FastQCFastQC Report
Thu 26 May 2016
SRR1545442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545442_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1493043
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113260.7585849838216313No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29370.1967123518880568No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23150.15505246667376626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA207.033966E-445.026
ACGTATA207.033966E-445.042
GCGATCG1000.042.759
CGTTTTT87850.041.286281
TAATGCG556.184564E-1140.9090921
GGGCGAT29250.039.6923077
TACGGGC1600.039.3754
TATCGCC403.459154E-739.37513
TACGGGA3900.039.230774
ATAGGGC6150.039.146344
GCGATAT1400.038.571439
TTATTCG356.2495437E-638.571431
TAGGGCG5900.038.516955
TAGCATA7950.038.49056630
TAAGGGC6550.038.473284
CGTATGG1350.038.3333362
TAGCCGT1350.038.33333644
AGGGCGA16550.038.202426
AGGGCTT8650.037.976886
CGGTCTA950.037.89473731
Produced by FastQC (version 0.11.5)