FastQCFastQC Report
Thu 26 May 2016
SRR1545431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545431_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1655465
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72270.4365540799714884No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC71440.431540382913562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG650.045.0000041
GCGAATC351.2123564E-745.00000432
ATCGTAC351.2123564E-745.00000428
CGGTGTC351.2123564E-745.00000414
ACGTCGA351.2123564E-745.00000416
TATACGT351.2123564E-745.00000413
TATCGAC351.2123564E-745.00000423
ATCGACG351.2123564E-745.00000424
TCGCCTA207.0341874E-445.033
CTTCGAA253.8915525E-545.036
AGGTAGC207.0341874E-445.045
TCCGCGT253.8915525E-545.031
ACGCCCG253.8915525E-545.040
GTCGACA207.0341874E-445.028
CGACGTG253.8915525E-545.042
ACGCATC253.8915525E-545.029
CTATCGT207.0341874E-445.013
ACGTTAG800.045.01
CGGTTAC253.8915525E-545.034
CCGCTAG253.8915525E-545.01