##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545430_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	974319
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.007979932650393	31.0	31.0	33.0	30.0	34.0
2	31.29427425719913	31.0	31.0	34.0	30.0	34.0
3	31.388861348285314	31.0	31.0	34.0	30.0	34.0
4	35.21231034189008	37.0	35.0	37.0	33.0	37.0
5	35.07948423462952	37.0	35.0	37.0	32.0	37.0
6	35.138826195527336	37.0	35.0	37.0	32.0	37.0
7	35.606609334314534	37.0	35.0	37.0	35.0	37.0
8	35.54866835194633	37.0	35.0	37.0	35.0	37.0
9	37.2665266714495	39.0	37.0	39.0	34.0	39.0
10	36.67579714651978	39.0	35.0	39.0	32.0	39.0
11	36.32838628826904	38.0	35.0	39.0	32.0	39.0
12	35.35595426138667	37.0	35.0	39.0	30.0	39.0
13	34.81051380502689	37.0	34.0	39.0	27.0	39.0
14	35.73840908367794	37.0	34.0	40.0	29.0	41.0
15	36.154398097543	37.0	34.0	40.0	30.0	41.0
16	36.3012904397841	37.0	35.0	40.0	31.0	41.0
17	36.239903973955144	37.0	34.0	40.0	31.0	41.0
18	36.179331409938634	37.0	35.0	40.0	31.0	41.0
19	36.0848705608738	37.0	35.0	40.0	31.0	41.0
20	35.9299295200032	36.0	34.0	40.0	31.0	41.0
21	35.75904195648448	36.0	34.0	40.0	30.0	41.0
22	35.65550502453509	36.0	34.0	40.0	30.0	41.0
23	35.61934540946035	36.0	34.0	40.0	30.0	41.0
24	35.50581585702424	35.0	34.0	40.0	30.0	41.0
25	35.34891139349638	35.0	34.0	40.0	29.0	41.0
26	35.21164423561483	35.0	34.0	40.0	29.0	41.0
27	35.101763385503105	35.0	34.0	40.0	29.0	41.0
28	35.184897348814914	36.0	34.0	40.0	29.0	41.0
29	35.2833117284996	36.0	34.0	40.0	29.0	41.0
30	35.209388300956874	36.0	34.0	40.0	29.0	41.0
31	35.021065995839145	35.0	34.0	40.0	29.0	41.0
32	34.776041522335085	35.0	34.0	40.0	27.0	41.0
33	34.54216329559415	35.0	33.0	40.0	26.0	41.0
34	34.401951516905655	35.0	34.0	40.0	25.0	41.0
35	34.21636650829964	35.0	33.0	40.0	24.0	41.0
36	33.988298493614515	35.0	33.0	40.0	23.0	41.0
37	33.93520397323669	35.0	33.0	40.0	23.0	41.0
38	33.957110556193605	35.0	33.0	40.0	23.0	41.0
39	33.9220614603636	35.0	33.0	40.0	23.0	41.0
40	33.7070138219618	35.0	33.0	40.0	23.0	41.0
41	33.70323374582657	35.0	33.0	40.0	23.0	41.0
42	33.67881053330583	35.0	33.0	39.0	23.0	41.0
43	33.55943381992961	35.0	33.0	39.0	23.0	41.0
44	33.478620451823275	35.0	33.0	39.0	23.0	41.0
45	33.42527036832906	35.0	33.0	39.0	23.0	41.0
46	33.357556406064134	35.0	32.0	39.0	23.0	41.0
47	33.29403922124068	35.0	32.0	39.0	23.0	41.0
48	33.29744878217504	35.0	32.0	39.0	23.0	41.0
49	33.324390676975405	35.0	32.0	39.0	23.0	41.0
50	33.198546882489204	35.0	32.0	39.0	23.0	41.0
51	33.02849169522508	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	2.0
13	4.0
14	15.0
15	31.0
16	138.0
17	291.0
18	641.0
19	1381.0
20	2324.0
21	3505.0
22	5174.0
23	7493.0
24	10677.0
25	14702.0
26	18995.0
27	21985.0
28	24021.0
29	27206.0
30	32205.0
31	39447.0
32	49695.0
33	63995.0
34	107555.0
35	146873.0
36	68454.0
37	78835.0
38	105924.0
39	142688.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.38581922347814	23.294116198082968	28.842914897482242	16.47714968095665
2	30.878182607544346	26.854038564371628	29.64901638990926	12.618762438174766
3	29.190747588828707	25.17132479198291	32.50167552926711	13.13625208992127
4	26.2153360449709	27.460205538432486	31.725030508488494	14.599427908108126
5	22.843339809651663	31.168744528229457	31.927530921597548	14.060384740521329
6	21.363126450372004	39.93343042679041	28.542499940984424	10.160943181853172
7	84.866250170632	5.528168905666419	7.974287681960426	1.6312932417411545
8	87.04151309786631	3.81199586583039	7.405993314304658	1.7404977219986473
9	82.70381671711216	5.574047103669332	9.067358842432508	2.6547773367860015
10	53.04258666822673	21.623000269932128	14.784274965386079	10.550138096455063
11	48.3935959372649	19.79875174352548	20.00504968085401	11.80260263835561
12	42.98992424452361	21.395456724132444	23.96083828807608	11.653780743267863
13	21.24293994061493	42.535658239241975	23.20933903577781	13.012062784365286
14	15.0951587724349	44.476295751186214	26.99731812681473	13.431227349564157
15	13.728152689211646	22.433617737106633	49.86785642074105	13.970373152940669
16	14.758718653746875	17.54497243715867	48.504134682788695	19.19217422630576
17	15.869545805839772	18.25059349145403	29.621715269844884	36.25814543286131
18	21.14615439091304	22.73249315675872	36.60967301263754	19.511679439690695
19	30.48016101502691	22.737009131506213	26.792662362121643	19.990167491345236
20	32.412279756424745	22.088761483661923	25.698872751121556	19.80008600879178
21	22.189652464952445	28.30140847094227	28.53469962096603	20.974239443139258
22	22.627702015459004	26.18454530805619	24.796601523730935	26.39115115275387
23	19.07311671023556	30.664289621776852	25.47512672954135	24.787466938446236
24	19.52563790709203	23.47198402166026	41.40071167656589	15.601666394681823
25	16.56326110852811	25.754501349147457	37.98612158851464	19.69611595380979
26	15.614085325237422	35.28043690002966	28.5305941893774	20.574883585355515
27	16.93983182099497	35.373424925512076	28.90747280921341	18.779270444279543
28	14.531996194264918	29.746725661718592	38.895064142236784	16.826214001779704
29	14.781093255904892	24.24318934558394	38.97080935504696	22.004908043464205
30	16.861828620811046	32.01015273231867	32.492438308192696	18.635580338677578
31	29.02314334422299	27.773142061275617	25.222232143681893	17.981482450819495
32	31.020127904721146	25.72483960591962	27.226401209460143	16.028631279899088
33	28.590225583202216	27.265915988500687	25.334926240789724	18.808932187507377
34	19.125153055621414	27.006760619468572	29.098683285453735	24.769403039456275
35	20.20816590870136	25.438896295771713	31.816581632914886	22.53635616261204
36	30.561859103640593	25.727302864872797	25.796171479772024	17.914666551714582
37	20.14904769382512	34.76674477250264	28.441814231273334	16.642393302398904
38	19.64602968842853	34.415935643254414	25.458089188448547	20.4799454798685
39	20.503346439923682	34.19701350379085	26.77162202522993	18.528018031055538
40	24.178323526483624	26.93378657298072	25.728534494349386	23.15935540618627
41	16.982425673726983	25.752345997563424	27.616211938800333	29.64901638990926
42	20.600131989625574	26.462277755026843	24.767966138400258	28.169624116947322
43	21.62269236256298	26.587698690059412	26.592933115334915	25.196675832042686
44	19.17113388941404	30.545437377286085	29.101043908617196	21.182384824682675
45	16.54376030848213	39.02633531728315	22.749325426272094	21.68057894796263
46	20.274673900437126	34.90827952652057	26.2467425966239	18.5703039764184
47	21.562137246630726	28.34985256368807	27.427362085723466	22.66064810395774
48	22.3934871433278	24.69386309822553	31.776143131766904	21.13650662667976
49	20.61809325282582	25.25456241744234	33.189643227731366	20.937701102000474
50	18.56486427956347	35.154092242889654	27.319286599152843	18.961756878394038
51	18.02520529723838	34.67221721017449	24.89071854290022	22.41185894968691
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	702.0
1	1120.5
2	1539.0
3	5766.0
4	9993.0
5	7295.5
6	4598.0
7	4797.0
8	4996.0
9	5428.5
10	5861.0
11	5990.0
12	6119.0
13	5916.0
14	5713.0
15	5443.0
16	5173.0
17	4901.5
18	4630.0
19	4478.5
20	4327.0
21	4451.5
22	4576.0
23	4506.0
24	4436.0
25	5325.5
26	7091.0
27	7967.0
28	10154.0
29	12341.0
30	13686.0
31	15031.0
32	17819.0
33	20607.0
34	23766.0
35	26925.0
36	27443.5
37	27962.0
38	34867.5
39	41773.0
40	57417.0
41	73061.0
42	88109.5
43	103158.0
44	102767.0
45	102376.0
46	95314.0
47	88252.0
48	81077.5
49	73903.0
50	70291.0
51	66679.0
52	60547.0
53	54415.0
54	49887.5
55	45360.0
56	42204.0
57	39048.0
58	35695.5
59	32343.0
60	28932.0
61	25521.0
62	22552.0
63	19583.0
64	16035.0
65	12487.0
66	9805.5
67	7124.0
68	5559.5
69	3995.0
70	3265.0
71	2535.0
72	1922.0
73	1309.0
74	1073.0
75	602.5
76	368.0
77	305.0
78	242.0
79	186.5
80	131.0
81	89.0
82	47.0
83	40.5
84	34.0
85	24.5
86	15.0
87	11.0
88	7.0
89	4.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	974319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.498521737108593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6635180035013	14.921558411794189
2	5.41515288527469	2.003446467160403
3	1.929305008276692	1.0706787189935658
4	1.0902827333506802	0.806744753699269
5	0.7000481082951769	0.6474927574160041
6	0.5709980171081361	0.6337571538792456
7	0.4543833852788348	0.588379465069306
8	0.3893061196384112	0.576127017321644
9	0.3491682924513104	0.5813187523037691
>10	5.121617964202227	23.13348027164668
>50	2.4065388867858184	31.85463569486513
>100	0.9063443589327362	21.906002152367897
>500	0.0016681184520234411	0.2236156503901837
>1k	0.0011120789680156274	0.43272816982129847
>5k	5.560394840078137E-4	0.6200345632714017
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6028	0.6186885404061709	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3203	0.3287424344593506	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1004	0.10304633287455135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012624202135029699	0.0
2	0.0	0.0	0.0	0.04967572222239328	0.0
3	0.0	0.0	0.0	0.06907388647865843	0.0
4	0.0	0.0	0.0	0.10201997497739447	0.0
5	0.0	0.0	0.0	0.1656541646011214	0.0
6	0.0	0.0	0.0	0.26151599219557453	0.0
7	0.0	0.0	0.0	0.3186841270672131	0.0
8	0.0	0.0	0.0	0.4939860559016092	0.0
9	0.0	0.0	0.0	0.5722971634546796	0.0
10	0.0	0.0	0.0	0.6825280016093291	0.0
11	0.0	0.0	0.0	0.772231681820841	0.0
12	0.0	0.0	0.0	0.8577272946540097	0.0
13	1.026357897156886E-4	0.0	0.0	0.8929313705264909	0.0
14	1.026357897156886E-4	0.0	0.0	0.9086346463529912	0.0
15	1.026357897156886E-4	0.0	0.0	0.9309066127212956	0.0
16	1.026357897156886E-4	0.0	0.0	0.9772979896727868	0.0
17	1.026357897156886E-4	0.0	0.0	1.020097113984229	0.0
18	1.026357897156886E-4	0.0	0.0	1.094302789948672	0.0
19	1.026357897156886E-4	0.0	0.0	1.1260172489708196	0.0
20	1.026357897156886E-4	0.0	0.0	1.1580396153621144	0.0
21	1.026357897156886E-4	0.0	0.0	1.2024809123090077	0.0
22	1.026357897156886E-4	0.0	0.0	1.2449721292513027	0.0
23	1.026357897156886E-4	0.0	0.0	1.2964952956885785	0.0
24	1.026357897156886E-4	0.0	0.0	1.3431945800092167	0.0
25	1.026357897156886E-4	0.0	0.0	1.376038032718237	0.0
26	1.026357897156886E-4	0.0	0.0	1.4064182264740808	0.0
27	1.026357897156886E-4	0.0	0.0	1.441314394977415	0.0
28	1.026357897156886E-4	0.0	0.0	1.4775448287470532	0.0
29	1.026357897156886E-4	0.0	0.0	1.5148016204138481	0.0
30	1.026357897156886E-4	0.0	0.0	1.5741251068695161	0.0
31	1.026357897156886E-4	0.0	0.0	1.6253403659376446	0.0
32	1.026357897156886E-4	0.0	0.0	1.6675236755107927	0.0
33	1.026357897156886E-4	0.0	0.0	1.7138124166725681	0.0
34	1.026357897156886E-4	0.0	0.0	1.7611275157315007	0.0
35	1.026357897156886E-4	0.0	0.0	1.830919852738169	0.0
36	1.026357897156886E-4	0.0	0.0	1.8868563581332192	0.0
37	1.026357897156886E-4	0.0	0.0	1.9395085182573675	0.0
38	1.026357897156886E-4	0.0	0.0	1.9956502952318491	0.0
39	1.026357897156886E-4	0.0	0.0	2.0507657143091738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	50	2.1827873E-11	45.000004	28
ACTCTCG	25	3.8903658E-5	45.000004	42
ACTTACG	25	3.8903658E-5	45.000004	10
TACGCTT	30	2.1651595E-6	45.000004	34
TACGCAT	25	3.8903658E-5	45.000004	25
GTCACGG	30	2.1651595E-6	45.000004	2
ACTACGC	30	2.1651595E-6	45.000004	32
CATACGC	25	3.8903658E-5	45.000004	23
GCGATCC	50	2.1827873E-11	45.000004	9
TTAACGG	90	0.0	45.000004	2
TCGTAGA	25	3.8903658E-5	45.000004	12
GTATAAC	25	3.8903658E-5	45.000004	9
ACGTAAG	25	3.8903658E-5	45.000004	1
TCATACG	30	2.1651595E-6	45.000004	1
TAGTGTA	30	2.1651595E-6	45.000004	22
TCGCGCG	30	2.1651595E-6	45.000004	1
GTACGCG	25	3.8903658E-5	45.000004	20
CTTACGA	25	3.8903658E-5	45.000004	11
TGATCGG	20	7.0327584E-4	45.0	2
GGTCGGA	20	7.0327584E-4	45.0	7
>>END_MODULE