##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545441_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1386554
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.048376767150792	31.0	31.0	33.0	30.0	34.0
2	31.33574530815244	31.0	31.0	34.0	30.0	34.0
3	31.411300966280432	31.0	31.0	34.0	30.0	34.0
4	35.225548373882305	37.0	35.0	37.0	33.0	37.0
5	35.102544870232244	37.0	35.0	37.0	32.0	37.0
6	35.14563803501342	37.0	35.0	37.0	32.0	37.0
7	35.659217022921574	37.0	35.0	37.0	35.0	37.0
8	35.62273881868286	37.0	35.0	37.0	35.0	37.0
9	37.390444223593164	39.0	37.0	39.0	35.0	39.0
10	36.802151953692395	39.0	37.0	39.0	32.0	39.0
11	36.38972228993606	38.0	35.0	39.0	32.0	39.0
12	34.942215737721	35.0	34.0	39.0	30.0	39.0
13	34.167635735788146	35.0	33.0	39.0	25.0	39.0
14	35.02702960000116	35.0	33.0	40.0	25.0	41.0
15	35.627021378179286	35.0	33.0	40.0	30.0	41.0
16	35.89610862613357	35.0	34.0	40.0	31.0	41.0
17	35.86559989729935	35.0	34.0	40.0	31.0	41.0
18	35.825473800515525	36.0	34.0	40.0	31.0	41.0
19	35.6810452387718	36.0	34.0	40.0	30.0	41.0
20	35.47625191662207	35.0	34.0	40.0	30.0	41.0
21	35.25729975175868	35.0	34.0	40.0	30.0	41.0
22	35.11700373732289	35.0	34.0	40.0	29.0	41.0
23	35.160025502072045	35.0	34.0	39.0	30.0	41.0
24	35.077269258896514	35.0	34.0	39.0	29.0	41.0
25	34.95788263565645	35.0	34.0	39.0	29.0	41.0
26	34.803067893497115	35.0	34.0	39.0	29.0	41.0
27	34.647020599269844	35.0	34.0	39.0	29.0	41.0
28	34.7923636583934	35.0	34.0	39.0	29.0	41.0
29	34.933747982408185	35.0	34.0	39.0	29.0	41.0
30	34.86074974360898	35.0	34.0	39.0	29.0	41.0
31	34.56128070021074	35.0	34.0	39.0	29.0	41.0
32	34.25511592047623	35.0	33.0	39.0	27.0	41.0
33	33.97857494190634	35.0	33.0	39.0	25.0	41.0
34	33.81375337707727	35.0	33.0	39.0	24.0	41.0
35	33.599794887180735	35.0	33.0	39.0	23.0	41.0
36	33.33079634835715	35.0	33.0	39.0	22.0	41.0
37	33.241856429681064	35.0	32.0	39.0	23.0	41.0
38	33.32316808432993	35.0	32.0	39.0	23.0	41.0
39	33.34122724394434	35.0	33.0	39.0	23.0	41.0
40	33.08987388879193	35.0	32.0	39.0	22.0	41.0
41	33.153964432687076	35.0	32.0	38.0	23.0	41.0
42	33.12656773555159	35.0	32.0	38.0	23.0	41.0
43	32.962709710548594	35.0	32.0	38.0	23.0	41.0
44	32.910736978148705	35.0	32.0	38.0	23.0	40.0
45	32.86245396861572	35.0	32.0	38.0	23.0	40.0
46	32.833780725453174	35.0	32.0	38.0	23.0	40.0
47	32.78011458623321	35.0	32.0	38.0	23.0	40.0
48	32.836280447786386	35.0	32.0	38.0	23.0	40.0
49	32.91285590031113	35.0	32.0	38.0	23.0	40.0
50	32.733246595516654	35.0	32.0	38.0	22.0	40.0
51	32.54780123961995	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	17.0
15	48.0
16	146.0
17	442.0
18	991.0
19	2015.0
20	3450.0
21	5524.0
22	8433.0
23	12058.0
24	17114.0
25	23643.0
26	31113.0
27	35348.0
28	37766.0
29	41356.0
30	48228.0
31	58842.0
32	73644.0
33	96585.0
34	172978.0
35	249867.0
36	81073.0
37	89350.0
38	122455.0
39	173973.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.366722103863246	23.047497609180745	29.99991345450664	16.585866832449366
2	29.651495722488992	25.879626758135636	31.223450366880773	13.245427152494601
3	26.483497938053617	25.06292578579702	34.2791553736818	14.174420902467554
4	24.106309599193395	27.559114178026967	33.985333423725294	14.349242799054347
5	22.478100384117745	32.12662471133471	31.713802708008487	13.681472196539046
6	20.164450861632506	38.72030948668426	30.66068829630869	10.454551355374548
7	85.46237651039917	5.011272550510114	8.093734539008217	1.4326164000825066
8	88.34059113456814	3.103665634371254	7.446879097388201	1.1088641336723994
9	85.02669207257705	4.153390347581126	8.955583410382863	1.8643341694589608
10	62.806785743649364	15.992525354223492	13.574372148506297	7.626316753620848
11	58.94599128486882	15.595497903435424	17.296477454177765	8.162033357517991
12	50.91817556330298	19.711313082649504	20.270108484775925	9.10040286927159
13	18.62379683733919	51.51822431726424	20.292610313049476	9.5653685323471
14	11.541562751973599	52.06735547263215	26.760948365516235	9.630133409878013
15	9.90325656267264	20.041339897328196	60.191092449338434	9.864311090660731
16	11.261948687176988	13.935627462038983	57.52989064976914	17.272533201014888
17	11.642099766759896	13.646998241684061	30.2723153948566	44.438586596699444
18	19.816682220815057	20.57583044006941	40.182495596998024	19.42499174211751
19	32.08522711701095	20.20152118128829	27.177520673554728	20.53573102814604
20	34.1396728868836	20.172384198523822	26.168328099735028	19.51961481485755
21	20.03715686514914	29.15364277193676	30.574936136638026	20.23426422627608
22	21.959260151425767	23.77498460211431	25.503153862020522	28.762601384439407
23	15.961152612880566	32.54564914168507	25.27164466728306	26.2215535781513
24	17.053861587792472	20.611314092346927	48.407707164668665	13.927117155191937
25	12.614510505901682	22.67571259395595	44.7385388524356	19.97123804770676
26	13.400055100630773	37.9624594498303	29.301058595626277	19.33642685391265
27	13.988925061699724	39.82578392186673	30.363765132840122	15.821525883593427
28	11.101767403216895	29.829490953832305	44.83280131895332	14.235940323997479
29	10.980747955002112	23.52616630870489	43.81091540610751	21.682170330185482
30	15.046943718023243	33.81469455931756	34.453544542801794	16.684817179857404
31	32.16073805996737	25.863832205597475	25.98723165487965	15.988198079555502
32	33.69064601883519	24.564640107777986	28.256238127040127	13.488475746346698
33	31.688343908711815	26.22357297299636	24.043852601485412	18.044230516806415
34	18.629350173163107	26.715872587724675	28.78149715048963	25.873280088622586
35	18.971421235667705	25.09646216447394	34.10166499104975	21.830451608808602
36	35.87245790643567	22.044074734918365	25.83483946532194	16.248627893324024
37	18.639519268632885	36.316508408615896	29.73652666971499	15.307445653036233
38	19.424919620873045	37.235188820630135	22.35535002603577	20.98454153246105
39	19.921113782802543	35.00938297390509	27.233631001749664	17.835872241542702
40	25.151995522713143	24.527641909366675	24.04551139010814	26.274851177812042
41	14.389991302177918	23.59042633752454	27.56784084860741	34.45174151169013
42	21.23473012951533	23.524651762571093	24.19992297451091	31.040695133402668
43	21.593749684469554	24.961667558566056	25.98614983621265	27.458432920751736
44	17.795844950863795	31.725919077078856	30.3418402745223	20.13639569753504
45	15.268211696046457	44.494841167383306	20.528807388677254	19.70813974789298
46	21.637671522349653	36.42259875922611	24.63892498957848	17.300804728845755
47	21.544418753254472	27.792426403876085	25.588906021691187	25.07424882117826
48	23.72594215587709	23.198086767626794	33.440818028003235	19.63515304849288
49	21.507492676087626	22.41874459992182	35.842094862515275	20.231667861475284
50	18.816937530020468	38.52017303328972	24.405324278751493	18.257565157938313
51	16.737682052051344	38.27640322699296	23.02398608348467	21.961928637471026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1127.0
1	1451.5
2	1776.0
3	7073.0
4	12370.0
5	9450.5
6	6531.0
7	7389.5
8	8248.0
9	9312.5
10	10377.0
11	10657.0
12	10937.0
13	10626.5
14	10316.0
15	9620.0
16	8924.0
17	8188.5
18	7453.0
19	7295.5
20	7138.0
21	6529.5
22	5921.0
23	6378.5
24	6836.0
25	6811.0
26	8499.0
27	10212.0
28	9849.0
29	9486.0
30	12124.5
31	14763.0
32	17776.0
33	20789.0
34	24373.0
35	27957.0
36	30219.5
37	32482.0
38	42522.0
39	52562.0
40	86086.5
41	119611.0
42	149811.0
43	180011.0
44	176507.0
45	173003.0
46	159825.5
47	146648.0
48	127354.0
49	108060.0
50	106904.5
51	105749.0
52	91992.0
53	78235.0
54	69115.0
55	59995.0
56	52261.5
57	44528.0
58	39720.0
59	34912.0
60	30545.5
61	26179.0
62	21434.5
63	16690.0
64	12635.5
65	8581.0
66	7033.0
67	5485.0
68	4114.0
69	2743.0
70	2084.5
71	1426.0
72	1081.0
73	736.0
74	559.0
75	318.5
76	255.0
77	206.5
78	158.0
79	122.0
80	86.0
81	63.0
82	40.0
83	39.0
84	38.0
85	23.5
86	9.0
87	6.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1386554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.466461782803004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.80880072177287	12.96225613425853
2	6.24096317853846	1.9305123697749171
3	1.8868653863681681	0.875493941626713
4	0.9053285290067656	0.560089163790576
5	0.49775823657369794	0.3849279371521258
6	0.36159162319725247	0.3355525812697206
7	0.2539209156960952	0.27490806969276
8	0.2205316797502866	0.2728675837404127
9	0.1821280889115079	0.2535189414052306
>10	2.3699319757961983	9.14517182337422
>50	1.1397374503611817	12.93007423232207
>100	2.1197992759997075	57.93489605704407
>500	0.011706424099843757	1.0924252796091434
>1k	0.0	0.0
>5k	9.365139279875007E-4	1.0473058849395267
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7272	0.5244656897603699	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7189	0.5184796264696506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011539399114639602	0.0
2	0.0	0.0	0.0	0.04183032179056856	0.0
3	0.0	0.0	0.0	0.060509724107391416	0.0
4	0.0	0.0	0.0	0.10313337958709146	0.0
5	0.0	0.0	0.0	0.15830613160396206	0.0
6	0.0	0.0	0.0	0.24030798656236974	0.0
7	0.0	0.0	0.0	0.2872589167100596	0.0
8	0.0	0.0	0.0	0.43416989168831505	0.0
9	0.0	0.0	0.0	0.495328706995905	0.0
10	0.0	0.0	0.0	0.5940626906705401	0.0
11	0.0	0.0	0.0	0.6755596969176823	0.0
12	0.0	0.0	0.0	0.7526573072523681	0.0
13	0.0	0.0	0.0	0.7805682288609026	0.0
14	0.0	0.0	0.0	0.7890785357079494	0.0
15	0.0	0.0	0.0	0.8033585421123158	0.0
16	0.0	0.0	0.0	0.8377603757228351	0.0
17	0.0	0.0	0.0	0.8798791824912697	0.0
18	0.0	0.0	0.0	0.9492598196680403	0.0
19	0.0	0.0	0.0	0.974430133986848	0.0
20	0.0	0.0	0.0	1.0054422691074418	0.0
21	7.212124446649752E-5	0.0	0.0	1.042440467518755	0.0
22	7.212124446649752E-5	0.0	0.0	1.0797992721524008	0.0
23	7.212124446649752E-5	0.0	0.0	1.1248750499439617	0.0
24	7.212124446649752E-5	0.0	0.0	1.1567526399981536	0.0
25	7.212124446649752E-5	0.0	0.0	1.1837981066730903	0.0
26	7.212124446649752E-5	0.0	0.0	1.2101223609033618	0.0
27	7.212124446649752E-5	0.0	0.0	1.2382496462452959	0.0
28	7.212124446649752E-5	0.0	0.0	1.264790264208967	0.0
29	7.212124446649752E-5	0.0	0.0	1.2941436107068314	0.0
30	7.212124446649752E-5	0.0	0.0	1.3470806041452406	0.0
31	7.212124446649752E-5	0.0	0.0	1.3910024420253377	0.0
32	7.212124446649752E-5	0.0	0.0	1.4273515492364524	0.0
33	7.212124446649752E-5	0.0	0.0	1.462258231558237	0.0
34	7.212124446649752E-5	0.0	0.0	1.5072618881053317	0.0
35	7.212124446649752E-5	0.0	0.0	1.5723873718585788	0.0
36	7.212124446649752E-5	0.0	0.0	1.6184728470726708	0.0
37	7.212124446649752E-5	0.0	0.0	1.6629716549084999	0.0
38	7.212124446649752E-5	0.0	0.0	1.705451067899267	0.0
39	7.212124446649752E-5	0.0	0.0	1.7478583596455675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTACG	115	0.0	45.000004	1
TGATCGC	20	7.033791E-4	45.0	40
CTATGCG	20	7.033791E-4	45.0	1
AAGTACG	20	7.033791E-4	45.0	44
TAATACG	105	0.0	45.0	1
GGTACGA	35	1.2121745E-7	45.0	8
ACACGGT	40	6.8175723E-9	45.0	3
TCACCGT	20	7.033791E-4	45.0	13
GATCGAC	20	7.033791E-4	45.0	16
GTACAAC	20	7.033791E-4	45.0	40
GCGGTAC	20	7.033791E-4	45.0	17
TAGACCG	35	1.2121745E-7	45.0	1
GTTTACG	40	6.8175723E-9	45.0	1
GCAACGA	20	7.033791E-4	45.0	11
ATAGCCG	45	3.8562575E-10	45.0	2
CGACACT	20	7.033791E-4	45.0	42
TATGCGT	20	7.033791E-4	45.0	21
CGTTCGA	20	7.033791E-4	45.0	14
TCGATAT	20	7.033791E-4	45.0	17
TCGATAG	90	0.0	45.0	1
>>END_MODULE