FastQCFastQC Report
Thu 26 May 2016
SRR1545430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545430_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences974319
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.6186885404061709No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32030.3287424344593506No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10040.10304633287455135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT502.1827873E-1145.00000428
ACTCTCG253.8903658E-545.00000442
ACTTACG253.8903658E-545.00000410
TACGCTT302.1651595E-645.00000434
TACGCAT253.8903658E-545.00000425
GTCACGG302.1651595E-645.0000042
ACTACGC302.1651595E-645.00000432
CATACGC253.8903658E-545.00000423
GCGATCC502.1827873E-1145.0000049
TTAACGG900.045.0000042
TCGTAGA253.8903658E-545.00000412
GTATAAC253.8903658E-545.0000049
ACGTAAG253.8903658E-545.0000041
TCATACG302.1651595E-645.0000041
TAGTGTA302.1651595E-645.00000422
TCGCGCG302.1651595E-645.0000041
GTACGCG253.8903658E-545.00000420
CTTACGA253.8903658E-545.00000411
TGATCGG207.0327584E-445.02
GGTCGGA207.0327584E-445.07
Produced by FastQC (version 0.11.5)