FastQCFastQC Report
Thu 26 May 2016
SRR1545426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545426_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences942254
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.3623226858150775No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28360.3009804150473227No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10190.10814493756460572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG600.045.0000041
TCGCACC207.0326374E-445.00000445
CACGGTT207.0326374E-445.00000425
CAACGCG207.0326374E-445.00000441
ATTACGC207.0326374E-445.00000423
ATCAAGT302.165083E-645.00000437
CGTTAGA207.0326374E-445.00000416
GAACGTA207.0326374E-445.00000411
CATACGT207.0326374E-445.00000423
GTATACG551.8189894E-1245.0000041
CACAACG302.165083E-645.00000412
ATTGACG207.0326374E-445.0000041
GCGTATG207.0326374E-445.0000041
AATCGAT207.0326374E-445.00000416
CGCTTAA207.0326374E-445.00000427
CGTTGTA253.890269E-545.013
TGATTCG351.2116107E-745.015
ACTAAGC253.890269E-545.024
CCTATGC1400.045.035
TATCGCG253.890269E-545.01