FastQCFastQC Report
Thu 26 May 2016
SRR1545428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545428_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1248146
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117850.9442004380897748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG253.8909995E-545.01
GGCCGTA207.0335204E-445.017
CGCGTAG253.8909995E-545.01
TATAGCG800.042.18751
CGTTTTT80850.041.660481
TCTAGCG501.0822987E-940.51
TCGACAG451.9284926E-840.0000041
ATAGCGG2450.039.4897962
TATTACG403.4585355E-739.3751
CGTTAGG2300.039.1304362
CTAAGCG750.039.0000041
TAGGGAC9300.038.709685
CGATTCA356.2487215E-638.57142610
CGTAAGG1700.038.3823552
AATCGTT2900.038.0172422
CGGGATA1600.037.968756
TAGCCGT950.037.89473744
GGCGATA5700.037.8947378
CTATGCG900.037.5000041
AATACGG900.037.5000042
Produced by FastQC (version 0.11.5)