FastQCFastQC Report
Thu 26 May 2016
SRR1545447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545447_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1479335
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203571.3760912842594815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG207.0339453E-445.01
CGTTTTT163250.042.6845321
TAGGACG501.0822987E-940.51
CGTAAGG2300.039.1304322
CGCGTAA750.039.031
AGTTACG356.2495055E-638.5714261
GTTTTTT191300.037.9665452
GGGCGAT37050.037.7732777
AGGGCGA19450.037.7120826
AGGGTAC7250.037.5517276
GCGATAT2350.037.3404279
TAGGGCG8650.037.196535
TATGGGC6050.036.8181844
ATAGGGC8900.036.404494
GTTGATC10050.036.26865816
GGCGATA8150.036.165648
AATACGG1250.036.0000042
CGTAGCG250.002107303836.01
TATAGCG751.8189894E-1236.01
TTACGCG250.002107303836.01
Produced by FastQC (version 0.11.5)