FastQCFastQC Report
Thu 26 May 2016
SRR1545434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545434_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1145408
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115131.005144018550595No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA26200.2287394535396994No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19630.17137997988489692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCG253.890798E-545.01
GTCACGC207.0332777E-445.022
GTATACG207.0332777E-445.01
CGGTCAT207.0332777E-445.032
CGTTCGA650.041.5384614
CGTTTTT87850.041.132611
TATACGG900.040.02
ATAGGGC4750.039.7894744
GTTACCG356.2482686E-638.5714338
CGTAAGG1400.038.571432
GGGCGAT22200.038.4121637
AGGGCGA11050.037.669686
TAACGGG3050.037.622953
TATCGTG301.1399197E-437.4999961
CATTAGA4150.037.4096417
TAGGGTC3250.037.3846175
CGGTAGT3200.037.26562512
GTTGATC6000.037.12516
TAGTAGG5400.037.0833362
TTGCGGG6050.036.8181843
Produced by FastQC (version 0.11.5)