FastQCFastQC Report
Thu 26 May 2016
SRR1545439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545439_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1791638
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154030.859716081038692No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31380.175146988398326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGC207.034344E-445.022
TCGTTGA3850.042.07792324
TAGCCGT700.041.78571344
CGTTTTT114300.041.732281
CGTTGAT4050.040.025
TACGGGT1250.039.6000024
TCGATTG356.250246E-638.5714261
TCTATCG356.250246E-638.57142614
AGGGCGA19900.037.9899526
AATACGG1600.037.968752
GGGCGAT37550.037.6897477
ATCGTTG4250.037.58823823
CCGACGG301.1401765E-437.5000042
CGTAATC301.1401765E-437.50000433
ACGTTAG1150.037.1739161
AATCGTT4300.037.1511622
GCGATCG850.037.0588239
CGGGTAT1650.036.8181846
TTACACG6100.036.51639634
TGGGCGA11700.036.3461536
Produced by FastQC (version 0.11.5)