FastQCFastQC Report
Thu 26 May 2016
SRR1545444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545444_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1110608
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70000.6302853932260527No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31640.28488899773817583No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA15690.1412739688530967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT253.8907197E-545.00000434
ATTACGA207.0331834E-445.013
TTATACG207.0331834E-445.01
CGCCTAC207.0331834E-445.014
CTACGCG351.2118835E-745.01
GTGTCGA207.0331834E-445.07
TCGTACC207.0331834E-445.038
CACGTAA302.165436E-644.99999632
GTACGAG800.042.18751
CGTTTTT57500.041.6739121
CGGTCTA603.6379788E-1241.24999631
ATGTACG556.184564E-1140.9090921
GTCGACG451.9281288E-840.01
CGGGCTA451.9281288E-840.06
TACGGGA2050.039.5121964
CGCATCG403.4580808E-739.37521
TATTGCG800.039.3751
ATACTAT6050.039.04958345
GCGCGAC2250.039.0000049
ATGGTCC4700.038.77659611