FastQCFastQC Report
Thu 26 May 2016
SRR1545452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545452_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1227410
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68720.5598781173365053No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57220.46618489339340563No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17020.13866597143578754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG207.033473E-445.00000444
ATCTCGC406.8157533E-945.00000410
ATCTCGA207.033473E-445.00000417
GCGACAA406.8157533E-945.00000436
CGTTGCT207.033473E-445.00000445
CGGCACT207.033473E-445.00000422
CGGCACG207.033473E-445.00000426
ACCGAGT207.033473E-445.00000418
GTTAGTC207.033473E-445.00000441
TCGATTG207.033473E-445.0000041
TAACCGA207.033473E-445.00000416
CGCTAAC207.033473E-445.00000441
TCTAACG207.033473E-445.0000041
CCGAGTC207.033473E-445.00000419
GCGATAT950.045.0000049
CTCGACA207.033473E-445.00000429
TATAGCG551.8189894E-1245.0000041
CATCGAT207.033473E-445.00000434
CCGCGTG207.033473E-445.00000440
CGCCGAT207.033473E-445.00000411