##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545454_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1054068
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.950780215318176	31.0	31.0	33.0	30.0	34.0
2	31.226319364595074	31.0	31.0	34.0	28.0	34.0
3	31.335048592690416	31.0	31.0	34.0	28.0	34.0
4	35.16250564479711	35.0	35.0	37.0	32.0	37.0
5	35.03044964840978	37.0	35.0	37.0	32.0	37.0
6	35.0902958822391	37.0	35.0	37.0	32.0	37.0
7	35.56418466360804	37.0	35.0	37.0	35.0	37.0
8	35.48057905182588	37.0	35.0	37.0	35.0	37.0
9	37.19491816467249	39.0	37.0	39.0	34.0	39.0
10	36.63594189369187	39.0	35.0	39.0	32.0	39.0
11	36.292157621709414	38.0	35.0	39.0	32.0	39.0
12	35.22624251945795	37.0	35.0	39.0	30.0	39.0
13	34.62206233373938	37.0	33.0	39.0	27.0	39.0
14	35.55161716321907	37.0	33.0	40.0	27.0	41.0
15	36.00030263702152	37.0	34.0	40.0	30.0	41.0
16	36.1588094885719	37.0	34.0	40.0	31.0	41.0
17	36.111572498169	37.0	34.0	40.0	31.0	41.0
18	36.06351677500882	37.0	35.0	40.0	31.0	41.0
19	35.95112649278794	37.0	34.0	40.0	30.0	41.0
20	35.777526687082805	36.0	34.0	40.0	30.0	41.0
21	35.589239024427265	36.0	34.0	40.0	30.0	41.0
22	35.45867534162881	35.0	34.0	40.0	30.0	41.0
23	35.43716534417134	35.0	34.0	40.0	30.0	41.0
24	35.31199315414186	35.0	34.0	40.0	29.0	41.0
25	35.186195767256	35.0	34.0	40.0	29.0	41.0
26	35.03110804995503	35.0	34.0	40.0	29.0	41.0
27	34.914234186029745	35.0	34.0	40.0	29.0	41.0
28	34.999748593069896	36.0	34.0	40.0	29.0	41.0
29	35.10688779092051	36.0	34.0	40.0	29.0	41.0
30	35.03030070166251	36.0	34.0	40.0	29.0	41.0
31	34.83646311243677	35.0	34.0	40.0	28.0	41.0
32	34.544366207872734	35.0	33.0	40.0	27.0	41.0
33	34.29825400258807	35.0	33.0	40.0	25.0	41.0
34	34.1371088013297	35.0	33.0	40.0	24.0	41.0
35	33.967818964241395	35.0	33.0	40.0	23.0	41.0
36	33.7242938785733	35.0	33.0	40.0	23.0	41.0
37	33.65548996838913	35.0	33.0	40.0	23.0	41.0
38	33.67997415726499	35.0	33.0	40.0	23.0	41.0
39	33.644408140651265	35.0	33.0	40.0	23.0	41.0
40	33.437654876155996	35.0	32.0	39.0	23.0	41.0
41	33.442755116368204	35.0	32.0	39.0	23.0	41.0
42	33.40848977485324	35.0	32.0	39.0	23.0	41.0
43	33.28323694486504	35.0	32.0	39.0	23.0	41.0
44	33.229501322495324	35.0	32.0	39.0	23.0	41.0
45	33.180125950128456	35.0	32.0	39.0	23.0	41.0
46	33.14705123388624	35.0	32.0	39.0	23.0	41.0
47	33.070033432378175	35.0	32.0	39.0	23.0	41.0
48	33.05372328919956	35.0	32.0	39.0	23.0	40.0
49	33.08992588713441	35.0	32.0	39.0	22.0	40.0
50	32.94871583237514	35.0	32.0	39.0	22.0	40.0
51	32.764556935605675	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	8.0
14	12.0
15	53.0
16	146.0
17	363.0
18	812.0
19	1606.0
20	2818.0
21	4303.0
22	6282.0
23	9023.0
24	12689.0
25	17188.0
26	22295.0
27	25880.0
28	28193.0
29	31151.0
30	36368.0
31	44276.0
32	54516.0
33	69347.0
34	117482.0
35	157550.0
36	70981.0
37	82870.0
38	109843.0
39	147938.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.513113005992025	23.071851151918093	27.148153629557108	16.266882212532778
2	30.963941605285427	25.946713115282883	29.680722685822925	13.408622593608763
3	29.090058705889945	24.715293510475604	32.49135729383683	13.703290489797624
4	26.13616958298706	28.031113742187415	31.124367687853155	14.708348986972378
5	22.84672336130117	31.637807048501614	31.00511541949855	14.510354170698664
6	21.67858240644816	39.87731341810965	28.238880224046266	10.205223951395926
7	84.5306944144021	5.450976597335276	8.247380624399945	1.7709483638626728
8	86.33266544473412	4.053913030278881	7.809837695480747	1.8035838295062556
9	81.699093417123	5.5748775221332965	9.820334171988904	2.9056948887548053
10	54.594580235810206	19.90640072556989	14.6265705817841	10.872448456835802
11	49.85503781539711	19.850332236629896	18.457348102778948	11.837281845194047
12	44.30530098627413	20.670582922543897	23.748847322943114	11.275268768238861
13	20.722097625580133	43.089819632129995	23.401431406702415	12.786651335587457
14	14.565094472083395	45.31614658636825	27.2005221674503	12.918236774098066
15	13.835065669387554	22.09705635689538	51.470967717452766	12.596910256264302
16	15.189342623056575	17.01199543103481	50.17769252078613	17.620969425122478
17	15.786268058607225	17.380472607080378	29.527317023190154	37.305942311122244
18	21.586747724055755	21.83673159606401	36.54546006519504	20.0310606146852
19	31.22625864744969	21.62184982373054	27.26664693359442	19.885244595225355
20	32.74950003225599	20.978817305904364	25.97621785311763	20.29546480872202
21	22.244485175529473	27.48788503208522	30.001669721498043	20.265960070887267
22	23.366708789186276	24.680476022419807	25.306147231487913	26.646667956906008
23	19.647688763912765	31.131388107788112	25.334608393386386	23.886314734912737
24	19.007881844435083	22.820539092354572	41.93856563333675	16.233013429873594
25	17.075274080989082	24.062773938683275	39.262836932721605	19.599115047606038
26	15.929427702956545	34.893099875909336	29.296971352891845	19.880501068242275
27	18.04987913493247	34.82507769897198	29.056854017008387	18.068189149087154
28	14.508456759905433	29.10296109928392	40.08327735971493	16.305304781095717
29	14.827980737485627	24.452122633454387	39.74819461363024	20.971702015429745
30	16.773870376484247	31.35680051002402	33.31739508267019	18.551934030821542
31	29.513560794939224	26.305703237362295	25.774523085797117	18.406212881901357
32	31.54189293290376	25.951646383345288	27.35231503090882	15.154145652842132
33	28.981242196898112	27.28410311289215	25.270191296956174	18.464463393253567
34	19.615717392046815	27.12785133406953	29.184075410694565	24.07235586318909
35	20.614419563064242	24.82154851489657	31.570638706421217	22.993393215617967
36	31.8593297586114	24.79100020112554	25.011479335299054	18.338190704964006
37	20.32762592166729	34.538663539733676	28.166209390665497	16.967501147933532
38	20.77124056512483	35.39135995021194	23.439284752027383	20.398114732635843
39	20.934892246041052	33.16066895114926	26.481023994656894	19.423414808152796
40	24.733983006788936	26.301718674696513	25.403199793561704	23.56109852495285
41	17.30780177369961	25.384225685629392	26.49003669592474	30.81793584474626
42	21.64670590512187	26.274870311972283	24.4636968392931	27.614726943612744
43	22.079315565978668	27.06466755465492	25.371892515473387	25.48412436389303
44	19.713434047898236	30.40951817150317	28.963880888140043	20.913166892458552
45	16.869215268844133	38.346861872289075	22.8473874550788	21.93653540378799
46	20.712041348376005	35.294781740836456	25.400353677371857	18.592823233415682
47	22.596265136594603	27.579909455556944	26.78508407427225	23.0387413335762
48	23.360542204108274	24.148916388695984	31.27179650648725	21.218744900708494
49	21.038396004811837	25.09268851724936	33.44129600746821	20.427619470470596
50	19.4021638072686	35.15228619026477	26.492598200495603	18.95295180197103
51	18.824781702888238	35.62692350019164	23.96809314010102	21.580201656819103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	595.0
1	1185.0
2	1775.0
3	5945.5
4	10116.0
5	7721.5
6	5327.0
7	5549.5
8	5772.0
9	6278.5
10	6785.0
11	6956.5
12	7128.0
13	6920.5
14	6713.0
15	6384.0
16	6055.0
17	5685.5
18	5316.0
19	4968.5
20	4621.0
21	4751.5
22	4882.0
23	4447.0
24	4012.0
25	5101.5
26	7317.0
27	8443.0
28	10316.0
29	12189.0
30	13764.0
31	15339.0
32	17295.0
33	19251.0
34	21211.0
35	23171.0
36	24294.5
37	25418.0
38	31356.0
39	37294.0
40	57652.0
41	78010.0
42	95498.5
43	112987.0
44	111705.5
45	110424.0
46	105114.0
47	99804.0
48	89325.5
49	78847.0
50	75740.0
51	72633.0
52	69361.0
53	66089.0
54	60367.5
55	54646.0
56	48809.5
57	42973.0
58	40202.5
59	37432.0
60	34240.5
61	31049.0
62	25991.0
63	20933.0
64	17814.0
65	14695.0
66	11111.0
67	7527.0
68	5775.0
69	4023.0
70	3037.5
71	2052.0
72	1824.0
73	1596.0
74	1239.5
75	709.5
76	536.0
77	409.0
78	282.0
79	209.5
80	137.0
81	98.5
82	60.0
83	47.0
84	34.0
85	21.0
86	8.0
87	5.5
88	3.0
89	3.0
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1054068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.825412316888826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32797788033541	14.675301509326818
2	5.265575195738498	1.8772209789924266
3	1.775330179976569	0.9493797737009637
4	0.9269922133641716	0.6609607367104696
5	0.5738725429658816	0.511475734785417
6	0.4539229241473413	0.4854817969808527
7	0.3869014575705349	0.4827674605040037
8	0.3103170761543869	0.442522386513867
9	0.26964280081736436	0.4325844692565223
>10	4.269246077386233	18.853085523684076
>50	2.174846644200251	28.303341957448698
>100	1.2600471125755965	30.791321372313813
>500	0.003196736860656904	0.37903293677622696
>1k	0.001598368430328452	0.6451442594640214
>5k	5.327894767761508E-4	0.51037910354183
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5374	0.5098342801413192	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4448	0.4219841604146981	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1312	0.12447014803599009	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009487053966157781	0.0
2	0.0	0.0	0.0	0.03510209967478379	0.0
3	0.0	0.0	0.0	0.04753014037045048	0.0
4	0.0	0.0	0.0	0.07760410144317065	0.0
5	0.0	0.0	0.0	0.12560859451192902	0.0
6	0.0	0.0	0.0	0.18651548097466197	0.0
7	0.0	0.0	0.0	0.22256628604606155	0.0
8	0.0	0.0	0.0	0.3261649153565045	0.0
9	0.0	0.0	0.0	0.3683823055059066	0.0
10	0.0	0.0	0.0	0.4354557770466422	0.0
11	0.0	0.0	0.0	0.4963626635093751	0.0
12	0.0	0.0	0.0	0.5485414603232429	0.0
13	0.0	0.0	0.0	0.5699822022867594	0.0
14	0.0	0.0	0.0	0.5782359392373168	0.0
15	0.0	0.0	0.0	0.5913280737106145	0.0
16	0.0	0.0	0.0	0.6236789277352125	0.0
17	0.0	0.0	0.0	0.65659900499778	0.0
18	0.0	0.0	0.0	0.7111495653031873	0.0
19	0.0	0.0	0.0	0.7326851778063654	0.0
20	0.0	0.0	0.0	0.7578258708166835	0.0
21	0.0	0.0	0.0	0.7927382294121442	0.0
22	0.0	0.0	0.0	0.827176235309297	0.0
23	0.0	0.0	0.0	0.8664526387291901	0.0
24	0.0	0.0	0.0	0.894818930088002	0.0
25	0.0	0.0	0.0	0.9172083774481343	0.0
26	0.0	0.0	0.0	0.9414952356014982	0.0
27	0.0	0.0	0.0	0.965592352675539	0.0
28	0.0	0.0	0.0	0.9914920100031497	0.0
29	0.0	0.0	0.0	1.0209967478379005	0.0
30	0.0	0.0	0.0	1.0601782807181321	0.0
31	0.0	0.0	0.0	1.0993598135983638	0.0
32	0.0	0.0	0.0	1.1366439356853637	0.0
33	0.0	0.0	0.0	1.1725998702171017	0.0
34	0.0	0.0	0.0	1.2142480371285345	0.0
35	0.0	0.0	0.0	1.2640550704508628	0.0
36	0.0	0.0	0.0	1.3106365054246976	0.0
37	0.0	0.0	0.0	1.3536128598913921	0.0
38	0.0	0.0	0.0	1.4005737770238733	0.0
39	0.0	0.0	0.0	1.4434552609509064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	35	1.2117926E-7	45.000004	1
CGGAACG	20	7.0330186E-4	45.000004	34
GCGTTAG	80	0.0	45.000004	1
ACGGGTA	45	3.8562575E-10	45.000004	5
TAGCACG	20	7.0330186E-4	45.000004	1
GTTCGCC	35	1.2117926E-7	45.000004	10
TGCGAAT	20	7.0330186E-4	45.000004	43
CGTGTAC	35	1.2117926E-7	45.000004	18
GTATACG	45	3.8562575E-10	45.000004	1
CGACTTA	45	3.8562575E-10	45.000004	33
TAGTCGT	20	7.0330186E-4	45.000004	38
TACGTGA	40	6.8139343E-9	45.000004	27
ACGCGAT	25	3.8905848E-5	45.0	22
AACGTTG	25	3.8905848E-5	45.0	1
CAAGTAC	55	1.8189894E-12	45.0	26
CGCGATT	25	3.8905848E-5	45.0	23
CCGCTTG	25	3.8905848E-5	45.0	39
TCCTATC	25	3.8905848E-5	45.0	22
ATCACGC	25	3.8905848E-5	45.0	33
ACGGGAT	195	0.0	45.0	5
>>END_MODULE