##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545438_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	552643
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98758873269	31.0	31.0	33.0	30.0	34.0
2	31.277028750929624	31.0	31.0	34.0	30.0	34.0
3	31.320897939537822	31.0	31.0	34.0	28.0	34.0
4	35.1724422457174	37.0	35.0	37.0	32.0	37.0
5	35.05623521875786	37.0	35.0	37.0	32.0	37.0
6	35.11193844851016	37.0	35.0	37.0	32.0	37.0
7	35.60759658586103	37.0	35.0	37.0	35.0	37.0
8	35.534929420982444	37.0	35.0	37.0	35.0	37.0
9	37.213278735096615	39.0	37.0	39.0	34.0	39.0
10	36.65089578624899	39.0	35.0	39.0	32.0	39.0
11	36.357071382429524	38.0	35.0	39.0	32.0	39.0
12	35.61459748879476	37.0	35.0	39.0	31.0	39.0
13	35.19062034622713	37.0	35.0	39.0	30.0	39.0
14	36.211248491340704	38.0	35.0	40.0	30.0	41.0
15	36.53389258526752	38.0	35.0	40.0	31.0	41.0
16	36.588600597492416	38.0	35.0	40.0	31.0	41.0
17	36.51338386625724	38.0	35.0	40.0	31.0	41.0
18	36.397761303409254	38.0	35.0	40.0	31.0	41.0
19	36.23307813543282	37.0	35.0	40.0	31.0	41.0
20	36.01652422992782	37.0	34.0	40.0	30.0	41.0
21	35.87492648961445	36.0	34.0	40.0	30.0	41.0
22	35.7558025705564	36.0	34.0	40.0	30.0	41.0
23	35.71077169167075	36.0	34.0	40.0	30.0	41.0
24	35.57480507307611	36.0	34.0	40.0	30.0	41.0
25	35.44127583268041	36.0	34.0	40.0	29.0	41.0
26	35.27340977810268	36.0	34.0	40.0	29.0	41.0
27	35.16454021854977	36.0	34.0	40.0	29.0	41.0
28	35.186545382823994	36.0	34.0	40.0	29.0	41.0
29	35.229936867019035	36.0	34.0	40.0	29.0	41.0
30	35.08898692284169	36.0	34.0	40.0	28.0	41.0
31	34.86493450563926	36.0	34.0	40.0	27.0	41.0
32	34.560660317782	35.0	33.0	40.0	25.0	41.0
33	34.19508434920916	35.0	33.0	40.0	23.0	41.0
34	33.94198062763846	35.0	33.0	40.0	23.0	41.0
35	33.698584800676024	35.0	33.0	40.0	22.0	41.0
36	33.48267145336139	35.0	33.0	40.0	21.0	41.0
37	33.41135959380649	35.0	32.0	40.0	20.0	41.0
38	33.39416585390568	35.0	32.0	40.0	20.0	41.0
39	33.33413433265236	35.0	32.0	40.0	20.0	41.0
40	33.155697258447134	35.0	32.0	40.0	18.0	41.0
41	33.122113190613106	35.0	32.0	40.0	18.0	41.0
42	33.040051172275774	35.0	32.0	40.0	18.0	41.0
43	32.92238750875339	35.0	32.0	40.0	18.0	41.0
44	32.84145460993806	35.0	32.0	39.0	18.0	41.0
45	32.79825493130285	35.0	31.0	39.0	18.0	41.0
46	32.73679934424212	35.0	31.0	39.0	18.0	41.0
47	32.661915558507026	35.0	31.0	39.0	18.0	41.0
48	32.61051347795955	35.0	31.0	39.0	18.0	41.0
49	32.63269959087512	35.0	31.0	39.0	18.0	41.0
50	32.52617150674124	35.0	31.0	39.0	18.0	41.0
51	32.338102536357106	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	9.0
15	28.0
16	65.0
17	233.0
18	488.0
19	937.0
20	1685.0
21	2554.0
22	3542.0
23	5272.0
24	7144.0
25	10384.0
26	13489.0
27	15353.0
28	15653.0
29	16340.0
30	18811.0
31	21782.0
32	26757.0
33	33829.0
34	53494.0
35	71398.0
36	40271.0
37	46940.0
38	62389.0
39	83757.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.76101027245437	22.589809334416614	26.043937949091912	19.605242444037106
2	34.85686057726235	24.977064759709254	27.94480342644347	12.22127123658492
3	28.189807886827484	24.43059986284093	34.9453444628811	12.434247787450488
4	26.428815709237245	26.546794223395576	32.75333262160201	14.271057445765168
5	22.10142895142072	30.714765228185286	33.36222479973509	13.821581020658908
6	20.442129910267568	39.58595331886951	30.40154313001341	9.570373640849517
7	80.82613911693444	5.322242387943031	12.081397936823592	1.770220558298938
8	81.74246303671629	4.588314698639085	12.007389942512617	1.661832322132009
9	76.66486321187458	6.192243455540014	13.959463885365416	3.183429447219996
10	46.607665346344746	22.491192324882427	19.353723832564604	11.547418496208222
11	41.318717508409584	21.339092325425273	24.667823531647013	12.674366634518124
12	36.82558179511909	21.6997229676301	28.772100614682532	12.702594622568276
13	20.449367855921455	37.19851694493552	28.304710274082908	14.04740492506012
14	15.400719813695279	38.256161753609476	31.894188472485858	14.448929960209394
15	15.554526158840337	22.79717647740042	47.800478790105004	13.847818573654239
16	16.262759141072987	19.162099221377996	46.31887131475473	18.256270322794283
17	16.5265822601571	19.694269175579894	33.0274698132429	30.7516787510201
18	20.82881715682638	23.004724569025573	37.03385368130964	19.132604592838415
19	28.41418420209792	22.256682885696552	30.242670222910633	19.086462689294898
20	29.294137444968992	22.13273306637377	30.598415251799082	17.97471423685815
21	22.544391225438485	26.16535448743583	32.39957802776838	18.890676259357306
22	21.693570713824293	24.658957048221	29.359098007212616	24.28837423074209
23	19.49432092689132	28.27195856999908	29.58655044938595	22.64717005372365
24	18.509960317962953	23.43827751369329	41.627958736471825	16.42380343187193
25	17.47041037342371	24.743098166447417	38.79936957493354	18.987121885195325
26	16.78045320396712	31.870303251828037	31.927121125211034	19.42212241899382
27	17.93309604934831	32.61979252428783	32.47430981664474	16.972801609719113
28	16.19399865736108	27.81234902097738	39.63408565746784	16.359566664193704
29	16.120171611691454	24.623672063158313	38.674153115121335	20.582003210028898
30	17.619331105252396	30.35558217511124	34.06484837408598	17.960238345550383
31	26.25999062686038	27.093440068905245	29.00154349191069	17.645025812323688
32	27.54183080216342	26.036700003438025	30.250451014488554	16.171018179909996
33	25.89465531998053	27.87513819952483	27.799320718800384	18.430885761694256
34	19.504634999448108	27.789187594884947	30.6664519409456	22.03972546472135
35	20.85957842585539	25.813047482732976	31.944854092063053	21.382519999348585
36	27.786654313906084	27.352739471955672	27.246884516767604	17.613721697370636
37	19.924073950090744	33.39262417148141	30.138443805494685	16.54485807293316
38	20.429644454014618	33.36222479973509	26.229229357831368	19.978901388418922
39	20.93069124190481	31.895997958899326	28.50592516326091	18.667385635934952
40	23.15853091417063	27.671209080726616	27.20834245616067	21.961917548942083
41	17.839907499054544	26.747285318008192	29.1897300789117	26.223077104025567
42	20.669763301082252	27.280902861340866	26.379959576073524	25.669374261503357
43	21.365510827061954	26.911767632992728	28.119418865343448	23.603302674601867
44	18.610024916627914	30.528569076239094	30.132110603047536	20.729295404085455
45	17.887858889011532	35.997379863673295	25.78753372430303	20.32722752301214
46	20.751733035612503	33.29364526466453	26.729552351156173	19.225069348566795
47	20.821217313889797	29.03320950414644	28.572876160559346	21.572697021404416
48	22.04967763999544	26.265419086100795	31.9227783578187	19.762124916085067
49	20.58055562089812	26.620259371782506	32.83385476700148	19.96533024031789
50	19.699335737537616	33.27319806819231	28.189445989544787	18.838020204725293
51	18.62468175657703	32.64204920717353	27.152429325984407	21.580839710265035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	810.0
1	1122.0
2	1434.0
3	6060.0
4	10686.0
5	7841.5
6	4997.0
7	5143.0
8	5289.0
9	5558.5
10	5828.0
11	5887.0
12	5946.0
13	5769.0
14	5592.0
15	5169.0
16	4746.0
17	4529.0
18	4312.0
19	3977.5
20	3643.0
21	3563.5
22	3484.0
23	3470.5
24	3457.0
25	3740.5
26	5000.0
27	5976.0
28	7095.5
29	8215.0
30	9217.0
31	10219.0
32	11194.5
33	12170.0
34	13345.5
35	14521.0
36	15904.0
37	17287.0
38	19393.5
39	21500.0
40	27666.0
41	33832.0
42	41528.0
43	49224.0
44	50284.0
45	51344.0
46	48272.0
47	45200.0
48	42475.5
49	39751.0
50	36220.0
51	32689.0
52	31061.5
53	29434.0
54	27476.0
55	25518.0
56	24836.0
57	24154.0
58	21917.5
59	19681.0
60	18102.0
61	16523.0
62	14197.0
63	11871.0
64	9517.0
65	7163.0
66	5987.5
67	4812.0
68	3828.5
69	2845.0
70	2377.0
71	1909.0
72	1534.5
73	1160.0
74	922.5
75	521.0
76	357.0
77	273.0
78	189.0
79	133.0
80	77.0
81	65.5
82	54.0
83	37.0
84	20.0
85	13.0
86	6.0
87	5.0
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	552643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.096444995711543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2792990386746	19.143392326355293
2	5.440401518225717	2.7306947491347455
3	2.5104359995543404	1.8900905693420886
4	1.640717158460512	1.6470467168329748
5	1.248407576946742	1.5665296043536714
6	0.994051987968415	1.496830262335622
7	0.857938444958248	1.5071843495520674
8	0.7141374006603098	1.4337847996041504
9	0.6455719926801279	1.458140580456185
>10	8.983901320993434	53.823969476187195
>50	0.6411907772670973	9.829080270331303
>100	0.03890371008137444	1.504836655261252
>500	0.002881756302324033	0.4297725254783126
>1k	0.0014408781511620165	0.43121896224895895
>5k	7.204390755810083E-4	1.1074281525261525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6125	1.1083104282511496	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1374	0.24862343321095173	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1011	0.18293907640194484	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	727	0.13154966225936093	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	563	0.10187408507843218	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	556	0.10060744458900231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011399764404868966	0.0
2	0.0	0.0	0.0	0.045418108978128736	0.0
3	0.0	0.0	0.0	0.06134158941667586	0.0
4	0.0	0.0	0.0	0.08034119675812414	0.0
5	0.0	0.0	0.0	0.1237688706814345	0.0
6	0.0	0.0	0.0	0.1738916443345885	0.0
7	0.0	0.0	0.0	0.20012919732992185	0.0
8	0.0	0.0	0.0	0.2612898381052506	0.0
9	0.0	0.0	0.0	0.29042256936213795	0.0
10	0.0	0.0	0.0	0.3380120620364322	0.0
11	0.0	0.0	0.0	0.40604875118295175	0.0
12	0.0	0.0	0.0	0.4590667030976598	0.0
13	0.0	0.0	0.0	0.48892322891993567	0.0
14	0.0	0.0	0.0	0.502313428379623	0.0
15	0.0	0.0	0.0	0.5213130357210712	0.0
16	0.0	0.0	0.0	0.556598020783761	0.0
17	0.0	0.0	0.0	0.6012923351965012	0.0
18	0.0	0.0	0.0	0.6611863354824	0.0
19	0.0	0.0	0.0	0.6876048371190805	0.0
20	0.0	0.0	0.0	0.7163756710932736	0.0
21	0.0	0.0	0.0	0.7569081667550299	0.0
22	0.0	0.0	0.0	0.7934597923071495	0.0
23	0.0	0.0	0.0	0.8405064390574023	0.0
24	0.0	0.0	0.0	0.8756104754787448	0.0
25	0.0	0.0	0.0	0.9049241553769793	0.0
26	0.0	0.0	0.0	0.9378568081021563	0.0
27	0.0	0.0	0.0	0.9707894608273334	0.0
28	0.0	0.0	0.0	1.0077029836621472	0.0
29	0.0	0.0	0.0	1.043349866007531	0.0
30	0.0	0.0	0.0	1.0936535883020322	0.0
31	0.0	0.0	0.0	1.141966875541715	0.0
32	0.0	0.0	0.0	1.1826803198448184	0.0
33	0.0	0.0	0.0	1.2244794559960046	0.0
34	0.0	0.0	0.0	1.269173770408745	0.0
35	0.0	0.0	0.0	1.3268963869984782	0.0
36	0.0	0.0	0.0	1.3829904658160874	0.0
37	0.0	0.0	0.0	1.4419797228952507	0.0
38	0.0	0.0	0.0	1.4915596506243634	0.0
39	0.0	0.0	0.0	1.5478346780833196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	55	1.8189894E-12	45.000004	2
TCACGAC	40	6.8048394E-9	45.0	25
AAACTCG	20	7.030106E-4	45.0	23
CAACGCG	25	3.8881673E-5	45.0	1
TAGCGGA	20	7.030106E-4	45.0	3
GTTAGCG	25	3.8881673E-5	45.0	1
ACGGGCC	40	6.8048394E-9	45.0	5
TATCTCG	25	3.8881673E-5	45.0	1
TATCTAG	20	7.030106E-4	45.0	1
GTGTACG	20	7.030106E-4	45.0	1
CGGTCTA	40	6.8048394E-9	45.0	31
AATACTC	20	7.030106E-4	45.0	10
GCCGTAT	25	3.8881673E-5	45.0	29
CGCCCCA	25	3.8881673E-5	45.0	37
ACACGCG	30	2.1634478E-6	44.999996	36
CTCATAC	30	2.1634478E-6	44.999996	30
CATACGC	30	2.1634478E-6	44.999996	32
TTACACG	30	2.1634478E-6	44.999996	34
GCGCGAC	75	0.0	42.0	9
CGACCCG	75	0.0	42.0	32
>>END_MODULE