##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545453_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1214960
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.939049845262396	31.0	31.0	33.0	28.0	34.0
2	31.219516691907554	31.0	31.0	34.0	28.0	34.0
3	31.322255053664318	31.0	31.0	34.0	28.0	34.0
4	35.15594258247185	35.0	35.0	37.0	32.0	37.0
5	35.032661157568974	37.0	35.0	37.0	32.0	37.0
6	35.08656745901099	37.0	35.0	37.0	32.0	37.0
7	35.56991176664253	37.0	35.0	37.0	35.0	37.0
8	35.49135362481069	37.0	35.0	37.0	35.0	37.0
9	37.18988361756766	39.0	37.0	39.0	34.0	39.0
10	36.569647560413514	39.0	35.0	39.0	32.0	39.0
11	36.27012411931257	38.0	35.0	39.0	32.0	39.0
12	35.44288042404688	37.0	35.0	39.0	30.0	39.0
13	34.983562421808124	37.0	34.0	39.0	29.0	39.0
14	35.947971949693816	38.0	34.0	40.0	29.0	41.0
15	36.285003621518406	38.0	35.0	40.0	30.0	41.0
16	36.40499193389083	38.0	35.0	40.0	31.0	41.0
17	36.34642539672088	38.0	35.0	40.0	31.0	41.0
18	36.30764634226641	38.0	35.0	40.0	31.0	41.0
19	36.20943158622506	37.0	35.0	40.0	31.0	41.0
20	36.043760288404556	37.0	34.0	40.0	30.0	41.0
21	35.88489415289392	37.0	34.0	40.0	30.0	41.0
22	35.78007753341674	37.0	34.0	40.0	30.0	41.0
23	35.70715990649898	36.0	34.0	40.0	30.0	41.0
24	35.590846612234145	36.0	34.0	40.0	30.0	41.0
25	35.468634358332785	36.0	34.0	40.0	29.0	41.0
26	35.329717027721074	36.0	34.0	40.0	29.0	41.0
27	35.2260485941924	36.0	34.0	40.0	29.0	41.0
28	35.26798742345427	36.0	34.0	40.0	29.0	41.0
29	35.3384350102061	36.0	34.0	40.0	29.0	41.0
30	35.23344883782182	36.0	34.0	40.0	29.0	41.0
31	35.069695298610654	36.0	34.0	40.0	28.0	41.0
32	34.85367007967341	36.0	34.0	40.0	27.0	41.0
33	34.60321738987292	36.0	33.0	40.0	25.0	41.0
34	34.4384975637058	36.0	33.0	40.0	24.0	41.0
35	34.26099542371765	36.0	33.0	40.0	24.0	41.0
36	34.052663462171594	35.0	33.0	40.0	23.0	41.0
37	33.99641716599724	35.0	33.0	40.0	23.0	41.0
38	34.01588447356291	35.0	33.0	40.0	23.0	41.0
39	33.96891091064727	35.0	33.0	40.0	23.0	41.0
40	33.80873526700468	35.0	33.0	40.0	23.0	41.0
41	33.7827023111872	35.0	33.0	40.0	23.0	41.0
42	33.70806363995523	35.0	33.0	40.0	23.0	41.0
43	33.6195619608876	35.0	33.0	40.0	23.0	41.0
44	33.53724978600118	35.0	32.0	40.0	23.0	41.0
45	33.505802660169884	35.0	32.0	40.0	23.0	41.0
46	33.421148021334034	35.0	32.0	39.0	23.0	41.0
47	33.357568150391785	35.0	32.0	39.0	23.0	41.0
48	33.344636037400406	35.0	32.0	39.0	23.0	41.0
49	33.373976097978534	35.0	32.0	39.0	23.0	41.0
50	33.28866300125107	35.0	32.0	39.0	22.0	41.0
51	33.112640745374335	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	10.0
14	23.0
15	61.0
16	153.0
17	416.0
18	863.0
19	1701.0
20	3093.0
21	4691.0
22	7106.0
23	9804.0
24	13713.0
25	18765.0
26	24532.0
27	28785.0
28	30745.0
29	34335.0
30	40127.0
31	48645.0
32	60204.0
33	76496.0
34	123227.0
35	163934.0
36	88741.0
37	102480.0
38	138934.0
39	193268.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58617567656548	24.19758675182722	27.175544873905316	16.04069269770198
2	31.37272008955027	26.197981826562188	29.56829854480806	12.860999539079476
3	28.658885889247383	25.444129847896228	32.08377230526108	13.813211957595312
4	26.3588924738263	28.034750115230132	30.750230460262067	14.856126950681503
5	22.690705866859815	32.80264370843485	30.166672153815767	14.339978270889578
6	20.664877856061107	40.34774807401067	28.601023902021467	10.386350167906762
7	84.52607493250807	5.326018963587279	8.253028906301441	1.8948771976032133
8	86.03484888391387	4.331665240007902	7.8121090406268525	1.8213768354513729
9	81.29839665503391	5.906284980575492	9.768716665569237	3.0266016988213607
10	48.76720221241852	24.42607164021861	15.745456640547836	11.061269506815039
11	43.5101567129782	21.151313623493778	21.97562059656285	13.362909066965168
12	40.17835978139198	21.85265358530322	25.3804240468822	12.588562586422597
13	21.829607559096594	39.7396622111016	23.909182195298612	14.521548034503192
14	15.722904457759926	40.80233094093633	27.70371041021927	15.77105419108448
15	14.45380917890301	23.68398959636531	46.81734378086521	15.044857443866464
16	15.202228879963126	19.150424705340093	45.52923553038783	20.118110884308948
17	16.286873641930598	19.490929742542964	31.068265621913476	33.15393099361296
18	20.87739514058076	23.1516263909923	35.29647066570093	20.674507802726016
19	29.34969052479094	24.0232600250214	26.11295845130704	20.514090998880622
20	30.23720945545532	22.68387436623428	27.26476591821953	19.81415026009087
21	21.875781918746295	28.147511029169685	29.01305392770132	20.963653124382695
22	22.73367024428788	26.006699809047213	25.546026206624084	25.713603740040824
23	19.806001843682097	29.929709620069794	26.675199183512216	23.589089352735893
24	18.879469282939358	23.396325804964775	40.66619477184434	17.058010140251533
25	17.134144333969843	26.373625469151246	35.873444393231054	20.618785803647857
26	16.912984789622705	34.00441166787384	28.35829986172384	20.724303680779617
27	17.814084414301707	34.362365839204585	29.122851781128595	18.700697965365116
28	15.231283334430762	28.885724632909728	37.939191413709096	17.943800618950416
29	16.155182063607032	24.223184302363865	37.34509778099691	22.276535853032197
30	17.63366695199842	32.35143543820373	31.949035359188784	18.065862250609076
31	27.312339500888918	27.626094686244812	26.66063080266017	18.400935010206098
32	28.878234674392573	26.598818068084544	27.24377757292421	17.27916968459867
33	27.265671297820504	28.291219464015278	24.791515770066503	19.651593468097715
34	18.422664120629488	27.79795219595707	29.72418845064858	24.055195232764863
35	20.479439652334232	26.36226707052084	31.254197669059064	21.904095608085864
36	28.31352472509383	27.980180417462304	24.957941002172912	18.748353855270956
37	19.200220583393694	35.35927108711398	28.15170869822875	17.28879963126358
38	19.834973990913284	33.409001119378416	25.724879831434777	21.031145058273523
39	19.848472377691447	33.57246329097254	26.99660894185817	19.582455389477843
40	23.139115691051558	28.037137025087244	26.214937117271354	22.608810166589848
41	17.307154144992428	25.556067689471256	28.52414894317508	28.612629222361228
42	20.28758148416409	27.050767103443736	25.300092184104827	27.36155922828735
43	20.799697109369855	27.51316915783236	26.42432672680582	25.262807005991966
44	19.205488246526635	30.522157108052937	28.77074142358596	21.501613221834464
45	17.167890300915257	37.5847764535458	23.238789754395206	22.008543491143744
46	19.49298742345427	35.39474550602489	25.83928689010338	19.272980180417463
47	21.00883979719497	28.6557582142622	27.620003950747353	22.715398037795484
48	20.900441166787385	25.132679265160995	32.08788766708369	21.878991900967932
49	20.112925528412458	26.622934088365046	32.62477777046158	20.639362612760912
50	18.830825706196087	34.34327056034767	27.948162902482387	18.87774083097386
51	18.00446105221571	33.017218673865806	25.828833871073947	23.149486402844538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	635.0
1	1329.5
2	2024.0
3	7400.0
4	12776.0
5	9742.5
6	6709.0
7	6922.0
8	7135.0
9	7387.0
10	7639.0
11	7831.5
12	8024.0
13	7796.0
14	7568.0
15	7097.0
16	6626.0
17	6125.0
18	5624.0
19	5364.5
20	5105.0
21	5680.5
22	6256.0
23	5783.0
24	5310.0
25	6887.5
26	10051.5
27	11638.0
28	14452.0
29	17266.0
30	20125.5
31	22985.0
32	24606.0
33	26227.0
34	32504.5
35	38782.0
36	39652.0
37	40522.0
38	47541.5
39	54561.0
40	69433.5
41	84306.0
42	100594.0
43	116882.0
44	116724.0
45	116566.0
46	112050.0
47	107534.0
48	98958.0
49	90382.0
50	85332.5
51	80283.0
52	76246.5
53	72210.0
54	63485.5
55	54761.0
56	51915.5
57	49070.0
58	46465.0
59	43860.0
60	39042.5
61	34225.0
62	29903.0
63	25581.0
64	20449.0
65	15317.0
66	12240.0
67	9163.0
68	7377.5
69	5592.0
70	4326.5
71	3061.0
72	2408.0
73	1755.0
74	1431.0
75	875.5
76	644.0
77	513.5
78	383.0
79	293.5
80	204.0
81	160.5
82	117.0
83	87.0
84	57.0
85	38.5
86	20.0
87	10.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1214960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.44330734081381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91135450491069	14.92272978495608
2	5.719516836417647	2.1097361371001955
3	2.0067611139147776	1.1103393595057236
4	1.091562783830412	0.805281116159144
5	0.7705661839115806	0.710589447815967
6	0.52522134920775	0.5812091259237258
7	0.4250960336230011	0.5488123758228951
8	0.37559665562300015	0.5541795644669436
9	0.2985903860008675	0.49562948322236067
>10	4.685762794522685	21.010154363387517
>50	2.068843624120665	27.7940311907689
>100	1.1175429929611835	27.83832789160754
>500	8.961852389424087E-4	0.10503937880431405
>1k	0.002240463097356022	0.8567610071316474
>5k	4.4809261947120435E-4	0.5571797733270537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6742	0.5549153881609271	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4769	0.3925232106406796	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1785	0.14691841706722855	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1678	0.13811154276684004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010946862448146442	0.0
2	0.0	0.0	0.0	0.040248238625139925	0.0
3	0.0	0.0	0.0	0.05432277605847106	0.0
4	0.0	0.0	0.0	0.08197800750641997	0.0
5	0.0	0.0	0.0	0.13128004214130506	0.0
6	0.0	0.0	0.0	0.1972081385395404	0.0
7	0.0	0.0	0.0	0.23679791927306248	0.0
8	0.0	0.0	0.0	0.359600316059788	0.0
9	0.0	0.0	0.0	0.4156515440837558	0.0
10	0.0	0.0	0.0	0.501991835122144	0.0
11	0.0	0.0	0.0	0.5703891486139462	0.0
12	0.0	0.0	0.0	0.640103377888984	0.0
13	0.0	0.0	0.0	0.6675939948640285	0.0
14	0.0	0.0	0.0	0.6781293211299138	0.0
15	0.0	0.0	0.0	0.6930269309277671	0.0
16	0.0	0.0	0.0	0.7283367353657734	0.0
17	0.0	0.0	0.0	0.7701488114835057	0.0
18	0.0	0.0	0.0	0.8396984262856391	0.0
19	0.0	0.0	0.0	0.8639790610390465	0.0
20	0.0	0.0	0.0	0.8973134918021992	0.0
21	0.0	0.0	0.0	0.9304833080924475	0.0
22	0.0	0.0	0.0	0.9709784684269441	0.0
23	0.0	0.0	0.0	1.0192105089879502	0.0
24	0.0	0.0	0.0	1.05797721735695	0.0
25	0.0	0.0	0.0	1.089253967208797	0.0
26	8.230723645222887E-5	0.0	0.0	1.1216830183709752	0.0
27	8.230723645222887E-5	0.0	0.0	1.1523836175676565	0.0
28	8.230723645222887E-5	0.0	0.0	1.1818496082175545	0.0
29	8.230723645222887E-5	0.0	0.0	1.2195463225126753	0.0
30	8.230723645222887E-5	0.0	0.0	1.276996773556331	0.0
31	1.6461447290445775E-4	0.0	0.0	1.324817277935076	0.0
32	1.6461447290445775E-4	0.0	0.0	1.3732139329689865	0.0
33	1.6461447290445775E-4	0.0	0.0	1.4184829130177126	0.0
34	1.6461447290445775E-4	0.0	0.0	1.469760321327451	0.0
35	1.6461447290445775E-4	0.0	0.0	1.531573055903075	0.0
36	1.6461447290445775E-4	0.0	0.0	1.5782412589714887	0.0
37	2.4692170935668666E-4	0.0	0.0	1.6270494501876605	0.0
38	2.4692170935668666E-4	0.0	0.0	1.6793968525712781	0.0
39	2.4692170935668666E-4	0.0	0.0	1.7313327187726344	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	20	7.0334465E-4	45.0	19
GACACGT	35	1.2120108E-7	45.0	39
CAATTCG	50	2.1827873E-11	45.0	1
ATAGACG	35	1.2120108E-7	45.0	1
ACGGGTA	110	0.0	45.0	5
GTTACTA	35	1.2120108E-7	45.0	9
ACGATAT	20	7.0334465E-4	45.0	12
CCCGTAA	20	7.0334465E-4	45.0	21
CCGTAAA	20	7.0334465E-4	45.0	22
TCGCTAC	20	7.0334465E-4	45.0	41
TCTCGAG	25	3.8909384E-5	45.0	1
ACTACGT	25	3.8909384E-5	45.0	19
ACTACGG	45	3.8562575E-10	45.0	2
CAATCGA	25	3.8909384E-5	45.0	42
TATCGGA	25	3.8909384E-5	45.0	13
AATACGG	110	0.0	45.0	2
TACGTCC	20	7.0334465E-4	45.0	29
ATCGACG	35	1.2120108E-7	45.0	29
TGCGTAA	20	7.0334465E-4	45.0	34
ACTCTCG	30	2.165605E-6	44.999996	9
>>END_MODULE