FastQCFastQC Report
Thu 26 May 2016
SRR1545435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545435_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences773190
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.698534642196614No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27570.3565747099677957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT207.031851E-445.00000442
CGTGGTA207.031851E-445.0000043
ACGCCGA207.031851E-445.00000412
CGCGGTA207.031851E-445.00000432
CCGATTC207.031851E-445.00000411
CACGGAA207.031851E-445.00000411
CCGTGTT207.031851E-445.00000423
GCACGGT302.1645756E-645.0000043
TGTATCG207.031851E-445.0000041
TAACGCG207.031851E-445.0000041
CCTAAAC207.031851E-445.00000420
TAGCCGT302.1645756E-645.00000444
ACGATAT302.1645756E-645.00000438
GCCGACC207.031851E-445.00000414
TGCGCAC207.031851E-445.00000412
CATAGTA207.031851E-445.00000431
TACGATA302.1645756E-645.00000437
ACTACGA302.1645756E-645.00000435
ACTCGAA207.031851E-445.00000413
GTTCACG406.8102963E-945.0000041