FastQCFastQC Report
Thu 26 May 2016
SRR1545437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545437_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1052555
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66050.6275206521274423No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22450.2132905168851034No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16650.158186508068462No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11910.11315323189762054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC207.033014E-445.00000418
ACGCCTA207.033014E-445.00000438
ACACCGT207.033014E-445.00000419
TTCGTAC207.033014E-445.00000433
CGTTATG207.033014E-445.0000042
GCGATAT850.045.0000049
CGTACAT207.033014E-445.00000435
CTCGACC207.033014E-445.00000428
ATCGGAT207.033014E-445.00000440
TGCGTTA207.033014E-445.00000422
GTCGATG502.1827873E-1145.01
CTAACCG253.890582E-545.03
CGGATAT253.890582E-545.034
TACGAAG351.2117926E-745.01
ACGGATA253.890582E-545.033
CGGGCTA453.8562575E-1045.06
ACGTAAG253.890582E-545.01
CGCGTAA253.890582E-545.031
CGTAAGG1250.041.3999982
ACGTAGG603.6379788E-1241.2499962