##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545447_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1479335
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.848513014293584	31.0	31.0	33.0	28.0	34.0
2	31.156498021070277	31.0	31.0	34.0	28.0	34.0
3	31.181152342099658	31.0	31.0	34.0	28.0	34.0
4	35.05855198450655	35.0	35.0	37.0	32.0	37.0
5	34.96065394248091	35.0	35.0	37.0	32.0	37.0
6	35.013817695113005	37.0	35.0	37.0	32.0	37.0
7	35.52339328144065	37.0	35.0	37.0	35.0	37.0
8	35.431815646895394	37.0	35.0	37.0	35.0	37.0
9	37.1262499704259	39.0	37.0	39.0	34.0	39.0
10	36.4710373242031	38.0	35.0	39.0	32.0	39.0
11	36.22546887621803	38.0	35.0	39.0	31.0	39.0
12	35.86212656362487	37.0	35.0	39.0	30.0	39.0
13	35.58537788938949	37.0	35.0	39.0	30.0	39.0
14	36.62543980910341	38.0	35.0	40.0	30.0	41.0
15	36.814638334116346	38.0	35.0	41.0	31.0	41.0
16	36.78428483068406	38.0	35.0	41.0	31.0	41.0
17	36.66647311122903	38.0	35.0	40.0	31.0	41.0
18	36.49844964122393	38.0	35.0	40.0	30.0	41.0
19	36.331459067756796	38.0	35.0	40.0	30.0	41.0
20	36.15128419188351	38.0	34.0	40.0	30.0	41.0
21	36.007124146998486	38.0	34.0	40.0	30.0	41.0
22	35.921194320421	37.0	34.0	40.0	30.0	41.0
23	35.810087640730465	37.0	34.0	40.0	30.0	41.0
24	35.66893570421845	37.0	34.0	40.0	29.0	41.0
25	35.52733086150196	37.0	34.0	40.0	29.0	41.0
26	35.389404698732875	37.0	34.0	40.0	28.0	41.0
27	35.27974731889667	37.0	34.0	40.0	27.0	41.0
28	35.26731605755289	37.0	34.0	40.0	27.0	41.0
29	35.21829808664028	37.0	34.0	40.0	27.0	41.0
30	35.014689032572065	37.0	34.0	40.0	26.0	41.0
31	34.807398594638805	37.0	33.0	40.0	25.0	41.0
32	34.45759817755951	37.0	33.0	40.0	24.0	41.0
33	34.04079535737341	37.0	33.0	40.0	22.0	41.0
34	33.65865743729446	36.0	33.0	40.0	20.0	41.0
35	33.33916928890346	36.0	32.0	40.0	18.0	41.0
36	33.16751580946844	36.0	32.0	40.0	18.0	41.0
37	33.1261377578439	36.0	32.0	40.0	18.0	41.0
38	33.02394589460805	36.0	31.0	40.0	17.0	41.0
39	32.90949108890143	36.0	31.0	40.0	16.0	41.0
40	32.752870715558004	36.0	31.0	40.0	15.0	41.0
41	32.60773928826128	35.0	31.0	40.0	15.0	41.0
42	32.52797642183819	35.0	31.0	40.0	15.0	41.0
43	32.417068480094095	35.0	31.0	40.0	15.0	41.0
44	32.30313823440938	35.0	31.0	40.0	14.0	41.0
45	32.23653939101015	35.0	30.0	39.0	14.0	41.0
46	32.15268887709681	35.0	30.0	39.0	14.0	41.0
47	32.05093707645665	35.0	30.0	39.0	14.0	41.0
48	31.957121274085992	35.0	30.0	39.0	13.0	41.0
49	31.960542405878318	35.0	30.0	39.0	13.0	41.0
50	31.907632821504258	35.0	30.0	39.0	13.0	41.0
51	31.73898947838049	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	11.0
14	25.0
15	80.0
16	296.0
17	768.0
18	1755.0
19	3412.0
20	5403.0
21	8236.0
22	12054.0
23	16347.0
24	22895.0
25	31637.0
26	41687.0
27	46511.0
28	46587.0
29	47461.0
30	51532.0
31	59083.0
32	69376.0
33	83899.0
34	118604.0
35	139006.0
36	122226.0
37	145398.0
38	187417.0
39	217580.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.72964203510361	22.687153349308982	25.25797064221424	22.32523397337317
2	36.96681279088239	23.472438629519345	26.85206528609139	12.708683293506882
3	27.992172158436052	23.654479884542717	35.627224394744935	12.726123562276292
4	24.61984607948842	26.18298086640281	35.00309260579923	14.194080448309546
5	21.58578009713824	29.609452896064788	34.790564679399864	14.014202327397108
6	20.111942190240885	37.52165669033721	32.45634017987812	9.910060939543781
7	80.21191954493067	3.962320907705151	14.169069210151857	1.6566903372123285
8	80.75777291823691	3.489676104465858	13.985405604545287	1.7671453727519457
9	76.11338878617757	4.930864205876289	15.753024162884	3.202722845062139
10	38.272331824772614	24.782824715159176	23.12978466675905	13.815058793309158
11	31.67112249760872	24.91166639064174	28.402424062163067	15.01478704958647
12	28.58405972954064	22.03915948720202	32.95014313864	16.426637644617344
13	23.110722047406437	25.976807146454316	33.22628072748904	17.686190078650206
14	20.254911835385496	27.4426684963176	34.42039835466611	17.882021313630787
15	19.83539901374604	22.41094816251897	39.74461497902774	18.00903784470725
16	22.227960536322065	20.817461900110523	38.19837967735503	18.756197886212387
17	21.923431812267	21.346348190234128	34.085720948939894	22.64449904855898
18	23.75702596098923	21.8371092416525	35.4511993564676	18.95466544089067
19	26.70179506332237	23.276472198656826	31.41404752811229	18.607685209908507
20	28.405398371565603	22.59670730429551	31.317382472529886	17.680511851608998
21	25.206596207079535	24.166331493542707	32.59342880415863	18.033643495219135
22	24.179107504385417	22.195040339071273	32.43998147816418	21.18587067837914
23	22.519307661888618	24.753757600543487	32.41463225030166	20.312302487266237
24	21.924310585499565	22.521943981586322	37.10295504398936	18.450790388924755
25	21.887807697377536	23.538481817843827	34.480492924185526	20.093217560593104
26	20.453717379768612	25.738389208664703	32.74728171779888	21.060611693767807
27	20.193668100869647	25.391949761210274	33.98506761484045	20.42931452307963
28	19.211605214505166	25.6847840414781	35.7507934308321	19.35281731318464
29	20.78373052756813	23.628995460798265	35.65913062288122	19.92814338875238
30	21.26408149607763	24.67338364873406	35.459513903206506	18.6030209519818
31	24.683590937820036	24.54190565355379	32.07664254546806	18.69786086315811
32	25.4248023605201	24.031068013668303	31.40951846606752	19.134611159744075
33	24.57387947963105	25.466442692155596	29.862674782926113	20.09700304528724
34	21.45599205048214	26.38354395725106	31.81044185394113	20.350022138325667
35	21.743012907826827	26.55152484055336	31.55627359590627	20.149188655713544
36	23.92980629810016	28.9255645273045	27.77680511851609	19.367824056079254
37	22.04186340484069	29.22400943667256	28.983766354476842	19.750360804009908
38	22.826337509759455	28.99769153031599	28.141022824444768	20.034948135479794
39	21.961827442736094	28.73967018964602	27.631942731024413	21.66655963659347
40	24.18809803053399	26.18081773229187	28.748119932266864	20.882964304907272
41	20.456691689171148	26.141881318295045	29.490142530258527	23.91128446227528
42	22.351123984763426	26.65616645316984	27.456864063920612	23.535845498146127
43	21.989069412945682	26.61635126594044	28.781716108927323	22.612863212186557
44	21.615725984986497	27.19363768179621	29.13153545343009	22.059100879787202
45	21.120435871523355	29.06765539921654	27.886448978764104	21.925459750496
46	21.790872250031263	28.881220278030327	28.171779887584623	21.156127584353783
47	21.4447031943407	28.538431119388104	29.502715747278334	20.51414993899286
48	21.8387315922357	27.051614407825138	30.38486887689401	20.72478512304515
49	21.830755035201626	26.875183781902003	30.513102170907874	20.780959011988497
50	20.829697127425497	28.343613853522022	30.106500555993065	20.720188463059415
51	20.56248246678406	28.51335228328945	29.17919200181163	21.744973248114864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1122.0
1	1926.0
2	2730.0
3	20316.5
4	37903.0
5	27508.0
6	17113.0
7	17034.0
8	16955.0
9	17221.0
10	17487.0
11	17281.5
12	17076.0
13	16527.5
14	15979.0
15	15101.5
16	14224.0
17	13573.0
18	12922.0
19	12140.5
20	11359.0
21	11299.0
22	11239.0
23	10677.5
24	10116.0
25	10599.0
26	12050.5
27	13019.0
28	14737.5
29	16456.0
30	19178.0
31	21900.0
32	25367.5
33	28835.0
34	34391.5
35	39948.0
36	42477.0
37	45006.0
38	49790.0
39	54574.0
40	61093.5
41	67613.0
42	75059.5
43	82506.0
44	84675.5
45	86845.0
46	89273.0
47	91701.0
48	91123.5
49	90546.0
50	91914.5
51	93283.0
52	91424.0
53	89565.0
54	86631.0
55	83697.0
56	81086.0
57	78475.0
58	76805.5
59	75136.0
60	69591.5
61	64047.0
62	57677.0
63	51307.0
64	44424.5
65	37542.0
66	31133.0
67	24724.0
68	20561.5
69	16399.0
70	13596.5
71	10794.0
72	9417.0
73	8040.0
74	6432.0
75	3671.5
76	2519.0
77	1950.5
78	1382.0
79	1062.5
80	743.0
81	539.5
82	336.0
83	217.5
84	99.0
85	113.0
86	127.0
87	76.0
88	25.0
89	15.5
90	6.0
91	5.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1479335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.36823123952733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53525557602926	20.860651344385396
2	6.115864576963826	3.469925210978876
3	3.178800493525806	2.7053084239399103
4	2.1171765261307582	2.402422130727061
5	1.5504197437053964	2.199133290388169
6	1.2741155541900746	2.168664280028553
7	1.1424188186765873	2.2685880854423557
8	0.9740938852237578	2.2106656468029726
9	0.8894808155901454	2.270969771380615
>10	8.955663059341488	52.06894738550074
>50	0.2343464633551154	4.12266437437402
>100	0.030206855290993387	1.48171480228529
>500	0.0016781582042430078	0.29706381116552766
>1k	2.397368863204297E-4	0.08881990324683588
>5k	0.0	0.0
>10k+	2.397368863204297E-4	1.3844615393536277
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20357	1.3760912842594815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00770616527020587	0.0
2	6.759794096671816E-5	0.0	0.0	0.021766536991283246	0.0
3	6.759794096671816E-5	0.0	0.0	0.03602970253526078	0.0
4	6.759794096671816E-5	0.0	0.0	0.04576380603446819	0.0
5	6.759794096671816E-5	0.0	0.0	0.07456052888629013	0.0
6	6.759794096671816E-5	0.0	0.0	0.13208637664896727	0.0
7	6.759794096671816E-5	0.0	0.0	0.17305072887479847	0.0
8	6.759794096671816E-5	0.0	0.0	0.2602520727218649	0.0
9	6.759794096671816E-5	0.0	0.0	0.3090577860998354	0.0
10	1.351958819334363E-4	0.0	0.0	0.37719651059428727	0.0
11	1.351958819334363E-4	0.0	0.0	0.4329648118918298	0.0
12	1.351958819334363E-4	0.0	0.0	0.47203642177059285	0.0
13	2.0279382290015445E-4	0.0	0.0	0.49664207228247825	0.0
14	2.0279382290015445E-4	0.0	0.0	0.5121220007638567	0.0
15	2.0279382290015445E-4	0.0	0.0	0.5261147745439674	0.0
16	2.703917638668726E-4	0.0	0.0	0.5467321465388164	0.0
17	2.703917638668726E-4	0.0	0.0	0.5671467247107653	0.0
18	2.703917638668726E-4	0.0	0.0	0.6020272622495919	0.0
19	2.703917638668726E-4	0.0	0.0	0.6173043969080702	0.0
20	2.703917638668726E-4	0.0	0.0	0.6377189750800191	0.0
21	2.703917638668726E-4	0.0	0.0	0.660026295599036	0.0
22	2.703917638668726E-4	0.0	0.0	0.6842263584651211	0.0
23	2.703917638668726E-4	6.083814687004634E-4	0.0	0.7147130298411111	0.0
24	2.703917638668726E-4	6.083814687004634E-4	0.0	0.7383047112384957	0.0
25	2.703917638668726E-4	6.083814687004634E-4	0.0	0.7624371761636141	0.0
26	2.703917638668726E-4	6.083814687004634E-4	0.0	0.7844065069777975	0.0
27	2.703917638668726E-4	6.083814687004634E-4	0.0	0.8132032298296193	0.0
28	2.703917638668726E-4	6.083814687004634E-4	0.0	0.8419323547404746	0.0
29	2.703917638668726E-4	6.083814687004634E-4	0.0	0.8723514281754978	0.0
30	2.703917638668726E-4	6.083814687004634E-4	0.0	0.9157493062761308	0.0
31	2.703917638668726E-4	6.083814687004634E-4	0.0	0.9513734211655913	0.0
32	2.703917638668726E-4	6.083814687004634E-4	0.0	0.9883494948743862	0.0
33	2.703917638668726E-4	6.083814687004634E-4	0.0	1.0252579706422142	0.0
34	2.703917638668726E-4	6.083814687004634E-4	0.0	1.0649379619896777	0.0
35	2.703917638668726E-4	6.083814687004634E-4	0.0	1.1112425515518798	0.0
36	2.703917638668726E-4	6.083814687004634E-4	0.0	1.1530180790693116	0.0
37	2.703917638668726E-4	6.083814687004634E-4	0.0	1.2031757512666164	0.0
38	2.703917638668726E-4	6.083814687004634E-4	0.0	1.2611747846160606	0.0
39	2.703917638668726E-4	6.083814687004634E-4	0.0	1.3484437264040938	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	20	7.0339453E-4	45.0	1
CGTTTTT	16325	0.0	42.684532	1
TAGGACG	50	1.0822987E-9	40.5	1
CGTAAGG	230	0.0	39.130432	2
CGCGTAA	75	0.0	39.0	31
AGTTACG	35	6.2495055E-6	38.571426	1
GTTTTTT	19130	0.0	37.966545	2
GGGCGAT	3705	0.0	37.773277	7
AGGGCGA	1945	0.0	37.712082	6
AGGGTAC	725	0.0	37.551727	6
GCGATAT	235	0.0	37.340427	9
TAGGGCG	865	0.0	37.19653	5
TATGGGC	605	0.0	36.818184	4
ATAGGGC	890	0.0	36.40449	4
GTTGATC	1005	0.0	36.268658	16
GGCGATA	815	0.0	36.16564	8
AATACGG	125	0.0	36.000004	2
CGTAGCG	25	0.0021073038	36.0	1
TATAGCG	75	1.8189894E-12	36.0	1
TTACGCG	25	0.0021073038	36.0	1
>>END_MODULE