##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545431_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1655465
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9412038309478	31.0	31.0	33.0	28.0	34.0
2	31.22972155859532	31.0	31.0	34.0	28.0	34.0
3	31.314380551687893	31.0	31.0	34.0	28.0	34.0
4	35.144431322921356	35.0	35.0	37.0	32.0	37.0
5	35.00757732721622	35.0	35.0	37.0	32.0	37.0
6	35.04358473299043	37.0	35.0	37.0	32.0	37.0
7	35.57055993331179	37.0	35.0	37.0	35.0	37.0
8	35.52001643042891	37.0	35.0	37.0	35.0	37.0
9	37.27803849673656	39.0	37.0	39.0	34.0	39.0
10	36.69441576837928	39.0	37.0	39.0	32.0	39.0
11	36.257707653136734	38.0	35.0	39.0	32.0	39.0
12	34.953290465216725	35.0	34.0	39.0	30.0	39.0
13	34.231071028381756	35.0	33.0	39.0	25.0	39.0
14	35.12009918663336	36.0	33.0	40.0	25.0	41.0
15	35.669243988849054	36.0	33.0	40.0	30.0	41.0
16	35.90911737789685	36.0	34.0	40.0	31.0	41.0
17	35.860506262590874	36.0	34.0	40.0	31.0	41.0
18	35.81528815166735	36.0	34.0	40.0	30.0	41.0
19	35.69996043407743	36.0	34.0	40.0	30.0	41.0
20	35.50178107057533	35.0	34.0	40.0	30.0	41.0
21	35.26039209527232	35.0	34.0	40.0	29.0	41.0
22	35.12865629898548	35.0	34.0	40.0	29.0	41.0
23	35.138527845650614	35.0	34.0	40.0	29.0	41.0
24	35.06638557746615	35.0	34.0	40.0	29.0	41.0
25	34.93576789602921	35.0	34.0	39.0	29.0	41.0
26	34.77124070880387	35.0	34.0	39.0	29.0	41.0
27	34.584985487461225	35.0	33.0	39.0	27.0	41.0
28	34.70308946428949	35.0	34.0	39.0	28.0	41.0
29	34.814420721670345	35.0	34.0	39.0	29.0	41.0
30	34.761917044455785	35.0	34.0	39.0	29.0	41.0
31	34.508327267565306	35.0	33.0	39.0	27.0	41.0
32	34.194948851229114	35.0	33.0	39.0	25.0	41.0
33	33.91118567894821	35.0	33.0	39.0	24.0	41.0
34	33.76816906428103	35.0	33.0	39.0	23.0	41.0
35	33.59111850748883	35.0	33.0	39.0	23.0	41.0
36	33.30143011178128	35.0	33.0	39.0	22.0	41.0
37	33.25912961010955	35.0	32.0	39.0	23.0	41.0
38	33.30098914806414	35.0	32.0	39.0	23.0	41.0
39	33.30855681032217	35.0	33.0	39.0	23.0	41.0
40	33.1121032459158	35.0	32.0	39.0	22.0	41.0
41	33.12029792233602	35.0	32.0	39.0	22.0	41.0
42	33.06109642909998	35.0	32.0	39.0	22.0	41.0
43	32.8970319517477	35.0	32.0	39.0	21.0	41.0
44	32.85050363493037	35.0	32.0	38.0	21.0	41.0
45	32.80574521358047	35.0	32.0	38.0	22.0	40.0
46	32.768730839975476	35.0	32.0	38.0	22.0	40.0
47	32.68354571072176	35.0	32.0	38.0	21.0	40.0
48	32.698368736276514	35.0	32.0	38.0	20.0	40.0
49	32.76387963502702	35.0	32.0	38.0	20.0	40.0
50	32.63322631405678	35.0	32.0	38.0	20.0	40.0
51	32.425081774607136	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	1.0
13	6.0
14	25.0
15	66.0
16	262.0
17	697.0
18	1647.0
19	2986.0
20	4955.0
21	7561.0
22	11225.0
23	16027.0
24	21821.0
25	29305.0
26	37923.0
27	42711.0
28	46204.0
29	50790.0
30	59160.0
31	71737.0
32	88530.0
33	113483.0
34	196128.0
35	272199.0
36	102106.0
37	113982.0
38	153977.0
39	209868.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.076202154681614	25.152449613854717	28.220953025282924	16.550395206180742
2	30.61405707761867	25.653819319647347	30.453014711878534	13.27910889085544
3	26.796338188968054	25.771792215480243	33.55770131050792	13.874168285043778
4	25.205546477877817	28.774694723234862	31.649536535052086	14.370222263835236
5	22.963699021120952	32.477279797519124	31.361883217102143	13.197137964257777
6	20.876672113273308	39.97620004047201	28.460281552313095	10.686846293941581
7	85.09433905277369	5.342184824203471	8.01267317641871	1.5508029466041264
8	87.69016560301787	3.657643018728877	7.302177937920766	1.3500134403324746
9	84.76923402186092	4.837009541125907	8.448744008481	1.9450124285321648
10	60.65027046781418	19.045766597300457	11.86222602108773	8.441736913797634
11	54.58049551032489	17.354338509119792	18.39948292473716	9.665683055818155
12	47.10905999220763	20.97211357533986	21.949603283669543	9.96922314878297
13	19.48787802822772	47.978845822774865	22.0439574379404	10.489318711057013
14	13.368751378011618	49.051414557239205	27.28598913296264	10.293844931786538
15	11.126541485322855	21.24714204166201	56.84741145237139	10.778905020643746
16	13.005832198204129	15.649681509424845	53.661901640928676	17.682584651442344
17	13.42921777264998	16.128338563485183	29.41463576698994	41.0278078968749
18	20.11042214725168	21.17900408646513	39.0369473229576	19.673626443325592
19	32.4934081964886	21.693663109760703	25.700211118930333	20.11271757482037
20	35.118833681171154	21.561434400606476	24.83048569435174	18.489246223870634
21	22.210436342659012	30.05638899040451	28.881432105178906	18.851742561757572
22	23.054791252004726	24.970204746098528	24.74114523713881	27.23385876475794
23	17.91828881915353	32.79767316131721	24.827827830851152	24.456210188678106
24	17.486023564376172	22.843732727662623	44.68732350125191	14.982920206709293
25	14.773734268015332	24.441169097504325	41.57375722229102	19.211339412189325
26	14.410815088207846	37.98080901740599	28.405795350551056	19.202580543835115
27	15.784628488068305	38.853554741417064	29.409501257954712	15.952315512559915
28	12.879946117858124	28.49972666290136	43.820920406049055	14.79940681319146
29	12.915585651161457	23.971270911798197	42.9751761589644	20.137967278075948
30	15.90495721745854	33.72375737330599	32.83917207551957	17.532113333715905
31	32.44659355528507	26.401283023198918	24.36668851349923	16.78543490801678
32	34.149800811252426	25.28135599363321	26.581655305306967	13.987187889807394
33	30.326222541702784	27.125852857052244	24.542409534481248	18.00551506676372
34	18.143784374782914	28.16785616125983	28.280875766023446	25.407483697933813
35	19.61279761275533	24.784033489080105	33.2028765331795	22.400292364985063
36	34.111865850380404	23.24241225275074	25.596071194498222	17.049650702370634
37	19.50672469668643	34.79995046708931	28.60114831784423	17.092176518380032
38	18.755153385906677	36.26026524269616	23.720646464890528	21.26393490650663
39	19.116502010009274	34.67164814719732	27.13195386190587	19.079895980887546
40	25.821143908207063	25.296215866841038	23.512668645969562	25.36997157898234
41	15.659890121506645	23.304147173150746	27.253973958978296	33.78198874636432
42	20.303902528896714	24.305376435019767	24.592969346981057	30.79775168910246
43	20.94861564575512	25.50606627141015	25.61811938035537	27.927198702479362
44	18.133152920780564	30.360593549244474	30.3968371424343	21.109416387540662
45	15.366437828646331	41.966094118570915	21.51178067793641	21.155687374846345
46	21.06097078464359	36.34356510104412	24.478016750580654	18.11744736373164
47	21.04544644556061	27.62843068261788	25.84705807733779	25.479064794483726
48	23.043253708172628	22.70884615500781	33.57020535015842	20.67769478666115
49	21.011075438018924	22.404702002156494	35.549830410186864	21.034392149637714
50	18.26640853174184	37.201873793767916	24.836043045307513	19.69567462918274
51	16.87012410410368	37.705720145095185	23.198436693013747	22.225719057787387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1865.0
1	2277.0
2	2689.0
3	8408.5
4	14128.0
5	11077.0
6	8026.0
7	9028.0
8	10030.0
9	10889.0
10	11748.0
11	12180.0
12	12612.0
13	12399.0
14	12186.0
15	11634.0
16	11082.0
17	10606.5
18	10131.0
19	9535.5
20	8940.0
21	8136.5
22	7333.0
23	7872.0
24	8411.0
25	8516.5
26	9190.5
27	9759.0
28	11354.5
29	12950.0
30	14733.5
31	16517.0
32	21542.0
33	26567.0
34	28539.5
35	30512.0
36	36255.5
37	41999.0
38	55587.5
39	69176.0
40	101027.5
41	132879.0
42	166810.5
43	200742.0
44	199006.5
45	197271.0
46	178512.5
47	159754.0
48	145725.0
49	131696.0
50	120038.0
51	108380.0
52	104084.0
53	99788.0
54	87156.0
55	74524.0
56	68693.5
57	62863.0
58	58641.5
59	54420.0
60	47269.0
61	40118.0
62	33211.5
63	26305.0
64	20366.5
65	14428.0
66	10947.5
67	7467.0
68	5689.5
69	3912.0
70	3178.5
71	2445.0
72	1985.0
73	1525.0
74	1163.5
75	643.0
76	484.0
77	349.5
78	215.0
79	145.5
80	76.0
81	53.5
82	31.0
83	20.5
84	10.0
85	16.0
86	22.0
87	20.5
88	19.0
89	12.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1655465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.285727415570335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.86398647820698	13.657860237671555
2	6.123221011352239	1.99442216592351
3	1.9262132366060183	0.9410935114688731
4	0.9219468716891095	0.6005830177586655
5	0.5544181269895311	0.45145512452012815
6	0.37757856522246247	0.36894849547051023
7	0.28595441126611765	0.3259882916611921
8	0.21797600446953505	0.28399182335407913
9	0.17600053171818686	0.2579667016102049
>10	2.4761193474803807	9.882468515915093
>50	1.1108233962195975	13.25780994625901
>100	1.95046861407968	55.64416640404934
>500	0.014174375087976256	1.3782363482854905
>1k	3.730098707362173E-4	0.08200895055471669
>5k	7.460197414724346E-4	0.8730004654976544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7227	0.4365540799714884	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7144	0.431540382913562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012987287559688667	0.0
2	0.0	0.0	0.0	0.046029363351082625	0.0
3	0.0	0.0	0.0	0.06403034796869762	0.0
4	0.0	0.0	0.0	0.10528763821645278	0.0
5	0.0	0.0	0.0	0.16514997296832007	0.0
6	0.0	0.0	0.0	0.27122288903721914	0.0
7	6.040598864971473E-5	0.0	0.0	0.3231720392759738	0.0
8	6.040598864971473E-5	0.0	0.0	0.48167735349282526	0.0
9	6.040598864971473E-5	0.0	0.0	0.5509026164853984	0.0
10	6.040598864971473E-5	0.0	0.0	0.6502704678141791	0.0
11	6.040598864971473E-5	0.0	0.0	0.7374363094357175	0.0
12	6.040598864971473E-5	0.0	0.0	0.8123397353613637	0.0
13	6.040598864971473E-5	0.0	0.0	0.8480396746533452	0.0
14	6.040598864971473E-5	0.0	0.0	0.8631411718157738	0.0
15	6.040598864971473E-5	0.0	0.0	0.8821086522517843	0.0
16	6.040598864971473E-5	0.0	0.0	0.917083719679969	0.0
17	6.040598864971473E-5	0.0	0.0	0.9565288302682328	0.0
18	6.040598864971473E-5	0.0	0.0	1.0281099268181448	0.0
19	6.040598864971473E-5	0.0	0.0	1.0532992240850758	0.0
20	6.040598864971473E-5	0.0	0.0	1.0806631369433966	0.0
21	6.040598864971473E-5	0.0	0.0	1.1154569864056323	0.0
22	6.040598864971473E-5	0.0	0.0	1.1494051520267718	0.0
23	6.040598864971473E-5	0.0	0.0	1.193622335718363	0.0
24	6.040598864971473E-5	0.0	0.0	1.2247314198729662	0.0
25	6.040598864971473E-5	0.0	0.0	1.2507059949923436	0.0
26	6.040598864971473E-5	0.0	0.0	1.278130313839314	0.0
27	6.040598864971473E-5	0.0	0.0	1.304165294947341	0.0
28	6.040598864971473E-5	0.0	0.0	1.330623117975916	0.0
29	6.040598864971473E-5	0.0	0.0	1.3587723086866832	0.0
30	6.040598864971473E-5	0.0	0.0	1.4060097918107601	0.0
31	6.040598864971473E-5	0.0	0.0	1.4463609922287695	0.0
32	6.040598864971473E-5	0.0	0.0	1.4764431745763276	0.0
33	6.040598864971473E-5	0.0	0.0	1.5115994599704614	0.0
34	6.040598864971473E-5	0.0	0.0	1.5534608101047138	0.0
35	6.040598864971473E-5	0.0	0.0	1.6095779735602989	0.0
36	6.040598864971473E-5	0.0	0.0	1.6547616530702853	0.0
37	6.040598864971473E-5	0.0	0.0	1.6994620846710742	0.0
38	6.040598864971473E-5	0.0	0.0	1.7419879006804735	0.0
39	1.2081197729942946E-4	0.0	0.0	1.786748738269912	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	65	0.0	45.000004	1
GCGAATC	35	1.2123564E-7	45.000004	32
ATCGTAC	35	1.2123564E-7	45.000004	28
CGGTGTC	35	1.2123564E-7	45.000004	14
ACGTCGA	35	1.2123564E-7	45.000004	16
TATACGT	35	1.2123564E-7	45.000004	13
TATCGAC	35	1.2123564E-7	45.000004	23
ATCGACG	35	1.2123564E-7	45.000004	24
TCGCCTA	20	7.0341874E-4	45.0	33
CTTCGAA	25	3.8915525E-5	45.0	36
AGGTAGC	20	7.0341874E-4	45.0	45
TCCGCGT	25	3.8915525E-5	45.0	31
ACGCCCG	25	3.8915525E-5	45.0	40
GTCGACA	20	7.0341874E-4	45.0	28
CGACGTG	25	3.8915525E-5	45.0	42
ACGCATC	25	3.8915525E-5	45.0	29
CTATCGT	20	7.0341874E-4	45.0	13
ACGTTAG	80	0.0	45.0	1
CGGTTAC	25	3.8915525E-5	45.0	34
CCGCTAG	25	3.8915525E-5	45.0	1
>>END_MODULE