FastQCFastQC Report
Thu 26 May 2016
SRR1545451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545451_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1971196
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99560.5050740768548637No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61510.31204405853096295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG253.891824E-545.0000041
CTAACCG253.891824E-545.0000041
ACCTACG253.891824E-545.00000429
CTACGCG253.891824E-545.0000041
CGACGAT207.034516E-445.014
TAGCACG302.1662945E-644.9999961
ACTCGCT302.1662945E-644.99999617
TGTCGAC302.1662945E-644.99999628
GCGATCT1800.043.759
TACGTAG1250.043.1999971
CGCATCG1100.042.95454421
CGTTTTT96650.042.3228151
TCGTTAG556.184564E-1140.9090921
CTTCGTA501.0822987E-940.50000441
TACGGGA4150.040.120484
CCCAACG451.929584E-840.013
TTAGTCG451.929584E-840.01
TATCGCG451.929584E-840.01
CCGAACA403.4598997E-739.37519
TCGACAC403.4598997E-739.37536
Produced by FastQC (version 0.11.5)