##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545450_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2062602
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.909141463064614	31.0	31.0	33.0	28.0	34.0
2	31.20966187369158	31.0	31.0	34.0	30.0	34.0
3	31.29771473119875	31.0	31.0	34.0	28.0	34.0
4	35.16086622625208	35.0	35.0	37.0	32.0	37.0
5	35.057367344742225	37.0	35.0	37.0	32.0	37.0
6	35.115742154812224	37.0	35.0	37.0	32.0	37.0
7	35.596957629246944	37.0	35.0	37.0	35.0	37.0
8	35.52015706374763	37.0	35.0	37.0	35.0	37.0
9	37.24123606977982	39.0	37.0	39.0	34.0	39.0
10	36.36464330006468	38.0	35.0	39.0	32.0	39.0
11	36.277225562663084	38.0	35.0	39.0	32.0	39.0
12	35.94001751186123	37.0	35.0	39.0	31.0	39.0
13	35.69080898787066	37.0	35.0	39.0	30.0	39.0
14	36.74990133821261	38.0	35.0	40.0	31.0	41.0
15	36.95649136382104	39.0	35.0	41.0	31.0	41.0
16	36.912983212466585	38.0	35.0	41.0	31.0	41.0
17	36.83524645084219	38.0	35.0	40.0	31.0	41.0
18	36.73384298085622	38.0	35.0	40.0	31.0	41.0
19	36.64047111367098	38.0	35.0	40.0	31.0	41.0
20	36.50305390957635	38.0	35.0	40.0	31.0	41.0
21	36.37374006230964	38.0	35.0	40.0	30.0	41.0
22	36.28426763864284	38.0	35.0	40.0	30.0	41.0
23	36.18027278166122	38.0	34.0	40.0	30.0	41.0
24	36.05240274177956	38.0	34.0	40.0	30.0	41.0
25	35.92819167246032	38.0	34.0	40.0	30.0	41.0
26	35.81903440411674	38.0	34.0	40.0	30.0	41.0
27	35.73155315470459	38.0	34.0	40.0	29.0	41.0
28	35.747177109301745	38.0	34.0	40.0	29.0	41.0
29	35.73654296854168	38.0	34.0	40.0	29.0	41.0
30	35.584905861625266	37.0	34.0	40.0	29.0	41.0
31	35.42560513370975	38.0	34.0	40.0	28.0	41.0
32	35.181130436216	38.0	34.0	40.0	26.0	41.0
33	34.895267724941604	38.0	34.0	40.0	25.0	41.0
34	34.65863118526987	37.0	33.0	40.0	24.0	41.0
35	34.42435137753188	37.0	33.0	40.0	23.0	41.0
36	34.2619181984697	37.0	33.0	40.0	23.0	41.0
37	34.22596555224905	37.0	33.0	40.0	23.0	41.0
38	34.15078187648417	37.0	33.0	40.0	23.0	41.0
39	34.07394931256733	37.0	33.0	40.0	22.0	41.0
40	33.931599503927565	37.0	33.0	40.0	21.0	41.0
41	33.841426024022084	37.0	33.0	40.0	21.0	41.0
42	33.78588743732431	37.0	33.0	40.0	21.0	41.0
43	33.70218781907513	36.0	33.0	40.0	21.0	41.0
44	33.614929104112186	36.0	33.0	40.0	20.0	41.0
45	33.55008237168392	36.0	32.0	40.0	20.0	41.0
46	33.498440804382035	36.0	32.0	40.0	20.0	41.0
47	33.39842441731366	36.0	32.0	40.0	20.0	41.0
48	33.33060861959797	36.0	32.0	40.0	20.0	41.0
49	33.32612108395124	36.0	32.0	40.0	20.0	41.0
50	33.26451928195551	35.0	32.0	40.0	20.0	41.0
51	33.07868410871317	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	11.0
14	22.0
15	67.0
16	256.0
17	750.0
18	1582.0
19	3028.0
20	5195.0
21	8050.0
22	12130.0
23	16843.0
24	23913.0
25	33665.0
26	43964.0
27	49757.0
28	52194.0
29	55965.0
30	64530.0
31	76364.0
32	93826.0
33	114880.0
34	166030.0
35	207916.0
36	172361.0
37	209758.0
38	286108.0
39	363355.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0759894540973	22.69022331986491	26.904075531779764	18.32971169425803
2	33.96816254420387	24.14998143122134	28.940629360390417	12.941226664184367
3	28.388996035105173	24.602565109507314	34.24858503967319	12.759853815714326
4	24.589280917986116	27.422546860712828	33.55824342262831	14.429928798672744
5	22.05607286330567	30.763569510744198	32.97616311823609	14.204194507714044
6	20.14504009983506	39.53026323061841	30.17310174236232	10.151594927184208
7	84.63101461164102	3.6291053727282336	10.318520005313676	1.4213600103170656
8	85.57952527923469	3.033692394364012	9.955241001414718	1.4315413249865947
9	80.94135465785449	4.078440726810117	12.129436507867247	2.8507681074681397
10	36.30773169036004	22.87222644019544	27.144451522882264	13.675590346562256
11	33.87216729160546	24.541622668842557	25.483442758224804	16.10276728132718
12	29.517861419701912	23.817488783585006	29.55247788957831	17.112171907134773
13	22.528388899070205	29.20791311169096	29.78296346071612	18.48073452852271
14	19.744720503519343	31.081275010884312	31.52498640067255	17.6490180849238
15	20.872034449690247	23.21892444591831	38.43446287747224	17.474578226919203
16	20.789420353514636	21.774923131074246	38.065753839082866	19.36990267632825
17	21.082690698447884	21.866457998198392	32.12059330884	24.930257994513727
18	23.386819173063927	22.586616322489746	34.67043084414734	19.356133660298983
19	26.717660508425766	23.839693746054742	30.06639186813549	19.376253877384002
20	28.351131241024685	23.772060727178584	29.63358902977889	18.24321900201784
21	24.400490254542564	26.052723695603902	31.311809064472936	18.2349769853806
22	23.61284435872747	23.01151652136476	30.733025566735613	22.642613553172158
23	21.588556590171056	27.409941423502936	30.146436394418313	20.8550655919077
24	20.59427848901533	24.395981386617485	36.81990030068816	18.189839823679023
25	21.003858233435242	25.584286255903947	34.02886257261459	19.382992938046215
26	20.802122755626147	28.815641602209247	29.812731685511796	20.56950395665281
27	19.393368182518973	28.469089043838803	31.907270525287963	20.230272248354265
28	18.50114564031258	27.283790086502393	35.33430104305145	18.88076323013359
29	19.745156845576606	24.998084943193113	34.7793224286605	20.477435782569785
30	20.284427145905997	27.14963914511864	33.940818441948565	18.625115267026796
31	24.627097229615796	26.16142135031383	30.799107147185932	18.412374272884442
32	25.18411210694065	25.72168552149179	30.414932206989036	18.679270164578526
33	24.310749238098285	26.85084180079337	28.72425218243752	20.114156778670825
34	20.609308048765588	27.52896584023481	30.541956228104112	21.31976988289549
35	21.285589755076355	27.084963555741727	30.95342678810551	20.676019901076405
36	24.98421896226223	28.507050802820906	27.39001513622114	19.118715098695723
37	21.25160355706045	30.253388680899175	29.04632110315029	19.448686658890082
38	22.017674762266303	30.21033626458231	27.574587826444464	20.197401146706927
39	21.50860902878985	30.26720617937925	27.811570045990454	20.41261474584045
40	23.777054419611733	27.043995884809576	28.416534067163706	20.76241562841498
41	20.571879596742367	26.305850571268717	28.709755929646146	24.41251390234277
42	21.932733508451946	27.250385677896173	27.57032137077342	23.24655944287846
43	21.683776123556555	27.291256383926708	28.383856895319603	22.641110597197134
44	21.13000957043579	27.90295946576218	29.834694235727493	21.132336728074538
45	20.07498295841854	31.623987565221018	27.059849646223554	21.241179830136886
46	21.69570280645515	29.864995767482043	28.037692196555614	20.401609229507194
47	21.380760806011047	28.771619536876237	28.694241545387815	21.153378111724898
48	21.85365863118527	27.931127769681208	29.95643366970458	20.258779929428943
49	21.507057590364013	27.129034103525544	30.63562432306378	20.728283983046655
50	20.524512242303654	30.492504128280686	29.000990011645484	19.981993617770176
51	20.28399080384873	30.44431257217825	28.061594044803602	21.21010257916942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1406.0
1	2264.0
2	3122.0
3	18596.0
4	34070.0
5	25077.5
6	16085.0
7	16177.5
8	16270.0
9	16551.5
10	16833.0
11	16578.5
12	16324.0
13	15919.0
14	15514.0
15	14677.0
16	13840.0
17	13078.5
18	12317.0
19	11934.0
20	11551.0
21	11293.5
22	11036.0
23	11492.0
24	11948.0
25	13113.0
26	16903.0
27	19528.0
28	23534.0
29	27540.0
30	30712.5
31	33885.0
32	39759.5
33	45634.0
34	55233.0
35	64832.0
36	70440.5
37	76049.0
38	81905.0
39	87761.0
40	104617.0
41	121473.0
42	134043.5
43	146614.0
44	150166.0
45	153718.0
46	155554.0
47	157390.0
48	151657.0
49	145924.0
50	144036.0
51	142148.0
52	138974.0
53	135800.0
54	128332.5
55	120865.0
56	110184.0
57	99503.0
58	90330.5
59	81158.0
60	74414.5
61	67671.0
62	58793.0
63	49915.0
64	41888.0
65	33861.0
66	27703.0
67	21545.0
68	17294.0
69	13043.0
70	10787.5
71	8532.0
72	7267.5
73	6003.0
74	4854.0
75	2840.5
76	1976.0
77	1460.0
78	944.0
79	747.5
80	551.0
81	418.0
82	285.0
83	184.5
84	84.0
85	60.5
86	37.0
87	29.5
88	22.0
89	12.5
90	3.0
91	4.5
92	6.0
93	3.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2062602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.69153085785006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.67453537566283	15.86513514736844
2	5.856551679203332	2.423620395816587
3	2.512276583281569	1.5594854533927414
4	1.5490080000411754	1.2820538732763438
5	1.1024942039560848	1.1406146420879086
6	0.9060389724338149	1.1248400013917426
7	0.7316909844949043	1.0597864608860903
8	0.6024013478525017	0.9971684862320406
9	0.5432410700539754	1.011644042784599
>10	7.185207199946351	35.617048077423085
>50	2.0619973402375527	29.34663003422794
>100	0.2726686296799669	7.275453173236627
>500	9.443065780166463E-4	0.1317427100983028
>1k	7.082299335124848E-4	0.2797029136161965
>5k	0.0	0.0
>10k+	2.3607664450416157E-4	0.8850745881613454
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18119	0.8784535261771297	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2669	0.12939966120463375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0063996835065611305	0.0
2	0.0	0.0	0.0	0.022059515117312987	0.0
3	0.0	0.0	0.0	0.031852970180383806	0.0
4	0.0	0.0	0.0	0.04528260905400072	0.0
5	0.0	0.0	0.0	0.07602048286581706	0.0
6	0.0	0.0	0.0	0.1316298539417687	0.0
7	0.0	0.0	0.0	0.16469488539233454	0.0
8	0.0	0.0	0.0	0.24449699942111955	0.0
9	0.0	0.0	0.0	0.28769486309040715	0.0
10	0.0	0.0	0.0	0.3492675756156544	0.0
11	0.0	0.0	0.0	0.40410122747868954	0.0
12	0.0	0.0	0.0	0.4461839947794097	0.0
13	0.0	0.0	0.0	0.4676617204870353	0.0
14	0.0	0.0	0.0	0.47895813152513184	0.0
15	0.0	0.0	0.0	0.4922423230463269	0.0
16	0.0	0.0	0.0	0.5177925746217642	0.0
17	0.0	0.0	0.0	0.5455730189343363	0.0
18	0.0	0.0	0.0	0.5875103388826346	0.0
19	0.0	0.0	0.0	0.6070002841071618	0.0
20	0.0	0.0	0.0	0.6284780098147873	0.0
21	0.0	0.0	0.0	0.6544646034474901	0.0
22	0.0	0.0	0.0	0.684087380890739	0.0
23	0.0	0.0	0.0	0.7225824468317203	0.0
24	0.0	0.0	0.0	0.7540475574056459	0.0
25	0.0	0.0	0.0	0.7798887036859268	0.0
26	0.0	0.0	0.0	0.8062631569250879	0.0
27	0.0	0.0	0.0	0.8386009516135444	0.0
28	4.848245080728129E-5	0.0	0.0	0.8680297992535642	0.0
29	4.848245080728129E-5	0.0	0.0	0.9033735058920722	0.0
30	4.848245080728129E-5	0.0	0.0	0.9502560358227132	0.0
31	4.848245080728129E-5	0.0	0.0	0.9864724265757524	0.0
32	4.848245080728129E-5	0.0	0.0	1.0270522379014468	0.0
33	4.848245080728129E-5	0.0	0.0	1.0662745406045373	0.0
34	4.848245080728129E-5	0.0	0.0	1.1087451675117157	0.0
35	4.848245080728129E-5	0.0	0.0	1.1588760216464447	0.0
36	4.848245080728129E-5	0.0	0.0	1.2020738853157322	0.0
37	4.848245080728129E-5	0.0	0.0	1.249247309951217	0.0
38	4.848245080728129E-5	0.0	0.0	1.3031113127981064	0.0
39	4.848245080728129E-5	0.0	0.0	1.38044082183572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15290	0.0	42.880966	1
CGGTCTA	130	0.0	41.538464	31
TATAGCG	125	0.0	41.4	1
CGTTCGA	110	0.0	40.909092	14
TCGTTAG	80	0.0	39.375004	1
TACGGGA	475	0.0	38.84211	4
TACGGGT	600	0.0	38.625	4
TAGGGCG	820	0.0	37.865852	5
CTAAGCG	90	0.0	37.5	1
CCCGCTA	30	1.14023656E-4	37.499996	29
AGGGCGA	2170	0.0	37.430874	6
TAGGGTA	1680	0.0	37.366074	5
AGGGTGC	1345	0.0	37.137547	6
GGGCGAT	4550	0.0	36.890106	7
GCGCGAC	500	0.0	36.45	9
GTTTTTT	19030	0.0	36.357067	2
CGTAAGG	155	0.0	36.29032	2
TAAGGGT	1690	0.0	36.213017	4
CGAGGGA	995	0.0	36.180904	4
TTACGGG	585	0.0	36.153843	3
>>END_MODULE