0	1.984339753261951	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	75	0.0	42.0	12
CGTTTTT	10945	0.0	41.751938	1
TCGACGG	70	0.0	38.57143	2
GCCCTAC	1185	0.0	38.16456	26
GGGCGAT	3835	0.0	37.842247	7
CGAGGGC	400	0.0	37.6875	4
ACGCATT	30	1.1401421E-4	37.499996	13
TATACTA	1175	0.0	37.148937	44
AGGGCGA	1945	0.0	36.902313	6
GGCGATT	1075	0.0	36.627907	8
AAGGGCG	870	0.0	36.206898	5
TTTACGG	175	0.0	36.0	2
TAACGCG	50	4.881258E-8	36.0	1
TCGGTAA	25	0.00210738	36.0	15
TCGTCCC	470	0.0	35.904255	38
TATGGGC	640	0.0	35.859375	4
TGGGCGA	1135	0.0	35.68282	6
ACCCACC	385	0.0	35.649353	13
GCGTAAG	120	0.0	35.625	1
TACGGGA	405	0.0	35.555553	4
>>END_MODULE