##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545429_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1312101
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91607200970047	31.0	31.0	33.0	28.0	34.0
2	31.210380908177036	31.0	31.0	34.0	28.0	34.0
3	31.303732715697954	31.0	31.0	34.0	28.0	34.0
4	35.145018561833275	35.0	35.0	37.0	32.0	37.0
5	35.004922639339505	35.0	35.0	37.0	32.0	37.0
6	35.048425387984615	37.0	35.0	37.0	32.0	37.0
7	35.547899894901384	37.0	35.0	37.0	35.0	37.0
8	35.471086448375544	37.0	35.0	37.0	35.0	37.0
9	37.156763846685585	39.0	37.0	39.0	34.0	39.0
10	36.55453962766586	39.0	35.0	39.0	32.0	39.0
11	36.24317487754372	38.0	35.0	39.0	31.0	39.0
12	35.479480619251106	37.0	35.0	39.0	30.0	39.0
13	35.04394402565046	37.0	34.0	39.0	29.0	39.0
14	36.011198833016664	38.0	34.0	40.0	29.0	41.0
15	36.369715441113144	38.0	35.0	40.0	30.0	41.0
16	36.459831217261474	38.0	35.0	40.0	31.0	41.0
17	36.37691381989649	38.0	35.0	40.0	31.0	41.0
18	36.30836040823077	38.0	35.0	40.0	31.0	41.0
19	36.22121620210639	37.0	35.0	40.0	30.0	41.0
20	36.07945196292054	37.0	34.0	40.0	30.0	41.0
21	35.917356971757506	37.0	34.0	40.0	30.0	41.0
22	35.81352959871229	37.0	34.0	40.0	30.0	41.0
23	35.74684951844408	37.0	34.0	40.0	30.0	41.0
24	35.642882674428265	37.0	34.0	40.0	29.0	41.0
25	35.486951080747595	36.0	34.0	40.0	29.0	41.0
26	35.32595127966521	36.0	34.0	40.0	29.0	41.0
27	35.20748478966177	36.0	34.0	40.0	28.0	41.0
28	35.22885890644089	36.0	34.0	40.0	28.0	41.0
29	35.29281968385056	36.0	34.0	40.0	29.0	41.0
30	35.19260483758491	36.0	34.0	40.0	29.0	41.0
31	35.02175213645901	36.0	34.0	40.0	27.0	41.0
32	34.82704304013182	36.0	34.0	40.0	26.0	41.0
33	34.61153905072857	36.0	33.0	40.0	25.0	41.0
34	34.45329894573665	36.0	33.0	40.0	24.0	41.0
35	34.280442587880046	36.0	33.0	40.0	24.0	41.0
36	34.092206316434485	36.0	33.0	40.0	23.0	41.0
37	34.0447854242928	36.0	33.0	40.0	23.0	41.0
38	34.01378933481492	35.0	33.0	40.0	23.0	41.0
39	33.95557811479452	35.0	33.0	40.0	23.0	41.0
40	33.786113264146586	35.0	33.0	40.0	23.0	41.0
41	33.74715742157044	35.0	33.0	40.0	23.0	41.0
42	33.68118384179267	35.0	33.0	40.0	23.0	41.0
43	33.57275316458108	35.0	32.0	40.0	23.0	41.0
44	33.465406245403365	35.0	32.0	40.0	23.0	41.0
45	33.4137882678239	35.0	32.0	39.0	23.0	41.0
46	33.37721257738543	35.0	32.0	39.0	23.0	41.0
47	33.29712575480089	35.0	32.0	39.0	23.0	41.0
48	33.26353840138831	35.0	32.0	39.0	22.0	41.0
49	33.278631751671554	35.0	32.0	39.0	22.0	41.0
50	33.20120783384816	35.0	32.0	39.0	22.0	41.0
51	33.00568934860959	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	6.0
13	5.0
14	31.0
15	78.0
16	165.0
17	491.0
18	1109.0
19	2119.0
20	3486.0
21	5318.0
22	7750.0
23	10928.0
24	15295.0
25	20436.0
26	26596.0
27	30988.0
28	33963.0
29	38139.0
30	43698.0
31	52991.0
32	64877.0
33	81426.0
34	128002.0
35	166149.0
36	99614.0
37	117246.0
38	156529.0
39	204592.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.932145467460206	23.619599405838425	27.686359510434027	16.761895616267346
2	32.62363186980271	25.491482744087534	28.68574903913647	13.199136346973289
3	28.366794934231436	25.08709314298213	32.68063967636638	13.865472246420055
4	26.563808731187617	27.47928703659246	30.54650518519535	15.410399047024582
5	22.551541382866105	32.0434936030077	30.647945546874823	14.757019467251379
6	21.243486591352344	39.748997981100544	28.79862144758673	10.208893979960385
7	84.4446425999218	4.965090339844265	8.555972444194463	2.034294616039466
8	85.50934722250803	4.293038417012105	8.181915873854223	2.0156984866256487
9	80.49090733106674	5.7924656714688885	10.07323369161368	3.643393305850693
10	49.578424221915846	22.05051287972496	16.125740320295463	12.245322578063732
11	42.454048888004806	21.62371646694881	22.037785200986814	13.884449444059566
12	36.96552323334865	22.077416296458885	26.989614366576966	13.967446103615499
13	22.182286272169595	36.4151082881577	26.896405078572457	14.50620036110025
14	16.497129413055855	37.492693016772336	30.460307552543593	15.549870017628217
15	15.747110931246908	22.695051676662086	46.448634670654165	15.10920272143684
16	17.650013223067432	19.059813230841225	43.500233594822355	19.789939951268995
17	18.35918119108209	19.804420543845332	30.31527298584484	31.52112527922774
18	22.662051168317074	22.593306460402058	34.44932973909783	20.29531263218304
19	29.386381078895603	23.498648351003467	26.845951645490707	20.269018924610226
20	30.25216808767008	22.813106612981777	27.105230466252216	19.82949483309593
21	23.732776668869242	26.566018926896636	29.493613677605612	20.207590726628514
22	23.597726089683647	24.770120592850702	26.907989552633527	24.724163764832127
23	20.8761368217843	28.284179342901194	27.31611362235072	23.523570212963786
24	20.316804880112123	23.774999028276024	38.224725078328575	17.683471013283274
25	18.993354932280365	25.507868677792334	35.006756339641534	20.492020050285763
26	18.485543414721885	31.050048738626067	29.1658187898645	21.29858905678755
27	19.073836541546726	31.32510378393127	30.426087625876363	19.174972048645643
28	17.263228973989044	27.389354935328914	37.073289327574635	18.27412676310741
29	17.972930437519672	24.35643292703839	36.51571029974065	21.15492633570129
30	19.85876087282915	29.699390519479827	31.823998305008534	18.61785030268249
31	27.345608303019358	26.59406554830764	27.209109664576125	18.85121648409688
32	29.405205849244837	25.458634663032797	27.780178507599647	17.35598098012272
33	27.201183445481714	26.542545124193946	26.79237345295827	19.46389797736607
34	20.764483831656253	26.854868641971922	29.60137977183159	22.779267754540236
35	21.80640057434603	25.76912905332745	30.755559213810525	21.668911158516
36	28.251331261846456	26.333491095578772	26.659761710417108	18.755415932157664
37	21.141284093221483	32.23555198875696	28.045859274552797	18.577304643468757
38	21.298284202206993	31.84274686171263	26.120092889190694	20.738876046889683
39	21.602224219019725	31.374795080561636	27.16490575039574	19.858074950022903
40	23.84907869135074	26.6929146460524	26.64680539074355	22.81120127185331
41	18.866306785834322	25.494988571763912	27.92749948365255	27.711205158749213
42	21.83581904136953	26.401473667042403	25.851744644657693	25.91096264693038
43	22.05264686178884	26.758991876387565	27.098980947350853	24.08938031447274
44	20.32617915846417	29.195694538758833	29.042124043804556	21.436002258972444
45	18.65046974280181	35.20666473084008	24.94152508076741	21.201340445590695
46	21.391645917501776	31.7323132899068	27.1421178704993	19.733922922092127
47	22.4028485612007	26.896100223991905	28.409931857379885	22.291119357427515
48	22.615103562911695	24.587741340034036	31.298276580842483	21.498878516211786
49	21.171159842115813	25.751447487655295	32.21276410886052	20.86462856136837
50	20.075512479603322	32.8960956511732	27.320305372833342	19.70808649639014
51	20.00440514868901	32.39666763457996	25.71250231498947	21.88642490174156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	773.0
1	1635.5
2	2498.0
3	8688.0
4	14878.0
5	10856.5
6	6835.0
7	6982.5
8	7130.0
9	7511.5
10	7893.0
11	7910.0
12	7927.0
13	7649.0
14	7371.0
15	7108.0
16	6845.0
17	6635.5
18	6426.0
19	6197.0
20	5968.0
21	5855.5
22	5743.0
23	6563.5
24	7384.0
25	8053.5
26	9215.0
27	9707.0
28	12817.5
29	15928.0
30	18862.5
31	21797.0
32	25643.5
33	29490.0
34	31452.0
35	33414.0
36	35887.5
37	38361.0
38	46607.5
39	54854.0
40	68633.0
41	82412.0
42	100918.5
43	119425.0
44	117764.5
45	116104.0
46	114008.5
47	111913.0
48	103830.5
49	95748.0
50	93230.0
51	90712.0
52	85813.0
53	80914.0
54	74017.0
55	67120.0
56	65122.5
57	63125.0
58	58428.5
59	53732.0
60	48253.5
61	42775.0
62	38635.5
63	34496.0
64	28184.0
65	21872.0
66	17529.0
67	13186.0
68	10564.0
69	7942.0
70	6344.0
71	4746.0
72	3715.0
73	2684.0
74	2182.0
75	1205.0
76	730.0
77	616.5
78	503.0
79	346.5
80	190.0
81	129.0
82	68.0
83	54.0
84	40.0
85	29.5
86	19.0
87	16.0
88	13.0
89	8.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1312101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.200298287791117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2722417387904	15.22050687326661
2	5.6686003195126835	2.1767763401782316
3	2.1175489013373694	1.2197271163398555
4	1.242317598143244	0.9541147381009
5	0.8282790156428543	0.7951602082930405
6	0.6401161893277999	0.737425306436276
7	0.5125060628555009	0.6888188496788931
8	0.41866780791980857	0.643083743644477
9	0.40787754108442004	0.7048233408340479
>10	5.760692497832552	27.263064358889753
>50	2.5827516530050465	35.092594235539885
>100	0.5460059117774083	13.332023889556208
>500	0.0011973813854767729	0.15916798557580425
>1k	7.98254256984515E-4	0.35328089239502053
>5k	3.991271284922575E-4	0.6594321212709655
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8605	0.6558184164176386	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3590	0.27360698604756795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005487382449979079	0.0
2	0.0	0.0	0.0	0.022787879896440898	0.0
3	0.0	0.0	0.0	0.03521070405403243	0.0
4	0.0	0.0	0.0	0.05685537927339435	0.0
5	0.0	0.0	0.0	0.09709618390657426	0.0
6	0.0	0.0	0.0	0.16187778227438285	0.0
7	0.0	0.0	0.0	0.20364285981033473	0.0
8	0.0	0.0	0.0	0.31613420003490583	0.0
9	0.0	0.0	0.0	0.373218220243716	0.0
10	0.0	0.0	0.0	0.4564435207350654	0.0
11	0.0	0.0	0.0	0.5230542465862003	0.0
12	0.0	0.0	0.0	0.5799858395047333	0.0
13	0.0	0.0	0.0	0.6055936242713023	0.0
14	0.0	0.0	0.0	0.6143581934622411	0.0
15	0.0	0.0	0.0	0.6275431540712186	0.0
16	0.0	0.0	0.0	0.6584858939974895	0.0
17	0.0	0.0	0.0	0.693315529825829	0.0
18	0.0	0.0	0.0	0.7443786720686898	0.0
19	0.0	0.0	0.0	0.7667854837394378	0.0
20	7.621364513859833E-5	0.0	0.0	0.7912500638289278	0.0
21	7.621364513859833E-5	0.0	0.0	0.826689408818376	0.0
22	7.621364513859833E-5	0.0	0.0	0.8608331218404681	0.0
23	7.621364513859833E-5	0.0	0.0	0.9048083950854393	0.0
24	7.621364513859833E-5	0.0	0.0	0.9381899716561454	0.0
25	7.621364513859833E-5	0.0	0.0	0.9659317384865952	0.0
26	7.621364513859833E-5	0.0	0.0	0.9952739918649555	0.0
27	7.621364513859833E-5	0.0	0.0	1.0264453727266423	0.0
28	1.5242729027719666E-4	0.0	0.0	1.0528152939445972	0.0
29	1.5242729027719666E-4	0.0	0.0	1.0877973570632138	0.0
30	1.5242729027719666E-4	0.0	0.0	1.1403847722088467	0.0
31	1.5242729027719666E-4	0.0	0.0	1.182454704325353	0.0
32	1.5242729027719666E-4	0.0	0.0	1.224219781861305	0.0
33	1.5242729027719666E-4	0.0	0.0	1.2654513638812865	0.0
34	1.5242729027719666E-4	0.0	0.0	1.30767372328807	0.0
35	1.5242729027719666E-4	0.0	0.0	1.3605659930142573	0.0
36	1.5242729027719666E-4	0.0	0.0	1.4075898120647725	0.0
37	1.5242729027719666E-4	0.0	0.0	1.4534704264382086	0.0
38	1.5242729027719666E-4	0.0	0.0	1.5039238595199607	0.0
39	1.5242729027719666E-4	0.0	0.0	1.5665714758238884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACT	20	7.033652E-4	45.0	39
ACTCCGA	20	7.033652E-4	45.0	37
AGTACGG	85	0.0	42.352943	2
CGGTCTA	110	0.0	40.909092	31
ACGGTCT	110	0.0	40.909092	30
CGTTTTT	6500	0.0	40.880768	1
TCGTAAG	50	1.0822987E-9	40.499996	1
CATACGA	335	0.0	40.298504	18
AGGGCGA	1190	0.0	40.27311	6
CCAAGCG	510	0.0	39.264706	20
ACATACG	345	0.0	39.130436	17
CGTAAGG	180	0.0	38.75	2
TCCGTAG	35	6.248967E-6	38.571426	1
TCGCGCA	440	0.0	38.352272	34
TCTCGCG	455	0.0	38.076923	32
TACGGGT	95	0.0	37.894737	4
TGATTCG	95	0.0	37.894737	15
ACGTAGG	155	0.0	37.74194	2
CGACGTC	30	1.14001E-4	37.500004	18
ATCCCGT	30	1.14001E-4	37.500004	31
>>END_MODULE