##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545439_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1791638
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.909414736682297	31.0	31.0	33.0	28.0	34.0
2	31.203714701295684	31.0	31.0	34.0	28.0	34.0
3	31.272011980098657	31.0	31.0	34.0	28.0	34.0
4	35.11685507898359	35.0	35.0	37.0	32.0	37.0
5	34.988737680268	35.0	35.0	37.0	32.0	37.0
6	35.04029273770706	37.0	35.0	37.0	32.0	37.0
7	35.54741192138144	37.0	35.0	37.0	35.0	37.0
8	35.467843392471025	37.0	35.0	37.0	35.0	37.0
9	37.135229884608385	39.0	37.0	39.0	34.0	39.0
10	36.53445227216659	39.0	35.0	39.0	32.0	39.0
11	36.25254097088809	38.0	35.0	39.0	31.0	39.0
12	35.719644258494185	37.0	35.0	39.0	30.0	39.0
13	35.35043518835836	37.0	35.0	39.0	30.0	39.0
14	36.38878501125785	38.0	35.0	40.0	30.0	41.0
15	36.64219334486096	38.0	35.0	40.0	31.0	41.0
16	36.68675703462418	38.0	35.0	40.0	31.0	41.0
17	36.58207405737097	38.0	35.0	40.0	31.0	41.0
18	36.479194457809	38.0	35.0	40.0	31.0	41.0
19	36.3864915792141	38.0	35.0	40.0	30.0	41.0
20	36.24582700299949	38.0	35.0	40.0	30.0	41.0
21	36.07870395693773	38.0	34.0	40.0	30.0	41.0
22	35.98283246950556	37.0	34.0	40.0	30.0	41.0
23	35.899099594895844	37.0	34.0	40.0	30.0	41.0
24	35.770204137219686	37.0	34.0	40.0	30.0	41.0
25	35.61759630014545	37.0	34.0	40.0	29.0	41.0
26	35.4608324895989	37.0	34.0	40.0	29.0	41.0
27	35.371892647956784	37.0	34.0	40.0	29.0	41.0
28	35.36088540207341	37.0	34.0	40.0	28.0	41.0
29	35.36032223027196	37.0	34.0	40.0	29.0	41.0
30	35.236839696411884	37.0	34.0	40.0	27.0	41.0
31	35.08693720494877	37.0	34.0	40.0	27.0	41.0
32	34.86216746909811	37.0	34.0	40.0	25.0	41.0
33	34.57845892976148	37.0	33.0	40.0	24.0	41.0
34	34.35698561874664	37.0	33.0	40.0	23.0	41.0
35	34.13491732146784	36.0	33.0	40.0	23.0	41.0
36	33.961494453678704	36.0	33.0	40.0	22.0	41.0
37	33.90943538817551	36.0	33.0	40.0	22.0	41.0
38	33.85787809814259	36.0	33.0	40.0	22.0	41.0
39	33.78188618459756	36.0	33.0	40.0	22.0	41.0
40	33.592211149797	36.0	32.0	40.0	21.0	41.0
41	33.50759137727599	36.0	32.0	40.0	20.0	41.0
42	33.45156164359095	36.0	32.0	40.0	21.0	41.0
43	33.35338835188805	35.0	32.0	40.0	20.0	41.0
44	33.273235999682974	35.0	32.0	40.0	20.0	41.0
45	33.203161576166615	35.0	32.0	40.0	20.0	41.0
46	33.13969730492432	35.0	32.0	40.0	20.0	41.0
47	33.05935406594413	35.0	32.0	39.0	20.0	41.0
48	32.99422372153303	35.0	31.0	39.0	20.0	41.0
49	32.97527681373135	35.0	31.0	39.0	20.0	41.0
50	32.89079936906897	35.0	31.0	39.0	19.0	41.0
51	32.713571603192165	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	7.0
13	17.0
14	41.0
15	102.0
16	303.0
17	745.0
18	1655.0
19	3233.0
20	5414.0
21	8223.0
22	11812.0
23	16547.0
24	22421.0
25	30840.0
26	39123.0
27	44766.0
28	47808.0
29	52317.0
30	59730.0
31	70420.0
32	84343.0
33	105697.0
34	158188.0
35	199173.0
36	141815.0
37	170983.0
38	226549.0
39	289269.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.500852292706455	21.759920251747282	26.590360329486202	18.148867126060065
2	35.07589144682129	24.970613483304106	26.728278815251745	13.225216254622865
3	29.103479609162118	25.03803781790741	32.59877274315458	13.25970982977588
4	26.19161906590505	27.856408493233566	31.306045082767834	14.645927358093545
5	22.61930144370682	31.169131264239763	32.00573999881672	14.205827293236691
6	21.041359917572635	39.86050753556243	29.43825705862457	9.65987548824037
7	82.28860964101007	5.129384395731727	10.536224393543785	2.0457815697144177
8	82.87968886571953	4.521616531911022	10.425376108343315	2.173318494026137
9	77.79785871922788	6.284249385199465	12.284457016428542	3.633434879144113
10	43.250198979927866	24.86255594042993	18.763611845696506	13.123633233945696
11	36.75541599363264	24.496522176912972	24.577342074682498	14.170719754771891
12	33.779145117484674	22.391353610494978	28.848070871459523	14.981430400560827
13	22.234737151143253	32.156719158669326	28.987607987774318	16.6209357024131
14	17.961887390198246	34.48760296443813	30.855284382224536	16.695225263139093
15	17.1416882204999	23.961927576887742	42.97681786164392	15.919566340968434
16	19.220567994204185	20.12220102498384	41.87888401563262	18.77834696517935
17	19.43757611749695	20.72583859016163	31.980344243647433	27.856241048693985
18	22.35317625547125	23.56502820324195	34.39104327994829	19.690752261338506
19	28.42002681345227	24.083659757160767	28.4752276966664	19.02108573272056
20	30.09659317339775	22.3821441608182	28.5359542496866	18.98530841609745
21	24.613063576459083	25.761788932808972	30.38543500416937	19.239712486562578
22	23.577307469477653	24.61585431878538	28.54923818315977	23.2576000285772
23	21.818414210906447	28.10500781966	28.85521517181484	21.221362797618713
24	20.95830742594207	23.546776748427973	36.82719388626497	18.667721939364984
25	20.330781106451195	25.270506653687853	33.932970834510094	20.465741405350858
26	19.78887476153107	30.247293259017727	29.633441576925694	20.33039040252551
27	20.681186712940896	29.784699811010928	30.393193267836473	19.140920208211703
28	18.99892723864977	27.079075125667128	35.256508290179156	18.665489345503946
29	19.315564862991298	25.827371377476922	34.55346448333871	20.303599276193072
30	20.32681825234785	28.29321548214539	31.821216116202045	19.558750149304714
31	25.071080207050755	26.903314173957014	28.602541361592017	19.42306425740021
32	27.092917207605556	26.03098393760347	29.114865837853404	17.761233016937574
33	25.897195750480844	28.060132683053162	27.13148526655496	18.91118629991103
34	21.086235054179472	27.90653022541384	29.689758757070344	21.317475963336342
35	22.177526933454192	26.87713701093636	30.02755020824519	20.917785847364257
36	26.009216147458357	28.978845056869744	26.13574840453261	18.876190391139282
37	21.506744107905725	31.761773304651946	28.39691946699054	18.33456312045179
38	21.79971623732026	30.89815018435644	26.77739588019455	20.52473769812875
39	22.089953439255027	30.552209765588806	26.767851541438613	20.589985253717547
40	23.967899765466015	26.954328943681706	27.986624530178528	21.09114676067375
41	19.372551821294255	27.190090855407174	28.767306788536523	24.670050534762044
42	22.559356298537985	27.91540478601146	26.208586779248932	23.316652136201622
43	22.409158546536744	27.824594030713794	27.379024110897404	22.387223311852058
44	21.426370728908406	29.499206871030864	28.19146501692864	20.882957383132084
45	19.73266921107947	32.76102650200542	25.93950340414749	21.566800882767613
46	20.57831995079363	31.66097169182614	27.220677391303376	20.540030966076852
47	22.242551229656886	28.767697492462208	27.66831246044123	21.321438817439684
48	22.453810423757478	25.96847130949444	30.295963805188325	21.281754461559757
49	21.638299701167313	27.228603099510057	30.5608052519538	20.572291947368832
50	19.749525294730297	32.269074444726	27.585315783657187	20.396084476886514
51	20.108358943045417	31.42833541150612	26.405724817178474	22.057580828269995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1625.0
1	2810.5
2	3996.0
3	16106.0
4	28216.0
5	20620.5
6	13025.0
7	13047.5
8	13070.0
9	13552.5
10	14035.0
11	13696.0
12	13357.0
13	13260.0
14	13163.0
15	12844.0
16	12525.0
17	11939.5
18	11354.0
19	11095.0
20	10836.0
21	11310.0
22	11784.0
23	11680.5
24	11577.0
25	13052.0
26	15868.5
27	17210.0
28	22366.0
29	27522.0
30	30654.0
31	33786.0
32	39371.0
33	44956.0
34	49252.0
35	53548.0
36	55214.0
37	56880.0
38	63730.5
39	70581.0
40	85011.0
41	99441.0
42	114620.0
43	129799.0
44	131960.0
45	134121.0
46	132986.0
47	131851.0
48	125525.0
49	119199.0
50	116357.0
51	113515.0
52	108002.5
53	102490.0
54	98668.0
55	94846.0
56	91571.0
57	88296.0
58	85669.5
59	83043.0
60	77884.0
61	72725.0
62	62879.0
63	53033.0
64	43424.0
65	33815.0
66	28065.0
67	22315.0
68	17930.5
69	13546.0
70	11360.5
71	9175.0
72	7566.5
73	5958.0
74	4563.0
75	2574.0
76	1980.0
77	1433.5
78	887.0
79	674.0
80	461.0
81	347.5
82	234.0
83	155.0
84	76.0
85	55.0
86	34.0
87	35.0
88	36.0
89	25.0
90	14.0
91	9.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1791638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.991683647675583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33166802473109	16.653052784175753
2	5.454574622086946	2.2900140979897756
3	2.3043776718972713	1.4511830127970404
4	1.3888737374270166	1.166191924905311
5	0.9735488696493504	1.0218214943615656
6	0.7552268251358599	0.9512089557294228
7	0.5714472086713439	0.8396947318043276
8	0.5075666567701446	0.8523742951221767
9	0.43477856885072214	0.8214060756693146
>10	5.700105740961603	28.58858864872225
>50	2.112634049862377	31.318292942948105
>100	0.46199712012969923	12.5145211778038
>500	0.001600451913342146	0.22576633133507326
>1k	0.0013337099277851217	0.4434135857744159
>5k	0.0	0.0
>10k+	2.667419855570243E-4	0.8624699408616402
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15403	0.859716081038692	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3138	0.175146988398326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.581484652591651E-5	0.0	0.0	0.007255930048369146	0.0
2	5.581484652591651E-5	0.0	0.0	0.028019052956010086	0.0
3	5.581484652591651E-5	0.0	0.0	0.04314487636453346	0.0
4	5.581484652591651E-5	0.0	0.0	0.061898664797241404	0.0
5	5.581484652591651E-5	0.0	0.0	0.10264350276116045	0.0
6	5.581484652591651E-5	0.0	0.0	0.16683057626596445	0.0
7	5.581484652591651E-5	0.0	0.0	0.20606841337368376	0.0
8	5.581484652591651E-5	0.0	0.0	0.2958745014338834	0.0
9	5.581484652591651E-5	0.0	0.0	0.34370782490659385	0.0
10	5.581484652591651E-5	0.0	0.0	0.416490384776389	0.0
11	5.581484652591651E-5	0.0	0.0	0.4915055385072208	0.0
12	5.581484652591651E-5	0.0	0.0	0.5563623901703357	0.0
13	1.1162969305183302E-4	0.0	0.0	0.5863349627547529	0.0
14	1.1162969305183302E-4	0.0	0.0	0.600958452544543	0.0
15	1.1162969305183302E-4	0.0	0.0	0.619656426130725	0.0
16	1.1162969305183302E-4	0.0	0.0	0.6542058161302674	0.0
17	1.1162969305183302E-4	0.0	0.0	0.6908761702977946	0.0
18	1.1162969305183302E-4	0.0	0.0	0.7436770151113116	0.0
19	1.1162969305183302E-4	0.0	0.0	0.7696867335923886	0.0
20	1.6744453957774952E-4	0.0	0.0	0.7972592677761914	0.0
21	1.6744453957774952E-4	0.0	0.0	0.830915620231319	0.0
22	1.6744453957774952E-4	0.0	0.0	0.8692046049480978	0.0
23	1.6744453957774952E-4	0.0	0.0	0.9139681118618828	0.0
24	1.6744453957774952E-4	0.0	0.0	0.9467314267725958	0.0
25	1.6744453957774952E-4	0.0	0.0	0.9766481845104871	0.0
26	1.6744453957774952E-4	0.0	0.0	1.0044439780803935	0.0
27	1.6744453957774952E-4	0.0	0.0	1.0364816999862696	0.0
28	1.6744453957774952E-4	0.0	0.0	1.0705287563670787	0.0
29	1.6744453957774952E-4	0.0	0.0	1.103682775203473	0.0
30	1.6744453957774952E-4	0.0	0.0	1.1507905056713466	0.0
31	1.6744453957774952E-4	0.0	0.0	1.1906981209373768	0.0
32	2.2325938610366603E-4	0.0	0.0	1.234066256688014	0.0
33	2.2325938610366603E-4	0.0	0.0	1.2766529845872883	0.0
34	2.2325938610366603E-4	0.0	0.0	1.3195187867191922	0.0
35	2.2325938610366603E-4	0.0	0.0	1.3736591878493312	0.0
36	2.2325938610366603E-4	0.0	0.0	1.4208785480102566	0.0
37	2.2325938610366603E-4	0.0	0.0	1.468711871482967	0.0
38	2.2325938610366603E-4	0.0	0.0	1.5177173067327216	0.0
39	2.2325938610366603E-4	0.0	0.0	1.5821276396236292	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTCGC	20	7.034344E-4	45.0	22
TCGTTGA	385	0.0	42.077923	24
TAGCCGT	70	0.0	41.785713	44
CGTTTTT	11430	0.0	41.73228	1
CGTTGAT	405	0.0	40.0	25
TACGGGT	125	0.0	39.600002	4
TCGATTG	35	6.250246E-6	38.571426	1
TCTATCG	35	6.250246E-6	38.571426	14
AGGGCGA	1990	0.0	37.989952	6
AATACGG	160	0.0	37.96875	2
GGGCGAT	3755	0.0	37.689747	7
ATCGTTG	425	0.0	37.588238	23
CCGACGG	30	1.1401765E-4	37.500004	2
CGTAATC	30	1.1401765E-4	37.500004	33
ACGTTAG	115	0.0	37.173916	1
AATCGTT	430	0.0	37.15116	22
GCGATCG	85	0.0	37.058823	9
CGGGTAT	165	0.0	36.818184	6
TTACACG	610	0.0	36.516396	34
TGGGCGA	1170	0.0	36.346153	6
>>END_MODULE