FastQCFastQC Report
Thu 26 May 2016
SRR1545443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545443_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1261554
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89150.7066681251852873No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18820.14918108935487503No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14130.11200471799066865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG502.1827873E-1145.01
CGTTTTT68750.041.2036361
TCGGACG501.0822987E-940.51
TATACGG1000.040.52
GCGATCG1100.038.8636329
TCGCACG356.248776E-638.57142635
ATTACGC356.248776E-638.57142625
CGTCTAC356.248776E-638.57142620
CGATACG356.248776E-638.57142610
TAGGGCG4500.038.55
AATGCGG1700.038.382352
GGCGATA4650.038.2258078
GGGCGAT23700.037.7848137
TATGGGC4550.037.5824174
GGTACGA301.1399849E-437.5000048
CTATCGT301.1399849E-437.50000414
TACGGAC301.1399849E-437.5000044
ATGCGTC301.1399849E-437.50000416
CGACGGT1100.036.8181828
ATCGTAG552.750312E-936.818181
Produced by FastQC (version 0.11.5)