##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545448_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1071964
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.938770331839503	31.0	31.0	33.0	28.0	34.0
2	31.23465340253964	31.0	31.0	34.0	30.0	34.0
3	31.25423428398715	31.0	31.0	34.0	28.0	34.0
4	35.136722875021924	35.0	35.0	37.0	32.0	37.0
5	35.039431361500945	35.0	35.0	37.0	32.0	37.0
6	35.09308428268113	37.0	35.0	37.0	32.0	37.0
7	35.576724591497474	37.0	35.0	37.0	35.0	37.0
8	35.48523271303887	37.0	35.0	37.0	35.0	37.0
9	37.15616569213145	39.0	37.0	39.0	34.0	39.0
10	36.56541637592307	39.0	35.0	39.0	32.0	39.0
11	36.32327951311798	38.0	35.0	39.0	32.0	39.0
12	35.936922322018276	37.0	35.0	39.0	31.0	39.0
13	35.65498748092287	37.0	35.0	39.0	30.0	39.0
14	36.70198812646694	38.0	35.0	41.0	31.0	41.0
15	36.910278703389295	39.0	35.0	41.0	31.0	41.0
16	36.90010578713464	38.0	35.0	41.0	31.0	41.0
17	36.786474172640126	38.0	35.0	40.0	31.0	41.0
18	36.648583347948254	38.0	35.0	40.0	31.0	41.0
19	36.50149818464053	38.0	35.0	40.0	31.0	41.0
20	36.317449093439706	38.0	35.0	40.0	30.0	41.0
21	36.171707258825855	38.0	34.0	40.0	30.0	41.0
22	36.095751349858766	38.0	34.0	40.0	30.0	41.0
23	35.9940595019982	38.0	34.0	40.0	30.0	41.0
24	35.84564500300383	37.0	34.0	40.0	30.0	41.0
25	35.68745592202723	37.0	34.0	40.0	29.0	41.0
26	35.555571828904704	37.0	34.0	40.0	29.0	41.0
27	35.47353176039494	37.0	34.0	40.0	29.0	41.0
28	35.44252605497946	37.0	34.0	40.0	29.0	41.0
29	35.396766122742925	37.0	34.0	40.0	28.0	41.0
30	35.227439540880106	37.0	34.0	40.0	27.0	41.0
31	35.03981476989899	37.0	34.0	40.0	26.0	41.0
32	34.730921933945545	37.0	33.0	40.0	25.0	41.0
33	34.35864077524992	37.0	33.0	40.0	23.0	41.0
34	34.02236548988586	37.0	33.0	40.0	21.0	41.0
35	33.762645014198235	37.0	33.0	40.0	20.0	41.0
36	33.58624263501386	36.0	33.0	40.0	19.0	41.0
37	33.513743931699196	36.0	32.0	40.0	18.0	41.0
38	33.43049953170069	36.0	32.0	40.0	18.0	41.0
39	33.31854148087063	36.0	32.0	40.0	18.0	41.0
40	33.164101592963945	36.0	32.0	40.0	18.0	41.0
41	33.04059464683515	36.0	32.0	40.0	18.0	41.0
42	32.976849035975086	36.0	31.0	40.0	18.0	41.0
43	32.89302905694594	35.0	31.0	40.0	17.0	41.0
44	32.78634170550504	35.0	31.0	40.0	17.0	41.0
45	32.71827412114586	35.0	31.0	40.0	18.0	41.0
46	32.66274986846573	35.0	31.0	39.0	18.0	41.0
47	32.55490389602636	35.0	31.0	39.0	18.0	41.0
48	32.4653411868309	35.0	31.0	39.0	17.0	41.0
49	32.44101947453459	35.0	31.0	39.0	17.0	41.0
50	32.380805698698836	35.0	31.0	39.0	17.0	41.0
51	32.207179532148466	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	2.0
13	4.0
14	12.0
15	61.0
16	174.0
17	453.0
18	998.0
19	1926.0
20	3330.0
21	5088.0
22	7486.0
23	10477.0
24	14832.0
25	20870.0
26	27054.0
27	30191.0
28	30944.0
29	32045.0
30	35918.0
31	41729.0
32	49309.0
33	61534.0
34	87667.0
35	106832.0
36	89432.0
37	107771.0
38	138287.0
39	167486.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.02629565918259	22.533032825729222	23.61273326343049	20.827938251657706
2	38.013776582049395	23.445376896985344	26.084644633588443	12.456201887376816
3	28.489482855767545	24.112190334750046	34.7351217018482	12.663205107634212
4	25.462888679097436	26.583448697904032	33.440581959841936	14.5130806631566
5	22.17201323925057	29.64455895906952	33.71456504136333	14.468862760316577
6	20.842957412748937	38.233653368956425	31.28659171390084	9.636797504393805
7	80.41221533558964	4.4723516834520565	13.28841267057476	1.8270203103835578
8	80.39411771290827	4.0637558723986995	13.349049035228793	2.1930773794642358
9	75.13228056166066	5.888350728196096	15.19407368157886	3.7852950285643923
10	40.16478165311522	24.428152437955006	21.848588198857424	13.558477710072353
11	32.288304457985525	24.723498177177593	27.851308439462517	15.13688892537436
12	29.036049718087547	21.97424540376356	32.852129362553214	16.137575515595675
13	22.772126675895834	26.794276673470378	33.035810904097524	17.397785746536265
14	18.983380038881904	29.311805247191135	34.862178207477115	16.842636506449843
15	18.746898216731157	22.57827688243262	41.412771324410144	17.262053576426073
16	21.15238944591423	20.917027064341713	39.38191954207417	18.548663947669887
17	21.257523573552845	20.733158949367702	34.11075371934132	23.898563757738135
18	23.048441925288536	22.381255340664424	35.341018914814306	19.229283819232734
19	27.377691788157065	23.016444582094174	31.1055222003724	18.50034142937636
20	28.414853483885654	22.565776462642404	30.873798000679127	18.14557205279282
21	24.604557615740827	24.114429215906505	32.821344746651945	18.45966842170073
22	23.510491023952294	23.176338011351127	31.791086267822426	21.52208469687415
23	22.55654107787202	24.677321253325673	31.948367669063515	20.817769999738797
24	21.465273087529056	23.061875212227275	36.40430089070155	19.068550809542113
25	21.71434861618487	23.85033452615946	34.391640017761794	20.043676839893877
26	20.344619782007605	26.79940744278726	32.150333406718886	20.705639368486256
27	20.23668705292342	26.55051848756115	32.97041691698602	20.242377542529415
28	19.787511520909284	25.62576728322966	35.758943397352894	18.82777779850816
29	20.644070136683695	23.94809900332474	35.4553884272233	19.952442432768265
30	21.229630845811986	25.63005847211287	34.07922280972122	19.061087872353923
31	25.023228391998238	24.97033482467695	30.927624435148942	19.078812348175873
32	26.225600859730363	24.734412722815318	30.627521073468884	18.41246534398543
33	25.775958894141965	25.7833285446153	29.12243321604084	19.31827934520189
34	21.821628338265093	26.571228138258373	31.008224156781385	20.59891936669515
35	22.52930135713513	26.22289554499965	30.654667507490924	20.593135590374303
36	25.14319510729838	27.891328440134185	27.823508998436512	19.141967454130924
37	22.587512267203003	29.988600363445038	28.721393628890524	18.702493740461435
38	22.664473806956202	29.14342272688262	27.772201305267714	20.419902160893464
39	22.692179961267357	28.866920904060212	27.864928299830964	20.57597083484147
40	23.711710467888846	26.4460373669265	28.867853771208736	20.974398393975918
41	20.460015448279982	26.675615972178168	29.10358929964066	23.76077927990119
42	21.944860088585063	27.12563108462598	28.051501729535694	22.878007097253267
43	21.923870577743283	26.95528954330556	28.84154691762037	22.27929296133079
44	21.570407215167673	27.68563123388472	29.78029112917971	20.963670421767898
45	20.732226082219178	29.304902030292062	28.435376561153173	21.527495326335586
46	21.407528611035445	29.338205387494355	28.324272083764008	20.92999391770619
47	21.701568336250098	28.064935016474436	29.245664966360813	20.987831680914656
48	22.29235310141012	26.752390938501662	30.36734442574564	20.58791153434257
49	21.608561481542292	26.85836464657395	30.86764107749887	20.665432794384884
50	20.439678944442164	29.465168606408426	29.342030142803306	20.753122306346107
51	20.616084122227985	29.64259993805762	28.76467866458202	20.976637275132372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1069.0
1	1736.0
2	2403.0
3	13936.0
4	25469.0
5	18225.5
6	10982.0
7	10931.0
8	10880.0
9	11228.0
10	11576.0
11	11345.5
12	11115.0
13	10745.5
14	10376.0
15	9909.5
16	9443.0
17	9133.5
18	8824.0
19	8371.0
20	7918.0
21	7637.0
22	7356.0
23	7728.0
24	8100.0
25	8648.5
26	9983.5
27	10770.0
28	12583.5
29	14397.0
30	16157.5
31	17918.0
32	20017.0
33	22116.0
34	26067.0
35	30018.0
36	32395.5
37	34773.0
38	36768.0
39	38763.0
40	45453.0
41	52143.0
42	58434.5
43	64726.0
44	66521.5
45	68317.0
46	69016.5
47	69716.0
48	67112.0
49	64508.0
50	64775.5
51	65043.0
52	61871.5
53	58700.0
54	56519.5
55	54339.0
56	55505.5
57	56672.0
58	54296.5
59	51921.0
60	48897.5
61	45874.0
62	41324.0
63	36774.0
64	31578.5
65	26383.0
66	22239.0
67	18095.0
68	15196.0
69	12297.0
70	10627.5
71	8958.0
72	7574.5
73	6191.0
74	4964.5
75	2835.5
76	1933.0
77	1459.0
78	985.0
79	837.0
80	689.0
81	478.0
82	267.0
83	194.0
84	121.0
85	92.5
86	64.0
87	45.5
88	27.0
89	19.0
90	11.0
91	8.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1071964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.89304261014759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93957010600792	21.074461070387347
2	6.220381488450699	3.594514947943587
3	3.1929872483178094	2.767653498579131
4	2.1660132617340193	2.503308538617032
5	1.6506011865427523	2.3845445207569957
6	1.3967175649877306	2.4213252071719253
7	1.2078973471111343	2.4429880663136365
8	1.0089773263534758	2.3321939906403015
9	0.8761924370706853	2.2784278877075135
>10	9.152686857163442	52.5739958884141
>50	0.16658503195051386	2.946693029204305
>100	0.01944558573629125	0.9606564143368579
>500	0.0012963723823575801	0.2586346638387355
>1k	3.2409309558939503E-4	0.10244254968847816
>5k	0.0	0.0
>10k+	3.2409309558939503E-4	1.3581597264000798
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14504	1.3530305122186939	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1094	0.1020556660484867	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009048811340679351	0.0
2	0.0	0.0	0.0	0.028265874600266427	0.0
3	0.0	0.0	0.0	0.04729636443014877	0.0
4	0.0	0.0	0.0	0.0684724487016355	0.0
5	0.0	0.0	0.0	0.1133433585456228	0.0
6	0.0	0.0	0.0	0.20952196155841055	0.0
7	0.0	0.0	0.0	0.2668932911926147	0.0
8	0.0	0.0	0.0	0.38975189465317867	0.0
9	0.0	0.0	0.0	0.4655939938281509	0.0
10	0.0	0.0	0.0	0.5650376318607715	0.0
11	0.0	0.0	0.0	0.6366818288673873	0.0
12	0.0	0.0	0.0	0.6954524592243769	0.0
13	0.0	0.0	0.0	0.7288491031415234	0.0
14	0.0	0.0	0.0	0.7482527398308152	0.0
15	0.0	0.0	0.0	0.7668167960864357	0.0
16	0.0	0.0	0.0	0.7977879854174207	0.0
17	0.0	0.0	0.0	0.8267068670216537	0.0
18	0.0	0.0	0.0	0.8728837908735742	0.0
19	0.0	0.0	0.0	0.8962987563015176	0.0
20	0.0	0.0	0.0	0.9216727427413607	0.0
21	0.0	0.0	0.0	0.9508714844901508	0.0
22	0.0	0.0	0.0	0.9838949815478878	0.0
23	0.0	0.0	0.0	1.0222358213522096	0.0
24	0.0	0.0	0.0	1.0547928848356847	0.0
25	0.0	0.0	0.0	1.085017780447851	0.0
26	0.0	0.0	0.0	1.1123507878995937	0.0
27	0.0	0.0	0.0	1.1500386206999489	0.0
28	0.0	0.0	0.0	1.182315824038867	0.0
29	0.0	0.0	0.0	1.2215895309917124	0.0
30	0.0	0.0	0.0	1.2708449164337607	0.0
31	0.0	0.0	0.0	1.3129172248321772	0.0
32	0.0	0.0	0.0	1.3600270158326213	0.0
33	0.0	0.0	0.0	1.4013530305122186	0.0
34	0.0	0.0	0.0	1.4499554089503006	0.0
35	0.0	0.0	0.0	1.5085394658775855	0.0
36	0.0	0.0	0.0	1.5620860402028427	0.0
37	0.0	0.0	0.0	1.6136735935162	0.0
38	0.0	0.0	0.0	1.6772018463306604	0.0
39	0.0	0.0	0.0	1.7695556940345012	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACG	25	3.8906284E-5	45.000004	1
ACACGAC	40	6.8139343E-9	45.0	26
CGTTTTT	10630	0.0	42.75635	1
TACGGGA	220	0.0	41.93182	4
GTATACG	40	3.4579352E-7	39.375	1
CGTTAGG	145	0.0	38.793102	2
TAGGGCG	490	0.0	38.112244	5
CGTAAGG	160	0.0	37.96875	2
GGGCGAT	2270	0.0	37.1696	7
AGGGCGA	1185	0.0	36.835445	6
ACGGGTA	110	0.0	36.81818	5
GTTTTTT	12890	0.0	36.813423	2
TTACACG	130	0.0	36.346157	34
TAGCATA	590	0.0	36.228813	30
CGATTCG	50	4.8783477E-8	36.000004	10
ACGGGAT	200	0.0	36.000004	5
CGTGTAC	25	0.0021070451	36.000004	18
GTTGATC	615	0.0	35.853657	16
TATGGGC	365	0.0	35.75342	4
AGGGCGT	120	0.0	35.625	6
>>END_MODULE