FastQCFastQC Report
Thu 26 May 2016
SRR1545429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545429_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1312101
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86050.6558184164176386No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35900.27360698604756795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT207.033652E-445.039
ACTCCGA207.033652E-445.037
AGTACGG850.042.3529432
CGGTCTA1100.040.90909231
ACGGTCT1100.040.90909230
CGTTTTT65000.040.8807681
TCGTAAG501.0822987E-940.4999961
CATACGA3350.040.29850418
AGGGCGA11900.040.273116
CCAAGCG5100.039.26470620
ACATACG3450.039.13043617
CGTAAGG1800.038.752
TCCGTAG356.248967E-638.5714261
TCGCGCA4400.038.35227234
TCTCGCG4550.038.07692332
TACGGGT950.037.8947374
TGATTCG950.037.89473715
ACGTAGG1550.037.741942
CGACGTC301.14001E-437.50000418
ATCCCGT301.14001E-437.50000431