##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545452_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1227410
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.957919521594253	31.0	31.0	33.0	28.0	34.0
2	31.249086287385634	31.0	31.0	34.0	28.0	34.0
3	31.324597322817965	31.0	31.0	34.0	28.0	34.0
4	35.141625862588704	35.0	35.0	37.0	32.0	37.0
5	35.00845846131285	35.0	35.0	37.0	32.0	37.0
6	35.051210272036236	37.0	35.0	37.0	32.0	37.0
7	35.59027138445996	37.0	35.0	37.0	35.0	37.0
8	35.54962074612395	37.0	35.0	37.0	35.0	37.0
9	37.273162187044264	39.0	37.0	39.0	34.0	39.0
10	36.65610757611556	39.0	35.0	39.0	32.0	39.0
11	36.25710072428936	38.0	35.0	39.0	32.0	39.0
12	34.978720232033304	35.0	34.0	39.0	30.0	39.0
13	34.25484801329629	35.0	33.0	39.0	25.0	39.0
14	35.116607327624834	36.0	33.0	40.0	25.0	41.0
15	35.669343577125815	36.0	33.0	40.0	30.0	41.0
16	35.903156239561355	36.0	34.0	40.0	31.0	41.0
17	35.85442191280827	36.0	34.0	40.0	31.0	41.0
18	35.8037672823262	36.0	34.0	40.0	30.0	41.0
19	35.66367310026804	36.0	34.0	40.0	30.0	41.0
20	35.47962864894371	35.0	34.0	40.0	30.0	41.0
21	35.269495930455186	35.0	34.0	40.0	29.0	41.0
22	35.10853423061569	35.0	34.0	40.0	29.0	41.0
23	35.1300413064909	35.0	34.0	40.0	29.0	41.0
24	35.011735279979796	35.0	34.0	39.0	29.0	41.0
25	34.89978735711783	35.0	34.0	39.0	29.0	41.0
26	34.76086556244449	35.0	34.0	39.0	29.0	41.0
27	34.6017997246234	35.0	33.0	39.0	27.0	41.0
28	34.68951369143155	35.0	34.0	39.0	28.0	41.0
29	34.810148198238565	35.0	34.0	39.0	29.0	41.0
30	34.72015463455569	35.0	34.0	39.0	29.0	41.0
31	34.48394179613984	35.0	33.0	39.0	27.0	41.0
32	34.186339527949094	35.0	33.0	39.0	25.0	41.0
33	33.89445091697151	35.0	33.0	39.0	24.0	41.0
34	33.73118680799407	35.0	33.0	39.0	23.0	41.0
35	33.51450452579008	35.0	33.0	39.0	23.0	41.0
36	33.24644821208887	35.0	33.0	39.0	21.0	41.0
37	33.18788098516388	35.0	32.0	39.0	22.0	41.0
38	33.220773009833714	35.0	32.0	39.0	23.0	41.0
39	33.188006452611596	35.0	32.0	39.0	22.0	41.0
40	33.0059515565296	35.0	32.0	39.0	21.0	41.0
41	33.02734131219397	35.0	32.0	39.0	21.0	41.0
42	32.99340807065284	35.0	32.0	39.0	22.0	41.0
43	32.84751875901288	35.0	32.0	38.0	21.0	41.0
44	32.79219168818895	35.0	32.0	38.0	21.0	41.0
45	32.750183720191295	35.0	32.0	38.0	21.0	40.0
46	32.69405251708883	35.0	32.0	38.0	21.0	40.0
47	32.622674574917916	35.0	32.0	38.0	20.0	40.0
48	32.628882769408754	35.0	32.0	38.0	20.0	40.0
49	32.6834505177569	35.0	32.0	38.0	20.0	40.0
50	32.549896122729976	35.0	32.0	38.0	20.0	40.0
51	32.34650279857586	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	6.0
14	16.0
15	55.0
16	192.0
17	482.0
18	1077.0
19	2168.0
20	3556.0
21	5675.0
22	8316.0
23	11567.0
24	16333.0
25	22106.0
26	28770.0
27	32886.0
28	35041.0
29	38100.0
30	44450.0
31	52973.0
32	65597.0
33	84408.0
34	145718.0
35	200483.0
36	76882.0
37	85238.0
38	112401.0
39	152842.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.661800050512866	22.858458053951004	29.469696352482057	17.010045543054073
2	31.37989750776024	26.3281218174856	29.026323722309577	13.26565695244458
3	28.13151269746865	25.07059580743191	33.24422971949063	13.553661775608802
4	24.362926813371246	28.054847198572602	32.40099070400274	15.181235284053413
5	22.548537163620956	31.51049771470006	32.12129606244042	13.81966905923856
6	20.74693867574812	40.131985237206806	29.047180648682996	10.07389543836208
7	84.15875705754394	5.280061267221222	8.911040320675243	1.6501413545596013
8	86.87243871241068	3.531175401862458	8.186425073936174	1.4099608117906812
9	82.98229605429319	4.900644446435991	9.63101164240148	2.4860478568693427
10	58.23946358592483	17.85523989538948	14.20658133793924	9.698715180746449
11	54.388835026600724	16.92580311387393	19.16702650296152	9.51833535656382
12	47.504012514155825	19.437922128710046	22.172216292844283	10.885849064289847
13	20.56191492655266	46.13796530906543	21.438802030291427	11.861317734090484
14	13.105889637529433	47.179345125100824	28.040019227479	11.674746009890745
15	12.52996146356963	20.954937632901803	54.58917558110167	11.925925322426899
16	13.36399410140051	15.373347129321091	52.876137557947224	18.38652121133118
17	13.856413097497983	16.030992089033006	30.959907447389217	39.15268736607979
18	21.144605307110094	20.231137109849197	38.21355537269535	20.41070221034536
19	32.00951597265787	20.929762670990133	27.194498985669007	19.86622237068298
20	34.0722334020417	20.57935001344294	26.37790143472841	18.970515149786948
21	22.2505927114819	28.778403304519273	29.051987518433126	19.919016465565704
22	22.368890590756145	24.360727059417798	26.133810218264475	27.136572131561582
23	18.470682168142673	31.557996105620777	25.340269347650747	24.631052378585803
24	17.861105905932003	22.7051270561589	44.41710593852095	15.016661099388143
25	15.141639712891372	23.353728582951092	41.664236074335385	19.840395629822147
26	14.30614057242486	36.51347145615564	29.408755020734716	19.771632950684776
27	16.17250959337141	36.829665718871446	29.959345288045558	17.038479399711587
28	13.365786493510726	28.996178945910493	43.04494830578209	14.593086254796686
29	13.361305513235186	23.891120326541255	42.48181129369974	20.26576286652382
30	16.748519239699856	32.256784611499015	33.91515467529187	17.07954147350926
31	30.938235797329334	25.812320251586673	26.14008359065023	17.109360360433758
32	32.81210027619133	24.8429620094345	27.837234501918672	14.507703212455494
33	30.39994785768407	26.568220887885875	25.112472604916043	17.919358649514017
34	18.680147627931987	27.109767722276988	28.90517431013272	25.304910339658303
35	19.45340187875282	24.698104137981602	33.680840143065474	22.167653840200096
36	32.968690168729275	23.853805981701306	26.087615385242096	17.089888464327323
37	19.820842261347064	34.42207575300837	29.320438973122265	16.436643012522303
38	19.814813306067247	35.83749521349834	23.54926226770191	20.798429212732504
39	20.065014950179645	33.76679349198719	27.12239593941715	19.045795618416015
40	24.19444195501096	26.904131463814046	24.78185773294987	24.119568848225125
41	16.553718806266854	23.498342037298052	27.792913533375156	32.15502562305994
42	21.27227250877865	24.065063833600835	24.340358967256254	30.322304690364266
43	21.217115715205185	25.73663242111438	26.01575675609617	27.03049510758426
44	18.207119055572303	31.635965162415168	29.465052427469224	20.691863354543308
45	16.084193545758957	40.66986581500884	22.69755012587481	20.548390513357397
46	21.212390317823708	35.70070310654142	25.091941568017205	17.994965007617665
47	21.811456644479023	27.29267318988765	26.750637521284652	24.145232644348667
48	23.280810813012767	23.171311949552308	33.46925640169137	20.078620835743557
49	21.624477558436055	22.8415118012726	34.65223519443381	20.881775445857535
50	18.912262406204935	36.091444586568464	26.361281071524594	18.635011935702007
51	17.730994533204065	36.269706129166295	23.667723091713444	22.3315762459162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	832.0
1	1388.5
2	1945.0
3	7022.5
4	12100.0
5	9354.0
6	6608.0
7	7119.5
8	7631.0
9	8435.0
10	9239.0
11	9505.0
12	9771.0
13	9142.5
14	8514.0
15	8096.0
16	7678.0
17	7253.5
18	6829.0
19	6439.0
20	6049.0
21	5636.0
22	5223.0
23	5267.5
24	5312.0
25	6273.5
26	7350.5
27	7466.0
28	9545.5
29	11625.0
30	13607.0
31	15589.0
32	17880.5
33	20172.0
34	22780.5
35	25389.0
36	26764.5
37	28140.0
38	36192.5
39	44245.0
40	68648.0
41	93051.0
42	117986.0
43	142921.0
44	146897.5
45	150874.0
46	137567.0
47	124260.0
48	109842.0
49	95424.0
50	87668.0
51	79912.0
52	75636.5
53	71361.0
54	62063.0
55	52765.0
56	48993.5
57	45222.0
58	43523.5
59	41825.0
60	34899.5
61	27974.0
62	25392.0
63	22810.0
64	18116.0
65	13422.0
66	10507.5
67	7593.0
68	5905.5
69	4218.0
70	3430.5
71	2643.0
72	2072.0
73	1501.0
74	1211.0
75	743.0
76	565.0
77	409.5
78	254.0
79	220.0
80	186.0
81	136.5
82	87.0
83	54.0
84	21.0
85	12.5
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1227410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.506723133887874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05306392081019	14.53986995482716
2	5.886468644648823	2.061055535963583
3	1.9028661801680953	0.9993885413112494
4	0.9196160245977186	0.6439785252847554
5	0.5668913009675679	0.49622045265243575
6	0.3978696867664159	0.41792366697518013
7	0.34638362619738433	0.4244829569364803
8	0.26449229450418343	0.37043146967451784
9	0.2166665656467282	0.3413809419432851
>10	3.2690070920482714	14.06814049651269
>50	1.5216790871504804	19.727891569620446
>100	1.6498500277825643	44.26590006377592
>500	0.0032744400891630138	0.38194639385916396
>1k	9.355543111894326E-4	0.23003589630154192
>5k	9.355543111894326E-4	1.031353534361559
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6872	0.5598781173365053	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5722	0.46618489339340563	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1702	0.13866597143578754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010021101343479359	0.0
2	0.0	0.0	0.0	0.03698845536536284	0.0
3	0.0	0.0	0.0	0.053690290937828435	0.0
4	0.0	0.0	0.0	0.0921452489388224	0.0
5	0.0	0.0	0.0	0.1443690372410197	0.0
6	0.0	0.0	0.0	0.22828557694657856	0.0
7	0.0	0.0	0.0	0.2762728020791749	0.0
8	0.0	0.0	0.0	0.42218981432447183	0.0
9	0.0	0.0	0.0	0.47922047237679344	0.0
10	0.0	0.0	0.0	0.5737284200063548	0.0
11	0.0	0.0	0.0	0.648845943898127	0.0
12	0.0	0.0	0.0	0.7253484980568841	0.0
13	8.147236864617365E-5	0.0	0.0	0.7572041941975379	0.0
14	8.147236864617365E-5	0.0	0.0	0.7696694666004025	0.0
15	8.147236864617365E-5	0.0	0.0	0.7871045534906836	0.0
16	8.147236864617365E-5	0.0	0.0	0.8260483457035547	0.0
17	8.147236864617365E-5	0.0	0.0	0.8683325050309187	0.0
18	8.147236864617365E-5	0.0	0.0	0.9412502749692442	0.0
19	8.147236864617365E-5	0.0	0.0	0.9652846237198655	0.0
20	8.147236864617365E-5	0.0	0.0	0.9957552895935343	0.0
21	8.147236864617365E-5	0.0	0.0	1.0352693883869286	0.0
22	8.147236864617365E-5	0.0	0.0	1.0718504819090606	0.0
23	8.147236864617365E-5	0.0	0.0	1.1212227373086419	0.0
24	8.147236864617365E-5	0.0	0.0	1.1530784334492956	0.0
25	8.147236864617365E-5	0.0	0.0	1.1817567072127488	0.0
26	8.147236864617365E-5	0.0	0.0	1.2142641823025722	0.0
27	8.147236864617365E-5	0.0	0.0	1.2445719034389486	0.0
28	1.629447372923473E-4	0.0	0.0	1.2740649008888636	0.0
29	1.629447372923473E-4	0.0	0.0	1.3064909036100407	0.0
30	1.629447372923473E-4	0.0	0.0	1.3567593550647299	0.0
31	1.629447372923473E-4	0.0	0.0	1.3993694038666786	0.0
32	1.629447372923473E-4	0.0	0.0	1.44043147766435	0.0
33	1.629447372923473E-4	0.0	0.0	1.4819823856738987	0.0
34	1.629447372923473E-4	0.0	0.0	1.525325685793663	0.0
35	1.629447372923473E-4	0.0	0.0	1.5916441938716483	0.0
36	1.629447372923473E-4	0.0	0.0	1.641179394008522	0.0
37	1.629447372923473E-4	0.0	0.0	1.6925069862556115	0.0
38	1.629447372923473E-4	0.0	0.0	1.739760960070392	0.0
39	1.629447372923473E-4	0.0	0.0	1.7900294115250812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	7.033473E-4	45.000004	44
ATCTCGC	40	6.8157533E-9	45.000004	10
ATCTCGA	20	7.033473E-4	45.000004	17
GCGACAA	40	6.8157533E-9	45.000004	36
CGTTGCT	20	7.033473E-4	45.000004	45
CGGCACT	20	7.033473E-4	45.000004	22
CGGCACG	20	7.033473E-4	45.000004	26
ACCGAGT	20	7.033473E-4	45.000004	18
GTTAGTC	20	7.033473E-4	45.000004	41
TCGATTG	20	7.033473E-4	45.000004	1
TAACCGA	20	7.033473E-4	45.000004	16
CGCTAAC	20	7.033473E-4	45.000004	41
TCTAACG	20	7.033473E-4	45.000004	1
CCGAGTC	20	7.033473E-4	45.000004	19
GCGATAT	95	0.0	45.000004	9
CTCGACA	20	7.033473E-4	45.000004	29
TATAGCG	55	1.8189894E-12	45.000004	1
CATCGAT	20	7.033473E-4	45.000004	34
CCGCGTG	20	7.033473E-4	45.000004	40
CGCCGAT	20	7.033473E-4	45.000004	11
>>END_MODULE