FastQCFastQC Report
Thu 26 May 2016
SRR1545454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545454_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1054068
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53740.5098342801413192No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44480.4219841604146981No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13120.12447014803599009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG351.2117926E-745.0000041
CGGAACG207.0330186E-445.00000434
GCGTTAG800.045.0000041
ACGGGTA453.8562575E-1045.0000045
TAGCACG207.0330186E-445.0000041
GTTCGCC351.2117926E-745.00000410
TGCGAAT207.0330186E-445.00000443
CGTGTAC351.2117926E-745.00000418
GTATACG453.8562575E-1045.0000041
CGACTTA453.8562575E-1045.00000433
TAGTCGT207.0330186E-445.00000438
TACGTGA406.8139343E-945.00000427
ACGCGAT253.8905848E-545.022
AACGTTG253.8905848E-545.01
CAAGTAC551.8189894E-1245.026
CGCGATT253.8905848E-545.023
CCGCTTG253.8905848E-545.039
TCCTATC253.8905848E-545.022
ATCACGC253.8905848E-545.033
ACGGGAT1950.045.05