##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545443_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1261554
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.907682905369093	31.0	31.0	33.0	28.0	34.0
2	31.194873148513658	31.0	31.0	34.0	28.0	34.0
3	31.283358461072613	31.0	31.0	34.0	28.0	34.0
4	35.13526571197111	35.0	35.0	37.0	32.0	37.0
5	35.015467431437735	35.0	35.0	37.0	32.0	37.0
6	35.06766416657551	37.0	35.0	37.0	32.0	37.0
7	35.55384232462502	37.0	35.0	37.0	35.0	37.0
8	35.46331429332395	37.0	35.0	37.0	35.0	37.0
9	37.14670477839236	39.0	37.0	39.0	34.0	39.0
10	36.520345542085394	38.0	35.0	39.0	32.0	39.0
11	36.248353221503	38.0	35.0	39.0	31.0	39.0
12	35.74771591227962	37.0	35.0	39.0	30.0	39.0
13	35.442215711733304	37.0	35.0	39.0	30.0	39.0
14	36.455913896670296	38.0	35.0	40.0	30.0	41.0
15	36.70583344034421	38.0	35.0	40.0	31.0	41.0
16	36.72667361048358	38.0	35.0	40.0	31.0	41.0
17	36.634137738059565	38.0	35.0	40.0	31.0	41.0
18	36.55252093846161	38.0	35.0	40.0	31.0	41.0
19	36.477808322117006	38.0	35.0	40.0	31.0	41.0
20	36.35268961931079	38.0	35.0	40.0	30.0	41.0
21	36.20132313004437	38.0	34.0	40.0	30.0	41.0
22	36.08387195474788	38.0	34.0	40.0	30.0	41.0
23	36.00770161245575	38.0	34.0	40.0	30.0	41.0
24	35.90575591690883	38.0	34.0	40.0	30.0	41.0
25	35.751316233787854	37.0	34.0	40.0	29.0	41.0
26	35.618518113374456	37.0	34.0	40.0	29.0	41.0
27	35.519424455869505	37.0	34.0	40.0	29.0	41.0
28	35.53183058355013	37.0	34.0	40.0	29.0	41.0
29	35.51310526541076	37.0	34.0	40.0	29.0	41.0
30	35.39358124979192	37.0	34.0	40.0	28.0	41.0
31	35.25834803742052	37.0	34.0	40.0	27.0	41.0
32	35.05334611122473	37.0	34.0	40.0	26.0	41.0
33	34.81328345833789	37.0	33.0	40.0	25.0	41.0
34	34.62458444109408	37.0	33.0	40.0	24.0	41.0
35	34.43915520064936	37.0	33.0	40.0	24.0	41.0
36	34.27634964496169	37.0	33.0	40.0	23.0	41.0
37	34.224263884066794	37.0	33.0	40.0	23.0	41.0
38	34.14737696523494	37.0	33.0	40.0	23.0	41.0
39	34.06436823156203	36.0	33.0	40.0	23.0	41.0
40	33.91967684300474	36.0	33.0	40.0	23.0	41.0
41	33.81978575629739	36.0	33.0	40.0	22.0	41.0
42	33.774115099314024	36.0	33.0	40.0	22.0	41.0
43	33.66620533088556	36.0	32.0	40.0	22.0	41.0
44	33.59302970780482	36.0	32.0	40.0	22.0	41.0
45	33.54705545699986	35.0	32.0	40.0	22.0	41.0
46	33.47522737829693	35.0	32.0	40.0	22.0	41.0
47	33.36823948875752	35.0	32.0	40.0	22.0	41.0
48	33.30425411833342	35.0	32.0	39.0	21.0	41.0
49	33.27883784602165	35.0	32.0	39.0	21.0	41.0
50	33.22600300898733	35.0	32.0	39.0	21.0	41.0
51	33.04444676961906	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	8.0
14	22.0
15	66.0
16	169.0
17	499.0
18	1042.0
19	2016.0
20	3342.0
21	5055.0
22	7406.0
23	10436.0
24	14494.0
25	19648.0
26	25424.0
27	29631.0
28	31798.0
29	35790.0
30	41225.0
31	48888.0
32	59275.0
33	74144.0
34	110060.0
35	137743.0
36	103009.0
37	124780.0
38	165926.0
39	209586.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.76014502748198	22.73656141552403	25.846297502921	16.65699605407299
2	34.304199423885144	24.20403724295591	28.110885463483925	13.38087786967502
3	29.2272863468389	25.193531152848	32.14432358820946	13.434858912103644
4	25.94736333125653	27.669128709512236	31.04187375253061	15.341634206700624
5	23.085971745957764	31.282450057627337	31.006916866023964	14.624661330390932
6	21.357785715078386	40.38939276479643	28.450862983273012	9.801958536852167
7	84.01574565971809	4.793928757706765	9.198258655594609	1.9920669269805336
8	84.5770375267329	4.088053305684893	9.332141152895556	2.0027680146866484
9	79.53341672255011	5.493938428319359	11.268800225753317	3.7038446233771998
10	43.427391930904264	24.928302712369028	18.215629295297706	13.428676061428998
11	35.84737553842325	24.367090112670564	24.5392587237645	15.246275625141688
12	32.263224562721845	22.766841530366516	29.183451520901997	15.786482386009638
13	22.560112369347646	31.20183519690794	29.10584881820358	17.132203615540835
14	18.82899978914894	33.241700315642454	31.016270409352277	16.91302948585633
15	18.417523150019736	23.501411750904044	40.789137841107085	17.29192725796914
16	20.235519050314135	21.077734286443544	39.253333586988745	19.433413076253572
17	20.45580292242742	20.976589190791675	31.42196053438854	27.145647352392366
18	22.814560454804152	23.37157188673652	33.442642962568385	20.37122469589094
19	28.153689814308386	23.68705580577605	27.827504807562736	20.331749572352827
20	28.93463141490574	23.66113539333235	28.313492724053035	19.090740467708873
21	24.702311593479152	26.09075790651847	30.239371441888334	18.967559058114038
22	23.968692580737724	24.21140910337568	28.834199725100945	22.98569859078565
23	21.731055507730943	27.469216537698742	28.73368876797981	22.066039186590505
24	20.835731169652664	24.50136894655322	35.63375012088266	19.02914976291146
25	20.746951775350084	25.495777430058485	33.03750770874652	20.71976308584492
26	20.003345080749614	28.991149011457296	29.440832497063145	21.564673410729938
27	19.97013207520249	29.3635468636301	30.83672993783857	19.829591123328846
28	19.405510980901333	27.08310543979885	34.36634499989695	19.14503857940286
29	19.824993618981036	24.723793036207724	34.727566160465585	20.723647184345655
30	20.82407887415045	27.490856515059996	32.241505318044254	19.443559292745295
31	25.645671925260434	26.586654237551464	28.1167512448932	19.6509225922949
32	27.12242202870428	25.811261349098018	28.589422252238112	18.47689436995959
33	25.856840055994436	26.60837348222906	27.395418666184725	20.139367795591788
34	20.760982090342544	26.991630956740654	30.005057254782592	22.242329698134206
35	22.17582441972361	26.23740244175041	30.516252177869518	21.07052096065646
36	26.25341443965141	27.36299833380101	26.677811651344296	19.70577557520328
37	21.633715243263467	30.54780056977347	28.53694728881998	19.281536898143084
38	22.45460757129699	30.586720822097192	26.50936860411841	20.449303002487408
39	21.728360418975328	30.150988384167466	27.027856120308762	21.092795076548448
40	23.8737303357605	26.753432671134174	27.51289282900296	21.85994416410237
41	20.064618716281664	26.34052922031082	28.19681123439821	25.3980408290093
42	22.058508791538056	27.203036889423682	26.684707907866013	24.053746411172252
43	22.11161789348692	27.333510892121936	27.766310439347023	22.78856077504411
44	20.763439377149133	28.351541035897	28.945808106509908	21.93921148044396
45	19.768317487796796	32.44371624203165	26.246835252395062	21.54113101777649
46	21.561899054657985	30.675103879818067	27.35721181970807	20.405785245815874
47	21.78083538239346	28.073788359436058	28.45419221055936	21.69118404761112
48	22.777384083439948	26.992661431853094	29.752194515652917	20.47775996905404
49	21.655513755257406	27.293480897369438	30.165652837690658	20.8853525096825
50	20.474430741767694	31.158555242185432	28.18682355253917	20.180190463507707
51	20.33785315571113	30.498099962427293	27.14089131341187	22.023155568449706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	832.0
1	1618.0
2	2404.0
3	9502.0
4	16600.0
5	12189.0
6	7778.0
7	7827.0
8	7876.0
9	8102.0
10	8328.0
11	8193.5
12	8059.0
13	8067.5
14	8076.0
15	7680.5
16	7285.0
17	7100.0
18	6915.0
19	6662.5
20	6410.0
21	6570.0
22	6730.0
23	7156.5
24	7583.0
25	8942.5
26	11293.0
27	12284.0
28	14340.0
29	16396.0
30	20281.0
31	24166.0
32	26763.0
33	29360.0
34	32432.5
35	35505.0
36	37874.5
37	40244.0
38	45495.5
39	50747.0
40	60791.5
41	70836.0
42	83826.5
43	96817.0
44	97754.5
45	98692.0
46	95491.5
47	92291.0
48	90837.0
49	89383.0
50	85778.5
51	82174.0
52	80804.0
53	79434.0
54	72288.5
55	65143.0
56	64332.5
57	63522.0
58	59941.5
59	56361.0
60	52478.5
61	48596.0
62	41721.0
63	34846.0
64	29668.5
65	24491.0
66	20444.0
67	16397.0
68	13436.5
69	10476.0
70	8833.5
71	7191.0
72	6010.5
73	4830.0
74	3796.5
75	2213.5
76	1664.0
77	1268.5
78	873.0
79	674.0
80	475.0
81	369.5
82	264.0
83	186.0
84	108.0
85	65.5
86	23.0
87	17.5
88	12.0
89	10.5
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1261554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.009476226717965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15652777923225	16.761652876663984
2	5.76890762034833	2.5394127024837734
3	2.637789431335064	1.7416909134017098
4	1.570002166481934	1.382197014363193
5	1.1429527674783204	1.25778958820378
6	0.9198609158648273	1.2147394175768358
7	0.759745257276299	1.1705116624869099
8	0.6683271292288512	1.1767624049946452
9	0.5920732897376593	1.172810069546014
>10	7.953132927197966	42.22423211828576
>50	1.7344243419940997	25.157255240276704
>100	0.09372331135534993	3.0447420528067473
>500	0.0014474642680360065	0.183740613561019
>1k	7.237321340180032E-4	0.2624297016400336
>5k	3.618660670090016E-4	0.7100336237089225
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8915	0.7066681251852873	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1882	0.14918108935487503	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1413	0.11200471799066865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006341385307327312	0.0
2	0.0	0.0	0.0	0.022749719790036732	0.0
3	0.0	0.0	0.0	0.03400567871054271	0.0
4	0.0	0.0	0.0	0.048353062968370754	0.0
5	0.0	0.0	0.0	0.07704783148402684	0.0
6	0.0	0.0	0.0	0.13982754602656725	0.0
7	0.0	0.0	0.0	0.17510150179857542	0.0
8	0.0	0.0	0.0	0.27347224137849035	0.0
9	0.0	0.0	0.0	0.32563013553125747	0.0
10	0.0	0.0	0.0	0.40362917481138344	0.0
11	0.0	0.0	0.0	0.46720156251733974	0.0
12	0.0	0.0	0.0	0.5193594566701069	0.0
13	0.0	0.0	0.0	0.5467859481242975	0.0
14	0.0	0.0	0.0	0.5608162631167591	0.0
15	0.0	0.0	0.0	0.5731026971497059	0.0
16	0.0	0.0	0.0	0.6014803963999955	0.0
17	0.0	0.0	0.0	0.629699561017602	0.0
18	0.0	0.0	0.0	0.6791623664147551	0.0
19	0.0	0.0	0.0	0.7015157496230839	0.0
20	0.0	0.0	0.0	0.726326419638002	0.0
21	0.0	0.0	0.0	0.7574784749602474	0.0
22	0.0	0.0	0.0	0.7901366092929831	0.0
23	0.0	0.0	0.0	0.8269166440754815	0.0
24	0.0	0.0	0.0	0.858623570612118	0.0
25	0.0	0.0	0.0	0.8880317449748485	0.0
26	0.0	0.0	0.0	0.9168057808068462	0.0
27	0.0	0.0	0.0	0.9489090439251907	0.0
28	0.0	0.0	0.0	0.9766526046447477	0.0
29	0.0	0.0	0.0	1.011292421886023	0.0
30	0.0	0.0	0.0	1.0629747121407407	0.0
31	0.0	0.0	0.0	1.1058583302815417	0.0
32	0.0	0.0	0.0	1.1496138889021001	0.0
33	0.0	0.0	0.0	1.1925767743592426	0.0
34	0.0	0.0	0.0	1.2398200948988312	0.0
35	0.0	0.0	0.0	1.293166998796722	0.0
36	0.0	0.0	0.0	1.345166358316806	0.0
37	0.0	0.0	0.0	1.3952633022446919	0.0
38	0.0	0.0	0.0	1.4501162851530731	0.0
39	7.92673163415914E-5	0.0	0.0	1.5347737790058926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	50	2.1827873E-11	45.0	1
CGTTTTT	6875	0.0	41.203636	1
TCGGACG	50	1.0822987E-9	40.5	1
TATACGG	100	0.0	40.5	2
GCGATCG	110	0.0	38.863632	9
TCGCACG	35	6.248776E-6	38.571426	35
ATTACGC	35	6.248776E-6	38.571426	25
CGTCTAC	35	6.248776E-6	38.571426	20
CGATACG	35	6.248776E-6	38.571426	10
TAGGGCG	450	0.0	38.5	5
AATGCGG	170	0.0	38.38235	2
GGCGATA	465	0.0	38.225807	8
GGGCGAT	2370	0.0	37.784813	7
TATGGGC	455	0.0	37.582417	4
GGTACGA	30	1.1399849E-4	37.500004	8
CTATCGT	30	1.1399849E-4	37.500004	14
TACGGAC	30	1.1399849E-4	37.500004	4
ATGCGTC	30	1.1399849E-4	37.500004	16
CGACGGT	110	0.0	36.81818	28
ATCGTAG	55	2.750312E-9	36.81818	1
>>END_MODULE