##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545434_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1145408
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81769378247751	31.0	31.0	33.0	28.0	34.0
2	31.102698776331227	31.0	31.0	34.0	28.0	34.0
3	31.17974817706878	31.0	31.0	34.0	28.0	34.0
4	35.059274075264014	35.0	35.0	37.0	32.0	37.0
5	34.9421350296139	35.0	35.0	37.0	32.0	37.0
6	34.99291868050511	36.0	35.0	37.0	32.0	37.0
7	35.473335265686984	36.0	35.0	37.0	33.0	37.0
8	35.3753553318992	37.0	35.0	37.0	33.0	37.0
9	37.0627156436833	39.0	37.0	39.0	33.0	39.0
10	36.44792772531709	38.0	35.0	39.0	32.0	39.0
11	36.16121242386992	38.0	35.0	39.0	31.0	39.0
12	35.64325986897245	37.0	35.0	39.0	30.0	39.0
13	35.309025255629436	37.0	35.0	39.0	30.0	39.0
14	36.3081530773314	38.0	35.0	40.0	30.0	41.0
15	36.571977845448956	38.0	35.0	40.0	31.0	41.0
16	36.604664014918704	38.0	35.0	40.0	31.0	41.0
17	36.511108705369615	38.0	35.0	40.0	31.0	41.0
18	36.39710216796111	38.0	35.0	40.0	30.0	41.0
19	36.266290265128234	37.0	35.0	40.0	30.0	41.0
20	36.07536353858188	37.0	34.0	40.0	30.0	41.0
21	35.91097670000559	37.0	34.0	40.0	30.0	41.0
22	35.81228522936805	37.0	34.0	40.0	30.0	41.0
23	35.69342452645695	37.0	34.0	40.0	29.0	41.0
24	35.58048049254065	37.0	34.0	40.0	29.0	41.0
25	35.42431517852154	37.0	34.0	40.0	29.0	41.0
26	35.27080394060457	37.0	34.0	40.0	28.0	41.0
27	35.17809461781304	37.0	34.0	40.0	27.0	41.0
28	35.17826660892887	37.0	34.0	40.0	27.0	41.0
29	35.15688296222831	36.0	34.0	40.0	27.0	41.0
30	35.01595152120467	36.0	34.0	40.0	27.0	41.0
31	34.83828557160418	36.0	33.0	40.0	26.0	41.0
32	34.547363908755656	36.0	33.0	40.0	24.0	41.0
33	34.23713995362351	36.0	33.0	40.0	23.0	41.0
34	33.97527518578533	36.0	33.0	40.0	23.0	41.0
35	33.73268564563893	36.0	33.0	40.0	21.0	41.0
36	33.542991667597924	36.0	32.0	40.0	20.0	41.0
37	33.49291431524836	36.0	32.0	40.0	20.0	41.0
38	33.41391189864223	36.0	32.0	40.0	20.0	41.0
39	33.30440856009387	36.0	32.0	40.0	19.0	41.0
40	33.138522692350676	35.0	32.0	40.0	18.0	41.0
41	33.02109728585796	35.0	31.0	40.0	18.0	41.0
42	32.95427306252444	35.0	31.0	40.0	18.0	41.0
43	32.852379239537356	35.0	31.0	40.0	18.0	41.0
44	32.75860741325362	35.0	31.0	39.0	18.0	41.0
45	32.69681545789797	35.0	31.0	39.0	18.0	41.0
46	32.62075522433928	35.0	31.0	39.0	18.0	41.0
47	32.53886388081801	35.0	31.0	39.0	18.0	41.0
48	32.474352370509024	35.0	31.0	39.0	18.0	41.0
49	32.479664014918704	35.0	31.0	39.0	18.0	41.0
50	32.39467770436386	35.0	31.0	39.0	18.0	41.0
51	32.2136147119629	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	0.0
13	9.0
14	27.0
15	71.0
16	190.0
17	610.0
18	1342.0
19	2418.0
20	4015.0
21	5882.0
22	8588.0
23	12102.0
24	16286.0
25	22059.0
26	28043.0
27	31210.0
28	32925.0
29	35061.0
30	39743.0
31	46502.0
32	56192.0
33	68788.0
34	100827.0
35	123829.0
36	92583.0
37	110386.0
38	141341.0
39	164337.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.84391587975638	22.63481658937252	23.38494230876683	19.136325222104265
2	35.236003240766614	24.383713052466895	26.36676188746717	14.013521819299324
3	27.902808431580713	24.93879910040789	33.27364572274683	13.88474674526457
4	25.858384086718445	27.08100519640163	31.746766217801863	15.313844499078058
5	21.839903335754595	30.823165195284126	32.50806699446835	14.828864474492931
6	20.662680896239593	38.75928926635749	30.24380831983014	10.334221517572779
7	81.0068551991954	5.0125370173772135	11.51816645247807	2.462441330949321
8	81.78098983069788	4.42855296977147	11.647639967592333	2.142817231938314
9	76.86422654634855	5.790163854277253	13.486373414538749	3.8592361848354475
10	43.43116164720344	24.43260392803263	19.472537296753643	12.663697128010282
11	35.59072400402302	24.04304841593563	26.09725093591105	14.268976644130301
12	31.244499776498852	22.089159496004918	30.30064396267531	16.36569676482092
13	22.092913616807287	30.436839973179865	30.027640805721628	17.442605604291224
14	18.48433047438118	31.829618790858806	32.05870746493826	17.627343269821758
15	17.359927641504164	22.94221796949209	41.88036053528524	17.8174938537185
16	19.4904348494161	20.376843884449908	40.417301083980554	19.715420182153434
17	19.91857923115606	20.298793093814606	31.712891825445606	28.069735849583726
18	23.09063669888808	22.414021903112253	34.37779376431804	20.117547633681625
19	28.228718500307316	22.421268229312176	29.279086578756214	20.070926691624294
20	28.97587584511371	22.173408951220875	29.47124518075655	19.37947002290887
21	24.352021288484103	25.633922584790746	30.932122003687766	19.08193412303738
22	24.89732916131195	23.136209979326143	29.5208344974018	22.445626361960105
23	21.59021064982958	27.393557579482597	29.586400653740853	21.429831116946975
24	20.465807816952562	23.86154104039783	37.24681510867743	18.425836033972175
25	19.797399703860982	25.308274431468963	34.61552564675644	20.278800217913616
26	19.472013465944013	27.349817706878248	31.377640107280552	21.800528719897187
27	20.061934262725597	27.341436413924118	32.09790747052579	20.498721852824495
28	18.567881488517628	25.86405892048947	35.51110172095882	20.056957870034083
29	19.078965748449463	24.322512152874783	35.688767670559315	20.909754428116443
30	19.661814829301	26.85453567637034	33.15630762138906	20.3273418729396
31	26.081186791082306	24.721933145219868	29.36141532100352	19.835464742694306
32	26.398191736045145	24.869915348941163	29.56780465999888	19.164088255014807
33	25.684210342515506	25.411207185561825	27.933452533944237	20.971129937978432
34	20.76631209141197	26.263479912834555	30.359662233893946	22.61054576185953
35	22.021498016427334	25.887020170978374	30.86873847572219	21.222743336872103
36	26.393913784433142	26.978072442308765	26.346769011566185	20.281244761691905
37	21.091087193384368	30.834078337151478	28.06109264122479	20.01374182823937
38	22.44885665195284	30.226434597977313	26.307743616248537	21.016965133821312
39	21.9885839805554	29.493857210705706	27.0465196681008	21.471039140638094
40	24.78811043750349	25.770642426104935	27.56729479801084	21.873952338380732
41	20.324373498351676	25.249954601329833	28.611638822148965	25.814033078169523
42	22.50508115885344	25.903084315807117	26.478948985863553	25.112885539475887
43	22.669040202268537	26.173555623847573	27.555421299659162	23.60198287422473
44	21.53887523048556	27.58073978879142	28.647695842878697	22.232689137844332
45	19.890728893110577	31.030340280493938	26.044518634408004	23.03441219198748
46	21.337636894451585	29.461903531318097	27.345539755266245	21.85491981896407
47	21.38417053137397	27.729158518187408	28.616265994300722	22.270404956137902
48	22.025950578309214	26.61837528636084	30.36210677767224	20.993567357657707
49	22.046554590154773	26.550539196513384	30.28597670000559	21.116929513326255
50	20.586812733977762	30.336962898809855	28.627702966977708	20.448521400234675
51	19.986066100463766	30.655713946471476	26.656789545733922	22.70143040733084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	921.0
1	1612.5
2	2304.0
3	11236.5
4	20169.0
5	14689.5
6	9210.0
7	9224.5
8	9239.0
9	9484.0
10	9729.0
11	9807.5
12	9886.0
13	9785.0
14	9684.0
15	9158.0
16	8632.0
17	8337.0
18	8042.0
19	7787.5
20	7533.0
21	7074.5
22	6616.0
23	6833.0
24	7050.0
25	7896.5
26	9547.0
27	10351.0
28	11959.0
29	13567.0
30	16559.5
31	19552.0
32	20632.0
33	21712.0
34	26016.0
35	30320.0
36	32847.5
37	35375.0
38	38278.0
39	41181.0
40	50368.0
41	59555.0
42	68638.5
43	77722.0
44	79053.0
45	80384.0
46	80099.5
47	79815.0
48	77590.0
49	75365.0
50	73599.0
51	71833.0
52	69644.0
53	67455.0
54	65183.0
55	62911.0
56	60308.5
57	57706.0
58	57015.5
59	56325.0
60	52022.5
61	47720.0
62	43202.5
63	38685.0
64	33421.0
65	28157.0
66	23370.0
67	18583.0
68	15689.5
69	12796.0
70	10440.0
71	8084.0
72	6730.5
73	5377.0
74	4303.5
75	2587.0
76	1944.0
77	1540.5
78	1137.0
79	788.0
80	439.0
81	322.0
82	205.0
83	150.0
84	95.0
85	73.0
86	51.0
87	30.5
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1145408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.491522586726166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35500349157138	19.209137735282212
2	6.069496101106457	3.094413939028033
3	2.812778596336012	2.1510602735987807
4	1.8310646188243846	1.867065003540678
5	1.3887023776987781	1.7700069013674369
6	1.0947121236669912	1.674352729585204
7	0.9583204857538864	1.710033381554231
8	0.792085697168062	1.6153176351985914
9	0.6757704142912507	1.5503775101412043
>10	8.099665415275634	47.82463926585239
>50	0.8720942136129786	13.973211171143326
>100	0.0482390757340833	1.9646892675605216
>500	6.891296533432219E-4	0.09257790093022057
>1k	0.0010336944800148327	0.4918749954546824
>5k	0.0	0.0
>10k+	3.4456482667161094E-4	1.011242289762457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11513	1.005144018550595	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2620	0.2287394535396994	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1963	0.17137997988489692	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011087752137229703	0.0
2	0.0	0.0	0.0	0.03885078504777337	0.0
3	0.0	0.0	0.0	0.05989132256802816	0.0
4	0.0	0.0	0.0	0.08774166061351064	0.0
5	0.0	0.0	0.0	0.16151449963681064	0.0
6	0.0	0.0	0.0	0.26235193049114375	0.0
7	0.0	0.0	0.0	0.33298178465664635	0.0
8	0.0	0.0	0.0	0.4938851483488853	0.0
9	0.0	0.0	0.0	0.5627688998156115	0.0
10	0.0	0.0	0.0	0.6437880650388333	0.0
11	0.0	0.0	0.0	0.6987029949153489	0.0
12	0.0	0.0	0.0	0.7563243839749678	0.0
13	0.0	0.0	0.0	0.7926433201095155	0.0
14	0.0	0.0	0.0	0.8087074649382578	0.0
15	0.0	0.0	0.0	0.8304464435380231	0.0
16	0.0	0.0	0.0	0.8601301894172207	0.0
17	0.0	0.0	0.0	0.8912981225903783	0.0
18	0.0	0.0	0.0	0.9397524724814215	0.0
19	0.0	0.0	0.0	0.9591342124378387	0.0
20	0.0	0.0	0.0	0.9863734145387495	0.0
21	0.0	0.0	0.0	1.0150968039336201	0.0
22	0.0	0.0	0.0	1.0454789908923283	0.0
23	0.0	0.0	0.0	1.085115522154551	0.0
24	0.0	0.0	0.0	1.1169818964072191	0.0
25	0.0	0.0	0.0	1.1544357992959713	0.0
26	0.0	0.0	0.0	1.1842068503101078	0.0
27	0.0	0.0	0.0	1.2177320221266135	0.0
28	0.0	0.0	0.0	1.2472411577359335	0.0
29	0.0	0.0	0.0	1.2830362630608483	0.0
30	0.0	0.0	0.0	1.3376019723976085	0.0
31	0.0	0.0	0.0	1.3748812650164832	0.0
32	8.730513493881655E-5	0.0	0.0	1.4223752584231995	0.0
33	8.730513493881655E-5	0.0	0.0	1.4639325026540762	0.0
34	8.730513493881655E-5	0.0	0.0	1.5138710398390791	0.0
35	8.730513493881655E-5	0.0	0.0	1.5635476616192658	0.0
36	8.730513493881655E-5	0.0	0.0	1.6069383136838575	0.0
37	8.730513493881655E-5	0.0	0.0	1.6552180533050231	0.0
38	8.730513493881655E-5	0.0	0.0	1.7069899983237413	0.0
39	8.730513493881655E-5	0.0	0.0	1.7825089400458178	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGCG	25	3.890798E-5	45.0	1
GTCACGC	20	7.0332777E-4	45.0	22
GTATACG	20	7.0332777E-4	45.0	1
CGGTCAT	20	7.0332777E-4	45.0	32
CGTTCGA	65	0.0	41.53846	14
CGTTTTT	8785	0.0	41.13261	1
TATACGG	90	0.0	40.0	2
ATAGGGC	475	0.0	39.789474	4
GTTACCG	35	6.2482686E-6	38.57143	38
CGTAAGG	140	0.0	38.57143	2
GGGCGAT	2220	0.0	38.412163	7
AGGGCGA	1105	0.0	37.66968	6
TAACGGG	305	0.0	37.62295	3
TATCGTG	30	1.1399197E-4	37.499996	1
CATTAGA	415	0.0	37.40964	17
TAGGGTC	325	0.0	37.384617	5
CGGTAGT	320	0.0	37.265625	12
GTTGATC	600	0.0	37.125	16
TAGTAGG	540	0.0	37.083336	2
TTGCGGG	605	0.0	36.818184	3
>>END_MODULE