FastQCFastQC Report
Thu 26 May 2016
SRR1545440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545440_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1197847
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.6116807906185013No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31100.25963249062693317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA302.1655778E-645.00000437
CTATACG302.1655778E-645.0000041
TATCGTG302.1655778E-645.0000041
GTCGAAC253.8909046E-545.031
GGTCGAA253.8909046E-545.030
CCTGTCG207.033405E-445.08
TAGCCGT502.1827873E-1145.044
TCGTATA207.033405E-445.042
TCCGTAG453.8562575E-1045.01
CGCAACA207.033405E-445.044
CAATCCA207.033405E-445.028
ATACCGG207.033405E-445.02
TGCGTAG253.8909046E-545.01
CTTACGA207.033405E-445.034
TATGACG650.044.9999961
CGGTAGT1100.042.95454412
ATACTAT6150.041.34146545
GCGATAT1700.041.029419
TATCACG556.184564E-1140.9090921
CGTTTTT64000.040.2890621
Produced by FastQC (version 0.11.5)