##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545445_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1520242
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.901296635667215	31.0	31.0	33.0	28.0	34.0
2	31.186881430719584	31.0	31.0	34.0	28.0	34.0
3	31.294557050785336	31.0	31.0	34.0	28.0	34.0
4	35.11101324657522	35.0	35.0	37.0	32.0	37.0
5	34.97201629740528	35.0	35.0	37.0	32.0	37.0
6	35.02136567730664	37.0	35.0	37.0	32.0	37.0
7	35.55067745793104	37.0	35.0	37.0	35.0	37.0
8	35.48037878179921	37.0	35.0	37.0	35.0	37.0
9	37.18088764815076	39.0	37.0	39.0	34.0	39.0
10	36.58894439174816	39.0	35.0	39.0	32.0	39.0
11	36.234078521709044	38.0	35.0	39.0	31.0	39.0
12	35.14956303009652	37.0	34.0	39.0	30.0	39.0
13	34.53052803435243	37.0	33.0	39.0	26.0	39.0
14	35.451889238686995	37.0	33.0	40.0	27.0	41.0
15	35.95373236629431	37.0	34.0	40.0	30.0	41.0
16	36.11387200195758	37.0	34.0	40.0	31.0	41.0
17	36.02831128201957	37.0	34.0	40.0	31.0	41.0
18	35.979692706819044	37.0	34.0	40.0	30.0	41.0
19	35.86553653957725	36.0	34.0	40.0	30.0	41.0
20	35.700935772067865	36.0	34.0	40.0	30.0	41.0
21	35.49831934652509	35.0	34.0	40.0	29.0	41.0
22	35.36174569575107	35.0	34.0	40.0	29.0	41.0
23	35.372251259996766	35.0	34.0	40.0	29.0	41.0
24	35.2512547344436	35.0	34.0	40.0	29.0	41.0
25	35.13506468049166	35.0	34.0	40.0	29.0	41.0
26	34.974831638647004	35.0	34.0	40.0	29.0	41.0
27	34.812833746206195	35.0	34.0	40.0	27.0	41.0
28	34.90890266154994	36.0	34.0	40.0	28.0	41.0
29	34.9957085779764	36.0	34.0	40.0	29.0	41.0
30	34.890295097754176	36.0	34.0	40.0	29.0	41.0
31	34.68897517632061	35.0	33.0	40.0	27.0	41.0
32	34.43120371625044	35.0	33.0	40.0	26.0	41.0
33	34.16811994406154	35.0	33.0	40.0	24.0	41.0
34	34.00674629433998	35.0	33.0	40.0	23.0	41.0
35	33.82693150169513	35.0	33.0	40.0	23.0	41.0
36	33.59622678494608	35.0	33.0	40.0	23.0	41.0
37	33.56315244546592	35.0	33.0	40.0	23.0	41.0
38	33.58308085160126	35.0	33.0	39.0	23.0	41.0
39	33.53563906272817	35.0	33.0	39.0	23.0	41.0
40	33.35073955330796	35.0	32.0	39.0	22.0	41.0
41	33.33825009439287	35.0	32.0	39.0	22.0	41.0
42	33.30257419542415	35.0	32.0	39.0	23.0	41.0
43	33.17325859961769	35.0	32.0	39.0	22.0	41.0
44	33.09982292292938	35.0	32.0	39.0	22.0	41.0
45	33.05001966792129	35.0	32.0	39.0	22.0	41.0
46	32.994773200582536	35.0	32.0	39.0	22.0	41.0
47	32.91567658307033	35.0	32.0	39.0	22.0	40.0
48	32.92763060091749	35.0	32.0	39.0	22.0	40.0
49	32.98797757199183	35.0	32.0	39.0	22.0	40.0
50	32.849001672102204	35.0	32.0	38.0	21.0	40.0
51	32.649856404440875	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	6.0
13	8.0
14	39.0
15	79.0
16	245.0
17	628.0
18	1532.0
19	2704.0
20	4376.0
21	6656.0
22	9787.0
23	13793.0
24	18908.0
25	26006.0
26	33419.0
27	38433.0
28	41480.0
29	45973.0
30	53636.0
31	63795.0
32	79165.0
33	100944.0
34	167018.0
35	226154.0
36	103137.0
37	117947.0
38	156992.0
39	207292.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98316978481058	23.595190765680726	26.926305154047842	16.495334295460854
2	30.331815592517508	26.515317955957013	30.141911616703133	13.01095483482235
3	28.61241828603604	24.887221902828628	32.62158261645185	13.878777194683478
4	26.043879855970296	27.28322201333735	31.725672623174468	14.947225507517883
5	22.68092843113136	32.018915409520325	31.52037636113198	13.779779798216337
6	20.68157569650095	40.50960307635232	28.664449475807142	10.14437175133959
7	85.0214636880181	4.998414726076506	8.327292628410476	1.6528289574949253
8	86.57700550307122	3.776569782968764	7.938407174647194	1.70801753931282
9	81.86959707730743	5.317377101803529	9.784823732011088	3.028202088877955
10	54.22202517757041	20.10857481900908	14.803893064393694	10.865506939026814
11	49.69938996554496	18.826015858001554	19.653252574261202	11.821341602192282
12	44.3512282912852	20.590734896154693	23.222026493150434	11.836010319409674
13	20.210663828522037	43.209765287368725	23.766347726217273	12.813223157891967
14	14.109661488105182	44.28505461630451	27.63382408853327	13.971459807057036
15	13.782410958255332	22.028729636465773	51.16461721225962	13.024242193019269
16	15.618565991467149	16.4337651505484	49.435945066640706	18.511723791343744
17	15.708420106798787	17.92010745657599	29.140557884863068	37.230914551762154
18	21.0224424795526	21.967884060564042	37.669265814258516	19.34040764562484
19	31.222594823718858	22.440440403567326	26.438816977823265	19.89814779489055
20	33.06749846406033	21.098614562681465	26.58760907802837	19.246277895229838
21	22.085891588312915	29.35144536198842	28.553085627156726	20.009577422541938
22	22.787161517705734	24.503730327145284	25.1116598541548	27.59744830099418
23	19.10241922009785	30.894554945857305	25.603357886441763	24.399667947603078
24	18.825226509989857	22.735853896945354	42.91152329694877	15.527396296116013
25	16.323322207911637	24.130697612616938	40.005538591882086	19.540441587589346
26	16.16084807550377	34.90056188422633	28.259185050801122	20.679404989468782
27	16.780617822688757	36.66087372931415	29.519773825483046	17.03873462251405
28	14.731601942322342	28.83448819332711	40.1579485371408	16.27596132720975
29	15.637378785745954	23.633013691241263	39.08390900922353	21.645698513789252
30	17.189302755745466	32.83266743058013	31.742314710421105	18.2357151032533
31	29.969439076147086	26.584649022984497	25.83483419087224	17.611077709996174
32	32.229934444647625	24.458869048480437	26.94840689837539	16.36278960849654
33	29.75769647200906	26.41697834949962	25.301892724974053	18.52343245351727
34	19.47860932667299	26.664965183174782	28.619390860139372	25.237034630012857
35	19.935181372439388	25.25558430828776	32.50423287871273	22.305001440560122
36	31.483408562584113	25.817402755613912	24.94056867261923	17.75862000918275
37	20.163960737829896	34.78235701947453	28.50953992851138	16.54414231418419
38	19.83868357800929	35.249585263398856	24.026635233074735	20.88509592551712
39	20.287756817664558	33.61780558621588	27.31920312687059	18.775234469248975
40	25.533434808405502	25.692883106768527	24.89551005695146	23.87817202787451
41	15.908454048763291	25.027660069909924	27.61422194624277	31.449663935084022
42	20.79925432924495	25.15132459174263	24.35592491195481	29.693496167057614
43	21.276612539319398	25.72090496118381	26.408032405367038	26.59445009412975
44	18.873508296705392	29.6255464590506	29.486029197982955	22.014916046261057
45	16.98893991877609	39.707165043460186	21.78034812878476	21.523546908978965
46	20.918708995015265	35.33529530166908	24.750598917803877	18.99539678551178
47	20.884306577505424	27.957785668334385	27.03503784265926	24.12286991150093
48	22.645802444610794	24.162403091086816	32.36201867860512	20.829775785697276
49	21.564987679593116	24.265676122617318	33.999060675866076	20.170275521923482
50	19.519721202282266	35.27412083076247	26.068810097339767	19.137347869615496
51	17.372826168465284	35.996637377470165	23.986049589473254	22.644486864591297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1157.0
1	2008.5
2	2860.0
3	9083.0
4	15306.0
5	11571.0
6	7836.0
7	8146.5
8	8457.0
9	9201.5
10	9946.0
11	10138.5
12	10331.0
13	10275.5
14	10220.0
15	9570.5
16	8921.0
17	8340.0
18	7759.0
19	7428.0
20	7097.0
21	6891.5
22	6686.0
23	6997.5
24	7309.0
25	7443.0
26	9320.0
27	11063.0
28	13472.5
29	15882.0
30	18559.0
31	21236.0
32	25258.5
33	29281.0
34	31353.0
35	33425.0
36	35697.0
37	37969.0
38	48915.0
39	59861.0
40	83992.5
41	108124.0
42	136469.5
43	164815.0
44	163470.0
45	162125.0
46	151440.0
47	140755.0
48	127106.5
49	113458.0
50	109084.0
51	104710.0
52	99787.0
53	94864.0
54	84908.0
55	74952.0
56	68229.0
57	61506.0
58	57111.5
59	52717.0
60	47907.5
61	43098.0
62	37213.5
63	31329.0
64	25729.0
65	20129.0
66	15822.0
67	11515.0
68	9105.5
69	6696.0
70	5405.0
71	4114.0
72	3153.5
73	2193.0
74	1831.0
75	1090.5
76	712.0
77	564.5
78	417.0
79	305.0
80	193.0
81	129.0
82	65.0
83	70.5
84	76.0
85	46.0
86	16.0
87	12.0
88	8.0
89	7.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1520242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.137435928002393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5715718865925	14.150650226809228
2	6.049422279600693	2.073431734361741
3	1.9731554399674418	1.0144447478529421
4	1.047235244226541	0.7178770759791313
5	0.6660279476207656	0.5707005639304903
6	0.4463678366850447	0.45897601209065936
7	0.33055860232244566	0.3965448807425655
8	0.2675569087135413	0.36681915041381596
9	0.21404118918953124	0.33013054491201277
>10	3.28197069237509	13.679184412504085
>50	1.4489672759347907	18.391432933437418
>100	1.693482912935744	45.817345554338466
>500	0.007327755715276506	0.7772014754101063
>1k	0.001542685413742422	0.3329163901386772
>5k	7.71342706871211E-4	0.9223442970786659
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8525	0.5607659833105518	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5430	0.3571799752934072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009209060136478271	0.0
2	0.0	0.0	0.0	0.03446819651081867	0.0
3	0.0	0.0	0.0	0.05091294675452987	0.0
4	0.0	0.0	0.0	0.08524958526339885	0.0
5	0.0	0.0	0.0	0.13938570306569611	0.0
6	0.0	0.0	0.0	0.21279506815362292	0.0
7	0.0	0.0	0.0	0.2565381038018947	0.0
8	0.0	0.0	0.0	0.3976340608929368	0.0
9	0.0	0.0	0.0	0.45808496278881916	0.0
10	0.0	0.0	0.0	0.5447159070726898	0.0
11	0.0	0.0	0.0	0.6157572281255221	0.0
12	0.0	0.0	0.0	0.6871932231842036	0.0
13	0.0	0.0	0.0	0.7187013646511542	0.0
14	0.0	0.0	0.0	0.7286339937983558	0.0
15	0.0	0.0	0.0	0.7469205560693627	0.0
16	0.0	0.0	0.0	0.7832963436084518	0.0
17	0.0	0.0	0.0	0.8232241972001826	0.0
18	0.0	0.0	0.0	0.8882138501633292	0.0
19	0.0	0.0	0.0	0.9139992185454684	0.0
20	0.0	0.0	0.0	0.9434682109821989	0.0
21	0.0	0.0	0.0	0.9810937995398101	0.0
22	0.0	0.0	0.0	1.0180615980876728	0.0
23	0.0	0.0	0.0	1.0625939817476429	0.0
24	0.0	0.0	0.0	1.0976541892672351	0.0
25	0.0	0.0	0.0	1.1267942866990914	0.0
26	0.0	0.0	0.0	1.156657953141671	0.0
27	0.0	0.0	0.0	1.1885607686144706	0.0
28	0.0	0.0	0.0	1.2178982030492513	0.0
29	0.0	0.0	0.0	1.2512481565434976	0.0
30	6.577900097484479E-5	0.0	0.0	1.3010428602814552	0.0
31	6.577900097484479E-5	0.0	0.0	1.345575243941425	0.0
32	6.577900097484479E-5	0.0	0.0	1.387213351558502	0.0
33	6.577900097484479E-5	0.0	0.0	1.428719901173629	0.0
34	6.577900097484479E-5	0.0	0.0	1.4751598758618694	0.0
35	6.577900097484479E-5	0.0	0.0	1.5380446007938209	0.0
36	6.577900097484479E-5	0.0	0.0	1.583761006471338	0.0
37	6.577900097484479E-5	0.0	0.0	1.6294774121488553	0.0
38	6.577900097484479E-5	0.0	0.0	1.6767725138497687	0.0
39	6.577900097484479E-5	0.0	0.0	1.7299219466374431	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.034005E-4	45.000004	1
CCGTAAG	40	6.8175723E-9	45.000004	1
TACGAGT	20	7.034005E-4	45.000004	38
TCGTATA	20	7.034005E-4	45.000004	22
CATCGTA	20	7.034005E-4	45.000004	35
ACCCGTA	20	7.034005E-4	45.000004	33
TTGCAAC	20	7.034005E-4	45.000004	27
GTCCAAT	20	7.034005E-4	45.000004	36
TCAACTT	20	7.034005E-4	45.000004	39
TACCCGC	25	3.8914026E-5	45.0	40
TAACGCG	35	1.2122837E-7	45.0	1
CATTACG	25	3.8914026E-5	45.0	35
CGTTAGT	25	3.8914026E-5	45.0	22
TCGCTCA	25	3.8914026E-5	45.0	40
TGTTACG	25	3.8914026E-5	45.0	1
TTACGAG	35	1.2122837E-7	45.0	1
TAGTGCG	110	0.0	45.0	1
AATACGC	25	3.8914026E-5	45.0	17
GTGCCGT	35	1.2122837E-7	45.0	41
CTTATCG	30	2.165967E-6	44.999996	27
>>END_MODULE