FastQCFastQC Report
Thu 26 May 2016
SRR1545438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545438_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences552643
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61251.1083104282511496No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13740.24862343321095173No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10110.18293907640194484No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7270.13154966225936093No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5630.10187408507843218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.10060744458900231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG551.8189894E-1245.0000042
TCACGAC406.8048394E-945.025
AAACTCG207.030106E-445.023
CAACGCG253.8881673E-545.01
TAGCGGA207.030106E-445.03
GTTAGCG253.8881673E-545.01
ACGGGCC406.8048394E-945.05
TATCTCG253.8881673E-545.01
TATCTAG207.030106E-445.01
GTGTACG207.030106E-445.01
CGGTCTA406.8048394E-945.031
AATACTC207.030106E-445.010
GCCGTAT253.8881673E-545.029
CGCCCCA253.8881673E-545.037
ACACGCG302.1634478E-644.99999636
CTCATAC302.1634478E-644.99999630
CATACGC302.1634478E-644.99999632
TTACACG302.1634478E-644.99999634
GCGCGAC750.042.09
CGACCCG750.042.032