FastQCFastQC Report
Thu 26 May 2016
SRR1545450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545450_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2062602
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181190.8784535261771297No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26690.12939966120463375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152900.042.8809661
CGGTCTA1300.041.53846431
TATAGCG1250.041.41
CGTTCGA1100.040.90909214
TCGTTAG800.039.3750041
TACGGGA4750.038.842114
TACGGGT6000.038.6254
TAGGGCG8200.037.8658525
CTAAGCG900.037.51
CCCGCTA301.14023656E-437.49999629
AGGGCGA21700.037.4308746
TAGGGTA16800.037.3660745
AGGGTGC13450.037.1375476
GGGCGAT45500.036.8901067
GCGCGAC5000.036.459
GTTTTTT190300.036.3570672
CGTAAGG1550.036.290322
TAAGGGT16900.036.2130174
CGAGGGA9950.036.1809044
TTACGGG5850.036.1538433
Produced by FastQC (version 0.11.5)