##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545433_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1128173
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.960530876026993	31.0	31.0	33.0	30.0	34.0
2	31.237714428549523	31.0	31.0	34.0	28.0	34.0
3	31.344880616713926	31.0	31.0	34.0	28.0	34.0
4	35.150155162373146	37.0	35.0	37.0	32.0	37.0
5	35.02590826052387	37.0	35.0	37.0	32.0	37.0
6	35.07320863023668	37.0	35.0	37.0	32.0	37.0
7	35.58507782051157	37.0	35.0	37.0	35.0	37.0
8	35.5202801343411	37.0	35.0	37.0	35.0	37.0
9	37.23386572803994	39.0	37.0	39.0	34.0	39.0
10	36.64496225312962	39.0	35.0	39.0	32.0	39.0
11	36.26663463848186	38.0	35.0	39.0	32.0	39.0
12	35.322232494484446	37.0	35.0	39.0	30.0	39.0
13	34.776007757675465	37.0	33.0	39.0	27.0	39.0
14	35.72606151716093	38.0	34.0	40.0	27.0	41.0
15	36.15283471595225	38.0	34.0	40.0	30.0	41.0
16	36.29283097539118	38.0	34.0	40.0	31.0	41.0
17	36.21194355830178	37.0	34.0	40.0	31.0	41.0
18	36.15795272533556	37.0	35.0	40.0	31.0	41.0
19	36.076261353533546	37.0	35.0	40.0	30.0	41.0
20	35.9363838702043	36.0	34.0	40.0	30.0	41.0
21	35.74311563917945	36.0	34.0	40.0	30.0	41.0
22	35.61107915186766	36.0	34.0	40.0	30.0	41.0
23	35.593862820684414	36.0	34.0	40.0	30.0	41.0
24	35.497276570171415	36.0	34.0	40.0	29.0	41.0
25	35.370270339743996	35.0	34.0	40.0	29.0	41.0
26	35.23178625973144	35.0	34.0	40.0	29.0	41.0
27	35.103492992652725	35.0	34.0	40.0	29.0	41.0
28	35.173566465426845	36.0	34.0	40.0	29.0	41.0
29	35.25473043584628	36.0	34.0	40.0	29.0	41.0
30	35.185601853616426	36.0	34.0	40.0	29.0	41.0
31	35.01356884094904	36.0	34.0	40.0	29.0	41.0
32	34.77854194347853	35.0	34.0	40.0	27.0	41.0
33	34.53247507252877	35.0	33.0	40.0	26.0	41.0
34	34.38197155932645	35.0	33.0	40.0	25.0	41.0
35	34.19243325270149	35.0	33.0	40.0	24.0	41.0
36	33.968987912314866	35.0	33.0	40.0	23.0	41.0
37	33.927850604472894	35.0	33.0	40.0	23.0	41.0
38	33.94778283117926	35.0	33.0	40.0	23.0	41.0
39	33.90183065895036	35.0	33.0	40.0	23.0	41.0
40	33.7276109249202	35.0	33.0	40.0	23.0	41.0
41	33.719032453356	35.0	33.0	40.0	23.0	41.0
42	33.67976808521388	35.0	33.0	40.0	23.0	41.0
43	33.57663408005687	35.0	33.0	40.0	23.0	41.0
44	33.513852928584534	35.0	33.0	39.0	23.0	41.0
45	33.461393775600015	35.0	32.0	39.0	23.0	41.0
46	33.42305568383572	35.0	32.0	39.0	23.0	41.0
47	33.347202069186196	35.0	32.0	39.0	23.0	41.0
48	33.320072364788025	35.0	32.0	39.0	23.0	41.0
49	33.37750415937981	35.0	32.0	39.0	23.0	41.0
50	33.254940510010435	35.0	32.0	39.0	23.0	41.0
51	33.06659085087128	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	2.0
13	3.0
14	14.0
15	65.0
16	151.0
17	427.0
18	964.0
19	1666.0
20	2843.0
21	4424.0
22	6587.0
23	9142.0
24	12912.0
25	17727.0
26	22993.0
27	26597.0
28	29000.0
29	32338.0
30	37848.0
31	45823.0
32	56463.0
33	71804.0
34	118186.0
35	159520.0
36	78159.0
37	91318.0
38	126019.0
39	175101.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.14764047712541	23.192542278533523	28.252315912541782	16.40750133179929
2	30.91236893632448	25.670442387825272	30.34844833194909	13.068740343901156
3	28.734334184562123	24.992354895924652	32.25214572587715	14.021165193636081
4	25.868550302125648	27.58548555939559	31.816485592191977	14.729478546286783
5	22.503729481205454	32.15721347701106	30.86911315906337	14.469943882720116
6	21.546872687079023	40.16724385355792	27.97301477698899	10.312868682374068
7	85.35942625820685	4.914139941303328	8.031480987401755	1.6949528130880636
8	86.89598137874245	3.8294658709258247	7.610268992432897	1.664283757898833
9	82.8232903996107	5.3112421587823855	9.297244305616248	2.568223135990668
10	51.66450535511841	24.125289295170155	14.256678718600782	9.953526631110654
11	46.50412658342293	20.226419175073325	21.750033017985718	11.519421223518025
12	41.446391643834765	22.237369623275864	24.348127459175146	11.968111273714227
13	21.169536941586088	40.085341521202864	25.04952697857509	13.695594558635953
14	14.868907516843604	42.72128476749577	28.718733740304014	13.691073975356616
15	14.682056741297655	23.105144335132998	49.383294937921754	12.829503985647591
16	15.825675672082207	18.875296607878404	47.748882485221685	17.55014523481771
17	16.064646113672282	18.660258666002466	30.6207469953633	34.65434822496195
18	21.042162859774166	22.858816865853022	36.91428530907937	19.184734965293444
19	30.611173995477643	22.988584197636357	27.315048312625812	19.08519349426019
20	33.2687451304011	22.286741483797254	25.86376380218282	18.580749583618825
21	22.934514476059967	28.60846696384331	28.768105600825404	19.688912959271317
22	22.354107038548165	25.448136057147263	26.020388716978694	26.17736818732588
23	18.80048538654976	31.43507245785886	27.021210399468877	22.74323175612251
24	19.45880640646426	23.465284136386884	41.3165356731636	15.759373783985259
25	16.890317353810097	25.40558939098879	38.245375487624685	19.458717767576427
26	16.911945242440655	35.238123940211295	28.39103577199596	19.458895045352087
27	17.220142655426073	35.447400354378274	29.6213435350784	17.711113455117257
28	14.683829519054258	28.772626184104745	39.88395396805277	16.659590328788227
29	15.644940979796537	24.77084631523711	39.048443811365814	20.53576889360054
30	17.692322010897264	31.191935988540763	33.22717349200876	17.88856850855321
31	28.274830190050636	26.996834705315585	26.95508578914759	17.77324931548619
32	30.640424828461594	25.80012108072078	27.96574638818692	15.593707702630713
33	28.645163463404995	27.72376222441062	25.702972859658935	17.928101452525453
34	19.504278155921124	26.938687594899008	29.890008004091573	23.667026245088298
35	20.52034572711809	25.889380440765734	32.56317958327313	21.02709424884304
36	29.227432317561224	27.351744812187494	25.996190300601064	17.42463256965022
37	20.205766314208905	34.583703031361324	28.54003774243844	16.670492911991335
38	19.972557400327787	34.87098166681883	24.88776100828508	20.268699924568306
39	20.60384355945409	33.25562657500224	27.026440093850855	19.114089771692814
40	24.468233152184993	26.924859928397503	26.2056439925437	22.401262926873805
41	16.743619994451205	25.641812027056133	28.012459082073406	29.602108896419253
42	21.08240491484905	27.12598156488411	24.46787859663367	27.323734923633165
43	21.470288687993776	27.164450842202392	26.21317829800926	25.152082171794575
44	18.94000299599441	31.50527445702033	29.123104346585144	20.431618200400116
45	17.063517740630203	37.80430838178187	23.623061356724545	21.509112520863376
46	19.90864876220225	34.78030408456859	26.330979379935528	18.980067773293634
47	21.374381411361558	27.75700180734692	27.462100227536023	23.406516553755495
48	21.982621459652023	24.571674734282777	32.39822261302123	21.047481193043975
49	20.441013922510113	25.284951864651962	33.8344385125331	20.43959570030483
50	18.370054947246565	35.126261663769654	26.718685875304587	19.784997513679194
51	17.584182567744485	35.64089904651148	25.15970511614797	21.615213269596065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	726.0
1	1309.5
2	1893.0
3	6964.5
4	12036.0
5	8890.5
6	5745.0
7	6092.0
8	6439.0
9	6815.0
10	7191.0
11	7345.0
12	7499.0
13	7248.0
14	6997.0
15	6423.0
16	5849.0
17	5673.0
18	5497.0
19	5599.5
20	5702.0
21	5937.5
22	6173.0
23	6600.0
24	7027.0
25	6863.0
26	7934.0
27	9169.0
28	12175.0
29	15181.0
30	18258.0
31	21335.0
32	23547.0
33	25759.0
34	28275.5
35	30792.0
36	32733.5
37	34675.0
38	41710.5
39	48746.0
40	66634.0
41	84522.0
42	101467.5
43	118413.0
44	119949.5
45	121486.0
46	110746.0
47	100006.0
48	91291.5
49	82577.0
50	76790.0
51	71003.0
52	67131.0
53	63259.0
54	56865.0
55	50471.0
56	47039.5
57	43608.0
58	41689.5
59	39771.0
60	35392.5
61	31014.0
62	26691.5
63	22369.0
64	17308.0
65	12247.0
66	9339.0
67	6431.0
68	5204.0
69	3977.0
70	3152.5
71	2328.0
72	2028.5
73	1729.0
74	1298.5
75	684.5
76	501.0
77	371.0
78	241.0
79	178.5
80	116.0
81	90.0
82	64.0
83	41.0
84	18.0
85	13.0
86	8.0
87	8.0
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1128173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.963049816380522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06880958679318	14.562430651622327
2	5.383585461927471	1.934112276866902
3	1.8826058342192393	1.0145202715406643
4	1.0185376490626246	0.7318417011988412
5	0.6912087963542973	0.620810902121633
6	0.5316581621897765	0.5730121231620161
7	0.42150491458281103	0.5300059644950176
8	0.35272899255009965	0.5068870771887324
9	0.3110846322311641	0.5029225871290934
>10	4.906448601201636	21.733130888032502
>50	2.3698660806927183	30.927223546225964
>100	1.0575075828856928	24.979420720585555
>500	0.002969136872865773	0.3117416345520052
>1k	9.897122909552575E-4	0.4591233368865432
>5k	4.948561454776288E-4	0.6128163183922225
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6894	0.6110764927010308	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3759	0.3331935793535211	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1406	0.12462627628918614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006204722148110263	0.0
2	0.0	0.0	0.0	0.025971194134232958	0.0
3	0.0	0.0	0.0	0.03696241622517114	0.0
4	0.0	0.0	0.0	0.06080627705148058	0.0
5	0.0	0.0	0.0	0.10512572096655388	0.0
6	0.0	0.0	0.0	0.162031886953508	0.0
7	0.0	0.0	0.0	0.19970341428132032	0.0
8	0.0	0.0	0.0	0.29853577421193384	0.0
9	0.0	0.0	0.0	0.3425893014635167	0.0
10	0.0	0.0	0.0	0.4061433840377318	0.0
11	0.0	0.0	0.0	0.4669496610892124	0.0
12	0.0	0.0	0.0	0.5252740492814488	0.0
13	0.0	0.0	0.0	0.5450405212675715	0.0
14	0.0	0.0	0.0	0.554347604489737	0.0
15	0.0	0.0	0.0	0.5668456876737876	0.0
16	0.0	0.0	0.0	0.5964510762090566	0.0
17	0.0	0.0	0.0	0.6301338535845123	0.0
18	0.0	0.0	0.0	0.68313990850694	0.0
19	0.0	0.0	0.0	0.7084019915385318	0.0
20	8.863888783014661E-5	0.0	0.0	0.7329549634674823	0.0
21	8.863888783014661E-5	0.0	0.0	0.7662831852916175	0.0
22	8.863888783014661E-5	0.0	0.0	0.7989022960131115	0.0
23	8.863888783014661E-5	0.0	0.0	0.8417148788350722	0.0
24	8.863888783014661E-5	0.0	0.0	0.8726498506877934	0.0
25	8.863888783014661E-5	0.0	0.0	0.898355128158536	0.0
26	8.863888783014661E-5	0.0	0.0	0.9246808778440895	0.0
27	8.863888783014661E-5	0.0	0.0	0.9510952664174732	0.0
28	8.863888783014661E-5	0.0	0.0	0.9809665716162326	0.0
29	8.863888783014661E-5	0.0	0.0	1.0105719601515015	0.0
30	8.863888783014661E-5	0.0	0.0	1.0542709318517638	0.0
31	8.863888783014661E-5	0.0	0.0	1.0937152369361791	0.0
32	8.863888783014661E-5	0.0	0.0	1.1297026253952187	0.0
33	8.863888783014661E-5	0.0	0.0	1.1658672916299184	0.0
34	8.863888783014661E-5	0.0	0.0	1.2045138467238623	0.0
35	8.863888783014661E-5	0.0	0.0	1.2626609571404386	0.0
36	8.863888783014661E-5	0.0	0.0	1.3103486787930574	0.0
37	8.863888783014661E-5	0.0	0.0	1.3518316782975661	0.0
38	8.863888783014661E-5	0.0	0.0	1.398278455520563	0.0
39	1.7727777566029323E-4	0.0	0.0	1.4481821493689355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	7.0332305E-4	45.000004	16
AACCCGT	20	7.0332305E-4	45.000004	32
CGTGACC	20	7.0332305E-4	45.000004	27
CCGTGTA	20	7.0332305E-4	45.000004	29
CCGTCGA	20	7.0332305E-4	45.000004	5
CCGTAGA	20	7.0332305E-4	45.000004	2
TCCGTAG	20	7.0332305E-4	45.000004	1
TATAGCG	40	6.8157533E-9	45.000004	1
ACCGTTG	20	7.0332305E-4	45.000004	20
ACGTATC	20	7.0332305E-4	45.000004	22
TAAATCG	20	7.0332305E-4	45.000004	17
CCGTTGA	20	7.0332305E-4	45.000004	21
CGACACT	25	3.89076E-5	45.0	39
ACGGGTA	70	0.0	45.0	5
TCTCGAG	25	3.89076E-5	45.0	1
ACTACGC	25	3.89076E-5	45.0	35
GTACTAC	25	3.89076E-5	45.0	31
CAAATCG	25	3.89076E-5	45.0	1
CCACGTG	75	0.0	45.0	21
GTGCGAC	25	3.89076E-5	45.0	30
>>END_MODULE