FastQCFastQC Report
Thu 26 May 2016
SRR1545446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545446_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1800160
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134920.7494889343169495No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33480.18598346813616567No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28440.15798595680383964No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24730.13737667762865524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG207.0343516E-445.00000437
TATTACG453.8562575E-1045.01
TACGTAG453.8562575E-1045.01
ATACCGG302.1661908E-644.9999962
ATTACGG1400.043.3928572
CGTTTTT100800.040.7366031
TCGATTG451.929402E-840.01
CGCATCG850.039.70588321
CACGACG1550.039.19354626
ACTCACG356.2502622E-638.57142642
TATAGCG1000.038.251
TATACTA11100.037.90540344
ATAGGGC9300.037.7419364
CTATGCG601.5643309E-1037.4999961
AACGCGT301.1401788E-437.49999632
TGGGCGA11050.037.466066
GTTGATC10700.037.21962716
ATAACGC1150.037.17391211
TACGGGA4800.037.0312464
CGAGGGA8100.036.9444474