FastQCFastQC Report
Thu 26 May 2016
SRR1545448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545448_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1071964
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145041.3530305122186939No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10940.1020556660484867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG253.8906284E-545.0000041
ACACGAC406.8139343E-945.026
CGTTTTT106300.042.756351
TACGGGA2200.041.931824
GTATACG403.4579352E-739.3751
CGTTAGG1450.038.7931022
TAGGGCG4900.038.1122445
CGTAAGG1600.037.968752
GGGCGAT22700.037.16967
AGGGCGA11850.036.8354456
ACGGGTA1100.036.818185
GTTTTTT128900.036.8134232
TTACACG1300.036.34615734
TAGCATA5900.036.22881330
CGATTCG504.8783477E-836.00000410
ACGGGAT2000.036.0000045
CGTGTAC250.002107045136.00000418
GTTGATC6150.035.85365716
TATGGGC3650.035.753424
AGGGCGT1200.035.6256
Produced by FastQC (version 0.11.5)