##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545437_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1052555
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00482730118616	31.0	31.0	33.0	30.0	34.0
2	31.290966267795984	31.0	31.0	34.0	30.0	34.0
3	31.383096370260937	31.0	31.0	34.0	30.0	34.0
4	35.224855708252775	37.0	35.0	37.0	33.0	37.0
5	35.10077668150358	37.0	35.0	37.0	32.0	37.0
6	35.15874799891692	37.0	35.0	37.0	32.0	37.0
7	35.61854154889768	37.0	35.0	37.0	35.0	37.0
8	35.531405009714454	37.0	35.0	37.0	35.0	37.0
9	37.234108431388385	39.0	37.0	39.0	34.0	39.0
10	36.66378954068909	39.0	35.0	39.0	32.0	39.0
11	36.37388164989003	38.0	35.0	39.0	32.0	39.0
12	35.62228197101339	37.0	35.0	39.0	31.0	39.0
13	35.1263829443592	37.0	35.0	39.0	30.0	39.0
14	36.155037979012974	38.0	35.0	40.0	30.0	41.0
15	36.51676824488982	38.0	35.0	40.0	31.0	41.0
16	36.60446437478326	38.0	35.0	40.0	31.0	41.0
17	36.52830208397661	38.0	35.0	40.0	31.0	41.0
18	36.4659528480697	38.0	35.0	40.0	31.0	41.0
19	36.38470103700044	37.0	35.0	40.0	31.0	41.0
20	36.21933770681817	37.0	35.0	40.0	31.0	41.0
21	36.04251559300939	37.0	34.0	40.0	30.0	41.0
22	35.94447036021871	37.0	34.0	40.0	30.0	41.0
23	35.899908318330155	37.0	34.0	40.0	30.0	41.0
24	35.77547776600748	37.0	34.0	40.0	30.0	41.0
25	35.61935860833876	36.0	34.0	40.0	30.0	41.0
26	35.49731177943195	36.0	34.0	40.0	29.0	41.0
27	35.39586054885493	36.0	34.0	40.0	29.0	41.0
28	35.43744412406002	36.0	34.0	40.0	29.0	41.0
29	35.481170105125145	36.0	34.0	40.0	29.0	41.0
30	35.38457657794604	36.0	34.0	40.0	29.0	41.0
31	35.20350955532015	36.0	34.0	40.0	29.0	41.0
32	34.98312962268005	36.0	34.0	40.0	27.0	41.0
33	34.72461866600795	36.0	33.0	40.0	26.0	41.0
34	34.54905064343431	36.0	34.0	40.0	25.0	41.0
35	34.35806964956701	36.0	33.0	40.0	24.0	41.0
36	34.15803259687142	36.0	33.0	40.0	23.0	41.0
37	34.099266071606706	36.0	33.0	40.0	23.0	41.0
38	34.07087895644408	36.0	33.0	40.0	23.0	41.0
39	34.01190911638822	36.0	33.0	40.0	23.0	41.0
40	33.83658526157778	35.0	33.0	40.0	23.0	41.0
41	33.786955551016334	35.0	33.0	40.0	23.0	41.0
42	33.76938307261853	35.0	33.0	40.0	23.0	41.0
43	33.67594187477139	35.0	33.0	40.0	23.0	41.0
44	33.632619673081216	35.0	33.0	40.0	23.0	41.0
45	33.56911990347298	35.0	33.0	40.0	23.0	41.0
46	33.494143298924996	35.0	32.0	39.0	23.0	41.0
47	33.432446760501826	35.0	32.0	39.0	23.0	41.0
48	33.38221280598164	35.0	32.0	39.0	22.0	41.0
49	33.39657405076219	35.0	32.0	39.0	22.0	41.0
50	33.293517203376545	35.0	32.0	39.0	22.0	41.0
51	33.10580064699707	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	15.0
15	33.0
16	128.0
17	321.0
18	766.0
19	1421.0
20	2464.0
21	3822.0
22	5749.0
23	8247.0
24	11424.0
25	16160.0
26	20714.0
27	23969.0
28	26373.0
29	28800.0
30	33680.0
31	41290.0
32	50514.0
33	64563.0
34	104043.0
35	136515.0
36	78726.0
37	93669.0
38	126934.0
39	172154.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.070575884395595	23.56057403176081	27.00371952059512	16.365130563248474
2	32.852154994275836	25.078404453924026	29.111447857831656	12.957992693968487
3	29.175007481794303	24.600139660160277	32.455311123884265	13.769541734161162
4	26.051750264831767	27.3941029209875	31.684615055745308	14.869531758435425
5	22.782467424505132	31.0298274199448	31.741334182061742	14.446370973488321
6	21.379595365562846	40.35418576701455	28.485827343939274	9.780391523483335
7	84.27455097358333	4.780557785578901	9.130069212535211	1.814822028302559
8	85.04458199334002	4.117314534632395	8.94936606638133	1.8887374056462607
9	80.1611317223328	6.224472830398412	10.480212435454678	3.1341830118141094
10	48.176579846183806	23.427089320748085	16.828289258043522	11.568041575024584
11	43.119646954315925	22.490226163953427	21.356318672183402	13.033808209547246
12	39.2177130886272	21.965788011077805	25.581466051655262	13.235032848639738
13	21.948401746227038	36.94001738626485	25.793236457952318	15.318344409555795
14	15.436057973217551	39.499883616533104	29.532328476896698	15.53172993335265
15	15.11778481884557	23.917229978480933	46.63480768225888	14.330177520414612
16	16.923676197443363	19.626242809164367	45.225380146405655	18.224700846986618
17	16.91911586567923	19.590330196521798	31.47046947665443	32.02008446114455
18	21.01125356869712	23.271658013120454	34.99646099253721	20.720627425645215
19	30.16716466122911	23.558008845143487	27.375006531725184	18.899819961902228
20	31.36890708799065	22.46476431160367	26.992128677361276	19.1741999230444
21	23.280968690472232	26.627777170789173	29.478364551021087	20.612889587717508
22	22.51549800247968	25.989900765280677	26.623026825201535	24.871574407038114
23	21.03595536575286	29.432001178085702	27.506306083767594	22.02573737239384
24	20.034107481319268	23.436209984276356	38.55237968562213	17.977302848782248
25	18.302891535359198	25.577570768273393	36.270978713701425	19.84855898266599
26	18.272584330510043	32.952102265439805	29.001334847110126	19.773978556940015
27	19.043280398649003	33.369562635681746	30.011448332866212	17.575708632803035
28	16.836459852454265	28.93463999505964	37.515759271487	16.71314088099909
29	17.507968704723268	25.687303751347912	36.56968044425232	20.2350470996765
30	19.35015272361064	29.60272859850554	31.963175321004606	19.083943356879214
31	27.499560593033145	26.431397884196073	27.040107167796457	19.028934354974325
32	29.577266746155782	26.676515716518377	27.618224225812426	16.127993311513414
33	27.283039841148444	27.209409484539997	26.402895810670223	19.10465486364133
34	20.51940278655272	27.720546669770226	29.006275206521277	22.75377533715578
35	22.019181895482898	25.052182546280243	30.980328818921578	21.948306739315285
36	28.769042947874457	27.183377590719726	25.740792642664758	18.306786818741063
37	21.384915752621005	33.669404449173676	28.13696196398288	16.80871783422244
38	21.818432290949165	33.40281505479524	24.192465001828882	20.586287652426712
39	22.125494629734312	31.480445202388474	26.400615644788157	19.993444523089053
40	24.008151593028394	26.503128102569462	27.40844896466218	22.080271339739966
41	17.368783579005374	26.85921400781907	28.64220872068443	27.129793692491127
42	21.805986385509545	27.638650711839286	25.06909377657224	25.48626912607892
43	21.720955199490763	27.798072309760535	27.023955992798477	23.457016497950224
44	19.88228643633824	31.120654027580507	28.539981283638383	20.457078252442866
45	18.548864429887274	35.848863004783595	24.2014906584454	21.400781906883726
46	20.361691313043025	33.54760558830655	26.10333901791355	19.987364080736874
47	22.470844753955852	28.332391181458448	27.036021870591085	22.16074219399461
48	22.655253169668093	25.720461163549647	30.493798423835333	21.130487242946923
49	20.853162067540413	26.784633582093097	31.490230914299016	20.871973436067474
50	19.52430039285358	34.1938426020493	26.421422158462025	19.86043484663509
51	19.2312040700961	33.205485699084605	25.382901606091846	22.18040862472745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	796.0
1	1512.5
2	2229.0
3	7238.0
4	12247.0
5	9277.5
6	6308.0
7	6449.5
8	6591.0
9	6915.5
10	7240.0
11	7341.0
12	7442.0
13	7179.0
14	6916.0
15	6639.5
16	6363.0
17	6416.0
18	6469.0
19	6045.0
20	5621.0
21	5459.0
22	5297.0
23	5585.0
24	5873.0
25	6866.0
26	8561.0
27	9263.0
28	11551.5
29	13840.0
30	16670.0
31	19500.0
32	22043.5
33	24587.0
34	26461.0
35	28335.0
36	28673.0
37	29011.0
38	35846.5
39	42682.0
40	56663.0
41	70644.0
42	81084.0
43	91524.0
44	95571.0
45	99618.0
46	94577.5
47	89537.0
48	81980.0
49	74423.0
50	72029.0
51	69635.0
52	64491.0
53	59347.0
54	55550.5
55	51754.0
56	47808.5
57	43863.0
58	43246.5
59	42630.0
60	40829.5
61	39029.0
62	32575.5
63	26122.0
64	20934.0
65	15746.0
66	12648.0
67	9550.0
68	7638.5
69	5727.0
70	4675.0
71	3623.0
72	2951.5
73	2280.0
74	1926.5
75	1191.0
76	809.0
77	580.0
78	351.0
79	250.0
80	149.0
81	100.5
82	52.0
83	52.0
84	52.0
85	43.5
86	35.0
87	20.0
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1052555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.150016266587404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82766807040466	15.095511258054017
2	5.380839425772833	2.0608632506288913
3	2.093704963437638	1.2028345232179665
4	1.2193831360349119	0.9340482756108371
5	0.8438294585987083	0.8079673929195452
6	0.6803994346076719	0.7817796144308273
7	0.574248520350832	0.7697807954047526
8	0.4859237158519385	0.7444357650308174
9	0.4377240150459194	0.7544179807564776
>10	6.4589032029972335	30.431272386022645
>50	2.574662786275995	34.69485248536744
>100	0.41922389220853856	10.401458563903507
>500	0.0014954478913503158	0.2033287452608659
>1k	0.0014954478913503158	0.48693893407308775
>5k	4.984826304501053E-4	0.6305100293183189
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6605	0.6275206521274423	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2245	0.2132905168851034	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1665	0.158186508068462	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1191	0.11315323189762054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007410539116720741	0.0
2	0.0	0.0	0.0	0.024416776320477316	0.0
3	0.0	0.0	0.0	0.03515255734854711	0.0
4	0.0	0.0	0.0	0.05472398116963009	0.0
5	0.0	0.0	0.0	0.09614699469386398	0.0
6	0.0	0.0	0.0	0.15201105880452803	0.0
7	0.0	0.0	0.0	0.18307831894770343	0.0
8	0.0	0.0	0.0	0.26810950496648633	0.0
9	0.0	0.0	0.0	0.3037370968737976	0.0
10	0.0	0.0	0.0	0.36957688671850875	0.0
11	0.0	0.0	0.0	0.4260109922996898	0.0
12	0.0	0.0	0.0	0.4741794965583746	0.0
13	0.0	0.0	0.0	0.497456189937818	0.0
14	0.0	0.0	0.0	0.5070518880248538	0.0
15	0.0	0.0	0.0	0.522633021552318	0.0
16	0.0	0.0	0.0	0.5489499361078518	0.0
17	0.0	0.0	0.0	0.5811572791920612	0.0
18	0.0	0.0	0.0	0.6330310530091064	0.0
19	0.0	0.0	0.0	0.6547876358005045	0.0
20	0.0	0.0	0.0	0.6770192531506667	0.0
21	0.0	0.0	0.0	0.7100816584406515	0.0
22	0.0	0.0	0.0	0.7480844231417836	0.0
23	0.0	0.0	0.0	0.7878923191662194	0.0
24	0.0	0.0	0.0	0.8166794134273269	0.0
25	0.0	0.0	0.0	0.8427113072476022	0.0
26	0.0	0.0	0.0	0.8701683047441702	0.0
27	0.0	0.0	0.0	0.8966752331232097	0.0
28	0.0	0.0	0.0	0.926697417237104	0.0
29	0.0	0.0	0.0	0.959664815615336	0.0
30	0.0	0.0	0.0	1.003272988109885	0.0
31	0.0	0.0	0.0	1.0431758910460736	0.0
32	0.0	0.0	0.0	1.082033717952981	0.0
33	0.0	0.0	0.0	1.1226016692714396	0.0
34	0.0	0.0	0.0	1.1646897311779432	0.0
35	0.0	0.0	0.0	1.2150433944069432	0.0
36	0.0	0.0	0.0	1.265397057635943	0.0
37	0.0	0.0	0.0	1.3147056448356618	0.0
38	0.0	0.0	0.0	1.3589788657124806	0.0
39	0.0	0.0	0.0	1.4100925842355032	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACC	20	7.033014E-4	45.000004	18
ACGCCTA	20	7.033014E-4	45.000004	38
ACACCGT	20	7.033014E-4	45.000004	19
TTCGTAC	20	7.033014E-4	45.000004	33
CGTTATG	20	7.033014E-4	45.000004	2
GCGATAT	85	0.0	45.000004	9
CGTACAT	20	7.033014E-4	45.000004	35
CTCGACC	20	7.033014E-4	45.000004	28
ATCGGAT	20	7.033014E-4	45.000004	40
TGCGTTA	20	7.033014E-4	45.000004	22
GTCGATG	50	2.1827873E-11	45.0	1
CTAACCG	25	3.890582E-5	45.0	3
CGGATAT	25	3.890582E-5	45.0	34
TACGAAG	35	1.2117926E-7	45.0	1
ACGGATA	25	3.890582E-5	45.0	33
CGGGCTA	45	3.8562575E-10	45.0	6
ACGTAAG	25	3.890582E-5	45.0	1
CGCGTAA	25	3.890582E-5	45.0	31
CGTAAGG	125	0.0	41.399998	2
ACGTAGG	60	3.6379788E-12	41.249996	2
>>END_MODULE