##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545451_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1971196
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.976009488655617	31.0	31.0	33.0	30.0	34.0
2	31.267400603491485	31.0	31.0	34.0	30.0	34.0
3	31.344754149257607	31.0	31.0	34.0	28.0	34.0
4	35.18655171784034	37.0	35.0	37.0	32.0	37.0
5	35.04554392358751	37.0	35.0	37.0	32.0	37.0
6	35.10472626770753	37.0	35.0	37.0	32.0	37.0
7	35.60313839922565	37.0	35.0	37.0	35.0	37.0
8	35.541993794630265	37.0	35.0	37.0	35.0	37.0
9	37.25595476046015	39.0	37.0	39.0	34.0	39.0
10	36.604958106652	39.0	35.0	39.0	32.0	39.0
11	36.30475609731351	38.0	35.0	39.0	32.0	39.0
12	35.521893307413364	37.0	35.0	39.0	30.0	39.0
13	35.04078894234769	37.0	34.0	39.0	29.0	39.0
14	36.006841024433896	38.0	34.0	40.0	29.0	41.0
15	36.3789886951881	38.0	35.0	40.0	31.0	41.0
16	36.4743328415845	38.0	35.0	40.0	31.0	41.0
17	36.41087999366882	38.0	35.0	40.0	31.0	41.0
18	36.34483024519125	38.0	35.0	40.0	31.0	41.0
19	36.2633243979797	37.0	35.0	40.0	31.0	41.0
20	36.13437933112689	37.0	34.0	40.0	30.0	41.0
21	35.9549207689139	37.0	34.0	40.0	30.0	41.0
22	35.83930010004079	37.0	34.0	40.0	30.0	41.0
23	35.794070199006086	37.0	34.0	40.0	30.0	41.0
24	35.691879447807324	36.0	34.0	40.0	30.0	41.0
25	35.57123238886442	36.0	34.0	40.0	29.0	41.0
26	35.44032759806736	36.0	34.0	40.0	29.0	41.0
27	35.34200911527824	36.0	34.0	40.0	29.0	41.0
28	35.3773125554232	36.0	34.0	40.0	29.0	41.0
29	35.432745906546074	36.0	34.0	40.0	29.0	41.0
30	35.31853351975146	36.0	34.0	40.0	29.0	41.0
31	35.14788635934732	36.0	34.0	40.0	28.0	41.0
32	34.92533365530368	36.0	34.0	40.0	27.0	41.0
33	34.66701281861367	36.0	33.0	40.0	25.0	41.0
34	34.48026629518323	36.0	33.0	40.0	24.0	41.0
35	34.27946789664752	36.0	33.0	40.0	24.0	41.0
36	34.10325710888212	36.0	33.0	40.0	23.0	41.0
37	34.034301510352094	36.0	33.0	40.0	23.0	41.0
38	34.030052820724066	35.0	33.0	40.0	23.0	41.0
39	33.97203474438869	36.0	33.0	40.0	23.0	41.0
40	33.79554899664975	35.0	33.0	40.0	23.0	41.0
41	33.78493767235729	35.0	33.0	40.0	23.0	41.0
42	33.74155030752903	35.0	33.0	40.0	23.0	41.0
43	33.626804234586515	35.0	33.0	40.0	23.0	41.0
44	33.58111978717489	35.0	33.0	40.0	23.0	41.0
45	33.50524047329642	35.0	32.0	40.0	23.0	41.0
46	33.47457330473479	35.0	32.0	39.0	23.0	41.0
47	33.40759772239797	35.0	32.0	39.0	23.0	41.0
48	33.38647146199566	35.0	32.0	39.0	23.0	41.0
49	33.41430786182602	35.0	32.0	39.0	22.0	41.0
50	33.30626178218706	35.0	32.0	39.0	22.0	41.0
51	33.12262555321744	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	7.0
12	7.0
13	15.0
14	25.0
15	85.0
16	257.0
17	676.0
18	1467.0
19	2861.0
20	4726.0
21	7461.0
22	11097.0
23	16124.0
24	22559.0
25	31346.0
26	40066.0
27	45992.0
28	49919.0
29	55072.0
30	63898.0
31	77093.0
32	95521.0
33	121912.0
34	193588.0
35	255484.0
36	145334.0
37	173707.0
38	234777.0
39	320031.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.71896858556937	22.923747816046706	27.788104277809005	16.56917932057492
2	33.18330597261764	25.080661689654406	28.65564865188444	13.080383685843518
3	28.424722858609698	25.555246662432353	32.60614368129806	13.413886797659899
4	25.874545199969965	27.18309087477856	31.121461285432805	15.820902639818668
5	22.349172786470753	31.67041735068456	31.429649816659534	14.55076004618516
6	21.17247599934253	39.327646768763735	29.42670338210914	10.073173849784597
7	84.47171159032384	4.917572884685237	8.74540126907725	1.8653142559136686
8	86.1882836612899	3.6267829277250967	8.40575975194755	1.7791736590374574
9	81.98307017668462	4.622878699023334	10.284162508446649	3.1098886158454055
10	48.332027865316284	21.855462369038896	17.34353154125719	12.46897822438763
11	42.88751600551137	23.256540699149145	21.374941913437322	12.481001381902153
12	37.75180144440228	22.828627899001418	25.952365974768615	13.467204681827683
13	22.64026509794054	36.98079744480001	25.73544183328294	14.64349562397651
14	16.993236593418413	39.48059959537255	29.076915740494602	14.449248070714429
15	15.61042128738086	23.64823183488603	46.69297218541434	14.048374692318774
16	17.235526046116163	19.302443795543418	45.01972406599851	18.44230609234191
17	17.602004062508243	19.686829721651222	31.102640224513443	31.60852599132709
18	21.82147285201472	23.270440889693365	35.283858124712104	19.624228133579816
19	28.676296015211072	23.477371098561484	28.600961040911205	19.245371845316246
20	31.16087897905637	23.107341938599713	26.95733960499108	18.774439477352836
21	23.68369253996051	27.170205296682827	29.619327555453644	19.526774607903018
22	22.701192575471946	25.122311530664632	27.329195067360118	24.8473008265033
23	20.01033890085004	29.93776367241005	26.94973001162746	23.102167415112447
24	19.286818763836777	23.886158454055302	39.57683558611117	17.250187195996745
25	18.352157776294188	24.71661874313868	36.35807905454354	20.573144426023593
26	17.05051146613528	32.72383872532209	30.1905543639496	20.03509544459303
27	18.334960095292402	33.004987834796744	30.46439826379518	18.19565380611568
28	16.161710961264124	28.258985915149992	37.81754833106398	17.7617547925219
29	17.401770295800112	24.7964687428343	37.91911103715714	19.88264992420845
30	18.642945704029433	29.63677888956755	33.431175793782046	18.28909961262097
31	26.53830466376758	27.24746803463481	27.843958693098	18.37026860849961
32	29.521265262307754	25.987420834863705	28.442123462101183	16.049190440727358
33	27.617040618994764	26.453685985564096	27.198715906485198	18.73055748895594
34	21.06299931615121	26.64899888189708	29.82022082025329	22.46778098169842
35	21.065282194160297	26.165130205215515	31.53430709072056	21.23528050990363
36	28.848374286473792	25.493608956187003	27.391086426717585	18.266930330621612
37	20.751361102599642	32.589047461541114	28.688775748327412	17.970815687531832
38	21.829995596581973	32.39109657284207	25.290280621511002	20.48862720906495
39	20.888942550613944	31.365374118048127	27.252388905009955	20.493294426327978
40	23.58456490374372	25.902649964792946	27.522377277551296	22.99040785391204
41	18.345765717868744	25.67035444471275	28.788004845789057	27.19587499162945
42	22.445814622188763	25.997668420593385	26.18166838812579	25.374848569092062
43	21.351352174010092	27.0040117776213	27.15757337169921	24.487062676669392
44	20.281494077707137	30.331940608645713	28.60694725435725	20.77961805928989
45	17.69676886519656	35.50057934370808	25.482143835519146	21.32050795557621
46	20.948956876941715	32.230077577267814	27.5146154923204	19.306350053470076
47	21.8239586525135	28.239454625516693	28.04789579524309	21.888690926726717
48	22.558690257082503	25.823104348831876	30.87039543505567	20.74780995902995
49	20.936781527559916	25.9714406888001	32.26127691005867	20.830500873581318
50	19.286463649479806	33.144091201483775	27.95972597346991	19.60971917556651
51	18.932211713091952	34.04973427299974	25.353034401449676	21.66501961245863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	919.0
1	1922.5
2	2926.0
3	11089.5
4	19253.0
5	14813.5
6	10374.0
7	10968.5
8	11563.0
9	12133.5
10	12704.0
11	12771.5
12	12839.0
13	12575.5
14	12312.0
15	11893.5
16	11475.0
17	11244.5
18	11014.0
19	10491.5
20	9969.0
21	10292.5
22	10616.0
23	10916.5
24	11217.0
25	11605.0
26	15371.0
27	18749.0
28	23312.5
29	27876.0
30	30100.5
31	32325.0
32	38721.5
33	45118.0
34	50578.5
35	56039.0
36	58702.0
37	61365.0
38	74833.0
39	88301.0
40	110941.5
41	133582.0
42	159641.0
43	185700.0
44	184028.0
45	182356.0
46	177499.0
47	172642.0
48	160482.0
49	148322.0
50	142783.0
51	137244.0
52	127520.5
53	117797.0
54	105724.0
55	93651.0
56	87832.0
57	82013.0
58	77126.0
59	72239.0
60	65686.5
61	59134.0
62	51178.0
63	43222.0
64	35525.0
65	27828.0
66	21970.0
67	16112.0
68	12465.5
69	8819.0
70	6910.5
71	5002.0
72	3908.5
73	2815.0
74	2258.0
75	1334.0
76	967.0
77	776.5
78	586.0
79	430.5
80	275.0
81	207.0
82	139.0
83	97.0
84	55.0
85	41.0
86	27.0
87	21.0
88	15.0
89	9.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1971196.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.475300804711722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32950634674668	14.38732888512821
2	6.121565028419357	2.1395238053446386
3	2.0727930742891187	1.0866804743737657
4	1.0890885335767935	0.7612859890886738
5	0.649849842721437	0.5678160739725857
6	0.4463712164272945	0.46802827665792296
7	0.3524562149578014	0.4311494863814399
8	0.28961754688854957	0.4048923000160084
9	0.234385019122717	0.36863538419588304
>10	3.360988452250228	14.310093734740814
>50	1.3794635347070563	17.669371631306177
>100	1.6695377966139415	46.16325160927505
>500	0.003793740841796492	0.42052546356226783
>1k	0.0	0.0
>5k	5.836524371994603E-4	0.8214168859565179
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9956	0.5050740768548637	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6151	0.31204405853096295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010044663239982224	0.0
2	0.0	0.0	0.0	0.03287344333085092	0.0
3	0.0	0.0	0.0	0.0498174712205179	0.0
4	0.0	0.0	0.0	0.08411137197924509	0.0
5	0.0	0.0	0.0	0.14209647341005155	0.0
6	0.0	0.0	0.0	0.22351912240081656	0.0
7	0.0	0.0	0.0	0.27054640938800606	0.0
8	0.0	0.0	0.0	0.40995415980957756	0.0
9	0.0	0.0	0.0	0.48072337809127047	0.0
10	0.0	0.0	0.0	0.5923307474244063	0.0
11	0.0	0.0	0.0	0.6787757280351624	0.0
12	0.0	0.0	0.0	0.7629378306368316	0.0
13	0.0	0.0	0.0	0.7984999969561627	0.0
14	0.0	0.0	0.0	0.8119943425209872	0.0
15	0.0	0.0	0.0	0.8315763627767102	0.0
16	0.0	0.0	0.0	0.8772846535808717	0.0
17	0.0	0.0	0.0	0.9272035860462379	0.0
18	0.0	0.0	0.0	1.00700285511943	0.0
19	0.0	0.0	0.0	1.0391660697363427	0.0
20	0.0	0.0	0.0	1.0768589221974882	0.0
21	0.0	0.0	0.0	1.1204365268598353	0.0
22	0.0	0.0	0.0	1.1700003449682326	0.0
23	0.0	0.0	0.0	1.2259054908796487	0.0
24	0.0	0.0	0.0	1.268214829981392	0.0
25	0.0	0.0	0.0	1.3052481843510235	0.0
26	0.0	0.0	0.0	1.3409625425376268	0.0
27	0.0	0.0	0.0	1.3800251218042245	0.0
28	0.0	0.0	0.0	1.416094594347797	0.0
29	0.0	0.0	0.0	1.4623101913762	0.0
30	0.0	0.0	0.0	1.5273468493239637	0.0
31	0.0	0.0	0.0	1.5820851909196245	0.0
32	0.0	0.0	0.0	1.6328158133437771	0.0
33	0.0	0.0	0.0	1.6860829668891373	0.0
34	0.0	0.0	0.0	1.7415822678211603	0.0
35	0.0	0.0	0.0	1.8133660985513362	0.0
36	0.0	0.0	0.0	1.8681551707694213	0.0
37	0.0	0.0	0.0	1.932532330625671	0.0
38	0.0	0.0	0.0	1.9948295349625305	0.0
39	0.0	0.0	0.0	2.073157615985422	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	25	3.891824E-5	45.000004	1
CTAACCG	25	3.891824E-5	45.000004	1
ACCTACG	25	3.891824E-5	45.000004	29
CTACGCG	25	3.891824E-5	45.000004	1
CGACGAT	20	7.034516E-4	45.0	14
TAGCACG	30	2.1662945E-6	44.999996	1
ACTCGCT	30	2.1662945E-6	44.999996	17
TGTCGAC	30	2.1662945E-6	44.999996	28
GCGATCT	180	0.0	43.75	9
TACGTAG	125	0.0	43.199997	1
CGCATCG	110	0.0	42.954544	21
CGTTTTT	9665	0.0	42.322815	1
TCGTTAG	55	6.184564E-11	40.909092	1
CTTCGTA	50	1.0822987E-9	40.500004	41
TACGGGA	415	0.0	40.12048	4
CCCAACG	45	1.929584E-8	40.0	13
TTAGTCG	45	1.929584E-8	40.0	1
TATCGCG	45	1.929584E-8	40.0	1
CCGAACA	40	3.4598997E-7	39.375	19
TCGACAC	40	3.4598997E-7	39.375	36
>>END_MODULE