FastQCFastQC Report
Thu 26 May 2016
SRR1545425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545425_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2604341
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC106410.40858704754868885No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92090.3536019284725003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA351.2127202E-745.00000439
TATCGCC351.2127202E-745.00000441
TTCGAAC351.2127202E-745.00000417
TACCCGC207.034933E-445.035
TACGCTA207.034933E-445.015
TTACGAT253.8921717E-545.039
GACGAAC551.8189894E-1245.012
ATCGACT253.8921717E-545.012
TACGTCA253.8921717E-545.034
CTAGACG1150.043.0434761
GCTACGA2150.042.90697510
TACGGGT3100.042.0967754
CACGATA603.6379788E-1241.24999630
AGGATCG603.6379788E-1241.2499961
CGGGTCA1150.041.0869566
TATACGA556.184564E-1140.9090928
ATTGACG1650.040.909091
CGTTTTT83950.040.658131
ACGGGAT4100.040.609765
CGCGAGG2750.040.0909082