##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545442_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1493043
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87250601623664	31.0	31.0	33.0	28.0	34.0
2	31.166012633259726	31.0	31.0	34.0	28.0	34.0
3	31.260788872122237	31.0	31.0	34.0	28.0	34.0
4	35.113323594832835	35.0	35.0	37.0	32.0	37.0
5	34.971726199446366	35.0	35.0	37.0	32.0	37.0
6	35.021144735952014	37.0	35.0	37.0	32.0	37.0
7	35.50762704088228	37.0	35.0	37.0	35.0	37.0
8	35.416380506120724	37.0	35.0	37.0	35.0	37.0
9	37.087554075803574	39.0	37.0	39.0	33.0	39.0
10	36.48416489009359	38.0	35.0	39.0	32.0	39.0
11	36.1956320079194	38.0	35.0	39.0	31.0	39.0
12	35.630128536150664	37.0	35.0	39.0	30.0	39.0
13	35.261486105892466	37.0	35.0	39.0	30.0	39.0
14	36.3065176287622	38.0	35.0	40.0	30.0	41.0
15	36.58390079857044	38.0	35.0	40.0	31.0	41.0
16	36.62231429369415	38.0	35.0	40.0	31.0	41.0
17	36.53361691525294	38.0	35.0	40.0	31.0	41.0
18	36.458250030307234	38.0	35.0	40.0	31.0	41.0
19	36.36641811387884	38.0	35.0	40.0	30.0	41.0
20	36.21126786033624	38.0	34.0	40.0	30.0	41.0
21	36.05193018553384	38.0	34.0	40.0	30.0	41.0
22	35.95031355426468	37.0	34.0	40.0	30.0	41.0
23	35.86254113243892	37.0	34.0	40.0	30.0	41.0
24	35.742200994880925	37.0	34.0	40.0	29.0	41.0
25	35.619094694526545	37.0	34.0	40.0	29.0	41.0
26	35.446456666016985	37.0	34.0	40.0	29.0	41.0
27	35.36770809681972	37.0	34.0	40.0	29.0	41.0
28	35.37423235633535	37.0	34.0	40.0	28.0	41.0
29	35.376568524818104	37.0	34.0	40.0	29.0	41.0
30	35.23349695889536	37.0	34.0	40.0	27.0	41.0
31	35.098853817338146	37.0	34.0	40.0	27.0	41.0
32	34.89532853373948	37.0	34.0	40.0	26.0	41.0
33	34.62910914153176	37.0	33.0	40.0	25.0	41.0
34	34.43591845646777	36.0	33.0	40.0	24.0	41.0
35	34.25193246276229	36.0	33.0	40.0	23.0	41.0
36	34.0650617564263	36.0	33.0	40.0	23.0	41.0
37	34.02296718848687	36.0	33.0	40.0	23.0	41.0
38	33.973608261784825	36.0	33.0	40.0	23.0	41.0
39	33.900899036397476	36.0	33.0	40.0	23.0	41.0
40	33.73976369066396	36.0	33.0	40.0	22.0	41.0
41	33.659475313169146	36.0	33.0	40.0	22.0	41.0
42	33.611574482449605	36.0	32.0	40.0	22.0	41.0
43	33.53089093884101	35.0	32.0	40.0	21.0	41.0
44	33.45349129261515	35.0	32.0	40.0	21.0	41.0
45	33.40590525524047	35.0	32.0	40.0	21.0	41.0
46	33.32423178702824	35.0	32.0	40.0	21.0	41.0
47	33.22701891372184	35.0	32.0	39.0	21.0	41.0
48	33.16799851042468	35.0	32.0	39.0	20.0	41.0
49	33.17103593131611	35.0	32.0	39.0	20.0	41.0
50	33.101382210693195	35.0	32.0	39.0	20.0	41.0
51	32.92722781594368	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	4.0
13	13.0
14	22.0
15	79.0
16	221.0
17	644.0
18	1477.0
19	2567.0
20	4210.0
21	6504.0
22	9491.0
23	13056.0
24	18058.0
25	24452.0
26	31497.0
27	36044.0
28	39116.0
29	43084.0
30	49523.0
31	59576.0
32	71078.0
33	89924.0
34	134504.0
35	170255.0
36	118190.0
37	140338.0
38	185389.0
39	243634.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.01800417000716	22.850447040038365	26.895608498884492	17.23594029106998
2	33.460054398969085	25.287483347766944	28.28706206050328	12.96540019276069
3	28.679816991205207	25.15379664215967	32.72176353929525	13.44462282733987
4	26.86506684670167	27.845748581922958	30.766026162675825	14.52315840869955
5	23.02612851739702	31.997203027642207	30.582240431119533	14.394428023841243
6	21.7458572860929	40.087325013412205	28.12336952117253	10.043448179322363
7	83.35674190227608	5.029928809819944	9.422836448782789	2.1904928391211773
8	83.69718755588418	4.763225171679583	9.4257834503092	2.113803822127025
9	78.26117533118604	6.728272394030179	11.415344367174958	3.595207907608823
10	44.496642092692575	25.46195923359207	17.67135976659748	12.370038907117879
11	37.84251357797464	23.4999259900753	24.063071190849826	14.594489241100222
12	35.43320587551732	21.644922483813257	28.10990708238142	14.811964558288008
13	22.157164930949744	33.22529893646733	27.73744627582729	16.880089856755635
14	17.346854712154975	35.272728246942656	29.610332723170064	17.77008431773231
15	16.851088682643432	24.151481236642212	41.78339136917021	17.21403871154414
16	18.33476999657746	20.95123851088013	40.707802789336945	20.006188703205467
17	18.15915549652622	21.744249830714857	31.158312252225823	28.938282420533103
18	21.342519940818853	24.08055226808605	34.06218039266116	20.514747398433936
19	28.38163401857817	24.284029328023372	27.26324693930449	20.07108971409397
20	30.187543158502468	22.486358396911545	28.027926858101203	19.298171586484784
21	23.658193367505156	26.79949606273898	28.64900742979271	20.893303139963148
22	23.46275358445805	25.289224757759825	26.768083705559718	24.479937952222407
23	21.358661471906704	28.84645653206237	27.53785389972024	22.257028096310687
24	20.26873974828588	23.888394373102447	37.39537307364892	18.44749280496275
25	19.97651775601908	25.75739613661495	33.483764365795224	20.78232174157074
26	18.69242881819211	31.093143332107648	28.850877034351992	21.36355081534825
27	19.40185245836858	31.264069420639593	29.120661628633606	20.213416492358224
28	17.50324672497711	28.57178259433921	34.58935877935197	19.33561190133171
29	18.746211596049143	25.07643785209133	34.46491494216845	21.71243560969108
30	19.19073998538555	30.058410909799647	31.535461470299246	19.215387634515547
31	26.12617319126107	27.551383315818768	27.177984826960778	19.144458665959384
32	27.307384984893268	26.53855247303661	27.479449687651325	18.674612854418797
33	25.60441996647116	28.63802315137608	26.197571000969162	19.559985881183596
34	20.472417740145463	27.491572580294072	28.72743785677974	23.308571822780724
35	21.632732613863098	27.152734382064015	30.152246117492933	21.062286886579958
36	25.914792809048365	29.24021612237558	25.492099021930382	19.352892046645675
37	21.63655032038595	32.47495216145818	28.037705544984306	17.85079197317157
38	20.577572112792463	31.67624777049288	27.179257395801727	20.566922720912928
39	21.555976619561527	31.540350813740798	26.83244889798887	20.071223668708804
40	23.48820496127707	27.639927316225986	27.6087828682764	21.26308485422054
41	18.387548114823215	27.13826728366162	29.372764213756735	25.101420387758424
42	21.29295673332918	27.819761386644593	25.775212100388266	25.112069779637963
43	21.74277632995165	27.48721905531187	27.24777518129083	23.522229433445656
44	20.072027396397825	30.091966540816305	28.38679127124939	21.44921479153648
45	18.372143334116966	34.26773374912846	25.261228243258905	22.09889467349567
46	20.271418840582623	33.23869439795103	25.978086364558823	20.511800396907525
47	21.786780420925584	28.996820587216842	27.327344222503974	21.889054769353596
48	21.93392956532397	26.471508188310715	30.391422082284304	21.20314016408101
49	20.63477073332784	28.11586806274166	30.005096973094545	21.24426423083595
50	18.99951977270581	32.029753999047585	28.43715820642808	20.533568021818528
51	19.05738816631537	32.015219923337774	27.048316759798613	21.879075150548243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1207.0
1	2064.0
2	2921.0
3	11929.5
4	20938.0
5	15341.5
6	9745.0
7	9816.0
8	9887.0
9	10078.5
10	10270.0
11	10278.0
12	10286.0
13	9890.5
14	9495.0
15	9373.0
16	9251.0
17	8771.5
18	8292.0
19	8277.0
20	8262.0
21	7972.0
22	7682.0
23	7822.0
24	7962.0
25	9499.0
26	12489.0
27	13942.0
28	17721.0
29	21500.0
30	25540.0
31	29580.0
32	32922.5
33	36265.0
34	42770.0
35	49275.0
36	49403.5
37	49532.0
38	57902.5
39	66273.0
40	77398.5
41	88524.0
42	104024.0
43	119524.0
44	120670.0
45	121816.0
46	115900.5
47	109985.0
48	102967.0
49	95949.0
50	95956.5
51	95964.0
52	89582.5
53	83201.0
54	78505.0
55	73809.0
56	72951.5
57	72094.0
58	69425.0
59	66756.0
60	61696.5
61	56637.0
62	49047.5
63	41458.0
64	34445.5
65	27433.0
66	22188.5
67	16944.0
68	14127.0
69	11310.0
70	9259.0
71	7208.0
72	5854.5
73	4501.0
74	3706.5
75	2253.5
76	1595.0
77	1273.0
78	951.0
79	717.5
80	484.0
81	350.0
82	216.0
83	158.5
84	101.0
85	71.0
86	41.0
87	26.0
88	11.0
89	8.5
90	6.0
91	5.5
92	5.0
93	5.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1493043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.295862718537922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3776272008257	15.907415618727022
2	5.575672142838458	2.26326152749251
3	2.330561903109151	1.4190229332767341
4	1.4206703779303906	1.1533492383507438
5	0.9851026838713752	0.9996754417758346
6	0.7289757201788747	0.8877114685138654
7	0.6265122772297821	0.8900925019093961
8	0.5216056504170115	0.8469149339261878
9	0.4548726996891361	0.8308830480571289
>10	6.353324203757812	30.818170290336084
>50	2.2145232251814506	31.54150854703282
>100	0.4049051653312982	10.533877701778046
>500	0.003321617435033366	0.4818156586999208
>1k	0.0019929704610200196	0.6627577641918178
>5k	0.0	0.0
>10k+	3.3216174350333657E-4	0.7635433259319019
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11326	0.7585849838216313	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2937	0.1967123518880568	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2315	0.15505246667376626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00642982151217346	0.0
2	0.0	0.0	0.0	0.02270530721486253	0.0
3	0.0	0.0	0.0	0.03456029062793235	0.0
4	0.0	0.0	0.0	0.05204136786415395	0.0
5	0.0	0.0	0.0	0.08954866001849913	0.0
6	0.0	0.0	0.0	0.15110080553607633	0.0
7	0.0	0.0	0.0	0.19028253037588336	0.0
8	0.0	0.0	0.0	0.29637458532674543	0.0
9	0.0	0.0	0.0	0.34392847359386164	0.0
10	6.697730741847355E-5	0.0	0.0	0.40802575679334085	0.0
11	6.697730741847355E-5	0.0	0.0	0.46026805657975023	0.0
12	6.697730741847355E-5	0.0	0.0	0.5103001052213499	0.0
13	6.697730741847355E-5	0.0	0.0	0.5316658662878431	0.0
14	6.697730741847355E-5	0.0	0.0	0.5424492127822172	0.0
15	6.697730741847355E-5	0.0	0.0	0.5546390827323795	0.0
16	6.697730741847355E-5	0.0	0.0	0.5800904595513994	0.0
17	6.697730741847355E-5	0.0	0.0	0.6103642025045495	0.0
18	6.697730741847355E-5	0.0	0.0	0.6538324750191388	0.0
19	6.697730741847355E-5	0.0	0.0	0.6737917126298438	0.0
20	6.697730741847355E-5	0.0	0.0	0.6989751802191899	0.0
21	6.697730741847355E-5	0.0	0.0	0.7280433316388074	0.0
22	1.339546148369471E-4	0.0	0.0	0.7607282576590225	0.0
23	1.339546148369471E-4	0.0	0.0	0.7970299582798352	0.0
24	1.339546148369471E-4	0.0	0.0	0.826700905466219	0.0
25	1.339546148369471E-4	0.0	0.0	0.8518843730555651	0.0
26	1.339546148369471E-4	0.0	0.0	0.8769338860300742	0.0
27	1.339546148369471E-4	0.0	0.0	0.9029210813084418	0.0
28	1.339546148369471E-4	0.0	0.0	0.9325920284948257	0.0
29	1.339546148369471E-4	0.0	0.0	0.9642053175963452	0.0
30	1.339546148369471E-4	0.0	0.0	1.0097498866409071	0.0
31	1.339546148369471E-4	0.0	0.0	1.045381814187535	0.0
32	1.339546148369471E-4	0.0	0.0	1.0828221290344617	0.0
33	1.339546148369471E-4	0.0	0.0	1.123142468100383	0.0
34	1.339546148369471E-4	0.0	0.0	1.1631279206292116	0.0
35	1.339546148369471E-4	0.0	0.0	1.2128920600411375	0.0
36	1.339546148369471E-4	0.0	0.0	1.2587715156227919	0.0
37	1.339546148369471E-4	0.0	0.0	1.3039811981302616	0.0
38	2.0093192225542064E-4	0.0	0.0	1.349458789867405	0.0
39	2.679092296738942E-4	0.0	0.0	1.4012992258093036	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	20	7.033966E-4	45.0	26
ACGTATA	20	7.033966E-4	45.0	42
GCGATCG	100	0.0	42.75	9
CGTTTTT	8785	0.0	41.28628	1
TAATGCG	55	6.184564E-11	40.909092	1
GGGCGAT	2925	0.0	39.692307	7
TACGGGC	160	0.0	39.375	4
TATCGCC	40	3.459154E-7	39.375	13
TACGGGA	390	0.0	39.23077	4
ATAGGGC	615	0.0	39.14634	4
GCGATAT	140	0.0	38.57143	9
TTATTCG	35	6.2495437E-6	38.57143	1
TAGGGCG	590	0.0	38.51695	5
TAGCATA	795	0.0	38.490566	30
TAAGGGC	655	0.0	38.47328	4
CGTATGG	135	0.0	38.333336	2
TAGCCGT	135	0.0	38.333336	44
AGGGCGA	1655	0.0	38.20242	6
AGGGCTT	865	0.0	37.97688	6
CGGTCTA	95	0.0	37.894737	31
>>END_MODULE