FastQCFastQC Report
Thu 26 May 2016
SRR1545453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545453_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1214960
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67420.5549153881609271No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47690.3925232106406796No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17850.14691841706722855No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16780.13811154276684004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG207.0334465E-445.019
GACACGT351.2120108E-745.039
CAATTCG502.1827873E-1145.01
ATAGACG351.2120108E-745.01
ACGGGTA1100.045.05
GTTACTA351.2120108E-745.09
ACGATAT207.0334465E-445.012
CCCGTAA207.0334465E-445.021
CCGTAAA207.0334465E-445.022
TCGCTAC207.0334465E-445.041
TCTCGAG253.8909384E-545.01
ACTACGT253.8909384E-545.019
ACTACGG453.8562575E-1045.02
CAATCGA253.8909384E-545.042
TATCGGA253.8909384E-545.013
AATACGG1100.045.02
TACGTCC207.0334465E-445.029
ATCGACG351.2120108E-745.029
TGCGTAA207.0334465E-445.034
ACTCTCG302.165605E-644.9999969
Produced by FastQC (version 0.11.5)