FastQCFastQC Report
Thu 26 May 2016
SRR1545449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545449_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences882984
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.5309269477136619No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31400.35561233272630083No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10780.12208601741367908No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8850.10022831670789052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC302.1649284E-645.00000421
TAGAGTC253.890068E-545.00000416
AACCGTG253.890068E-545.00000420
TCGTTAG302.1649284E-645.0000041
GTACCGT253.890068E-545.00000415
GTACAAC302.1649284E-645.00000441
ATAATCG253.890068E-545.00000445
CACGCTC302.1649284E-645.00000413
TACGGGT850.045.0000044
TGCATCG253.890068E-545.00000424
CTACGTG302.1649284E-645.0000041
CGCTATA302.1649284E-645.00000439
CTACGAA600.045.00000411
TACCTAG302.1649284E-645.0000041
CGAATGT253.890068E-545.00000417
ACCGTTC253.890068E-545.00000417
GCTACGA600.045.00000410
ATACGTA253.890068E-545.00000444
CCGTTTA253.890068E-545.00000423
TGCACGG600.045.0000042
Produced by FastQC (version 0.11.5)