FastQCFastQC Report
Thu 26 May 2016
SRR1545433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545433_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1128173
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.6110764927010308No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37590.3331935793535211No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14060.12462627628918614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.0332305E-445.00000416
AACCCGT207.0332305E-445.00000432
CGTGACC207.0332305E-445.00000427
CCGTGTA207.0332305E-445.00000429
CCGTCGA207.0332305E-445.0000045
CCGTAGA207.0332305E-445.0000042
TCCGTAG207.0332305E-445.0000041
TATAGCG406.8157533E-945.0000041
ACCGTTG207.0332305E-445.00000420
ACGTATC207.0332305E-445.00000422
TAAATCG207.0332305E-445.00000417
CCGTTGA207.0332305E-445.00000421
CGACACT253.89076E-545.039
ACGGGTA700.045.05
TCTCGAG253.89076E-545.01
ACTACGC253.89076E-545.035
GTACTAC253.89076E-545.031
CAAATCG253.89076E-545.01
CCACGTG750.045.021
GTGCGAC253.89076E-545.030