FastQCFastQC Report
Thu 26 May 2016
SRR1545445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545445_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1520242
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85250.5607659833105518No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54300.3571799752934072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG207.034005E-445.0000041
CCGTAAG406.8175723E-945.0000041
TACGAGT207.034005E-445.00000438
TCGTATA207.034005E-445.00000422
CATCGTA207.034005E-445.00000435
ACCCGTA207.034005E-445.00000433
TTGCAAC207.034005E-445.00000427
GTCCAAT207.034005E-445.00000436
TCAACTT207.034005E-445.00000439
TACCCGC253.8914026E-545.040
TAACGCG351.2122837E-745.01
CATTACG253.8914026E-545.035
CGTTAGT253.8914026E-545.022
TCGCTCA253.8914026E-545.040
TGTTACG253.8914026E-545.01
TTACGAG351.2122837E-745.01
TAGTGCG1100.045.01
AATACGC253.8914026E-545.017
GTGCCGT351.2122837E-745.041
CTTATCG302.165967E-644.99999627