##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545425_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2604341
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350245225183645	31.0	31.0	33.0	30.0	34.0
2	31.691104582694816	31.0	31.0	34.0	30.0	34.0
3	31.77431488426439	31.0	31.0	34.0	30.0	34.0
4	35.475351346079485	37.0	35.0	37.0	33.0	37.0
5	35.34000539867859	37.0	35.0	37.0	33.0	37.0
6	35.40832325720787	37.0	35.0	37.0	33.0	37.0
7	35.885278079944214	37.0	35.0	37.0	35.0	37.0
8	35.91896222499281	37.0	35.0	37.0	35.0	37.0
9	37.614868406249414	39.0	37.0	39.0	35.0	39.0
10	36.94967402502207	39.0	37.0	39.0	32.0	39.0
11	36.55845874253794	39.0	35.0	39.0	32.0	39.0
12	35.654874304094584	37.0	35.0	39.0	31.0	39.0
13	35.25028673280496	37.0	35.0	39.0	30.0	39.0
14	36.18342452082888	38.0	35.0	40.0	31.0	41.0
15	36.460812543365094	38.0	35.0	40.0	31.0	41.0
16	36.65418852600332	38.0	35.0	40.0	32.0	41.0
17	36.62882126418929	38.0	35.0	40.0	32.0	41.0
18	36.56454588704014	38.0	35.0	40.0	31.0	41.0
19	36.51247474889041	37.0	35.0	40.0	31.0	41.0
20	36.353088554839786	37.0	35.0	40.0	31.0	41.0
21	36.1554800235453	37.0	35.0	40.0	31.0	41.0
22	36.07257114179748	37.0	35.0	40.0	31.0	41.0
23	36.03238669590503	37.0	34.0	40.0	31.0	41.0
24	35.98624719266793	36.0	34.0	40.0	31.0	41.0
25	35.86789402770221	36.0	34.0	40.0	30.0	41.0
26	35.765497298548844	36.0	34.0	40.0	30.0	41.0
27	35.724794871332136	36.0	34.0	40.0	30.0	41.0
28	35.73872046709705	36.0	34.0	40.0	30.0	41.0
29	35.76249231571442	36.0	34.0	40.0	30.0	41.0
30	35.69240702350422	36.0	34.0	40.0	30.0	41.0
31	35.51925189520113	36.0	34.0	40.0	30.0	41.0
32	35.304717776973135	36.0	34.0	40.0	29.0	41.0
33	35.11645364412725	36.0	34.0	40.0	29.0	41.0
34	34.94192619169302	36.0	34.0	40.0	27.0	41.0
35	34.78321694432488	36.0	34.0	40.0	27.0	41.0
36	34.58947042649177	36.0	33.0	40.0	25.0	41.0
37	34.52624521904006	35.0	33.0	40.0	25.0	41.0
38	34.52215166907867	35.0	33.0	40.0	26.0	41.0
39	34.49729010141145	35.0	33.0	40.0	25.0	41.0
40	34.30674554522622	35.0	33.0	40.0	24.0	41.0
41	34.32390458853123	35.0	33.0	40.0	24.0	41.0
42	34.26473875732863	35.0	33.0	40.0	24.0	41.0
43	34.173134777665446	35.0	33.0	40.0	24.0	41.0
44	34.1144957591959	35.0	33.0	40.0	24.0	41.0
45	34.06202989547068	35.0	33.0	40.0	24.0	41.0
46	34.05592585609949	35.0	33.0	40.0	24.0	41.0
47	34.011864037773854	35.0	33.0	40.0	24.0	41.0
48	34.012115156962935	35.0	33.0	40.0	24.0	41.0
49	33.96414332838902	35.0	33.0	39.0	24.0	41.0
50	33.828057846495525	35.0	33.0	39.0	24.0	41.0
51	33.66035400126174	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	12.0
14	15.0
15	88.0
16	185.0
17	577.0
18	1323.0
19	2639.0
20	4635.0
21	7387.0
22	11297.0
23	16629.0
24	23347.0
25	33027.0
26	42682.0
27	49793.0
28	54602.0
29	63213.0
30	75421.0
31	92170.0
32	115963.0
33	150793.0
34	261411.0
35	417814.0
36	182585.0
37	210397.0
38	294521.0
39	491560.0
40	252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.6746539719645	24.48047317920349	29.773482044018046	14.071390804813962
2	30.568232040274296	25.098134230502072	31.08847881287435	13.24515491634928
3	27.95847394792003	25.454078402175444	32.54685158356759	14.040596066336935
4	25.015195782733517	28.711178758849165	31.247636158245022	15.02598930017229
5	22.175667472116746	33.237544545817926	30.574913193011206	14.01187478905412
6	21.295214413166324	40.32962657347866	27.86136684865768	10.513792164697326
7	86.07187000473441	4.922972836506433	7.1238750993053515	1.8812820594538118
8	87.44384855900206	3.6858460547217127	6.692249594043176	2.1780557922330446
9	82.98694372203947	5.392765386713952	8.464905325377899	3.1553855658686785
10	52.661767410642454	19.319743459093875	16.07270322895504	11.945785901308623
11	47.106465704759856	20.97701491471355	19.290100643502523	12.62641873702407
12	39.96200958323046	22.269817969305862	23.814584956424678	13.953587491038999
13	20.475659677438554	41.99595982246564	24.282035263431325	13.246345236664478
14	15.648373235302135	42.96534132819013	28.029969961690888	13.356315474816855
15	14.033454144445754	22.861483960817726	49.458538647588774	13.646523247147742
16	14.784469468475903	18.48732558447607	47.589812547588814	19.13839239945921
17	16.15030443401997	19.063747796467513	29.905031637562058	34.880916131950464
18	21.164739947648943	23.297832349911168	35.40154687884574	20.135880823594146
19	29.286986611968246	24.733781021763278	26.473338168849626	19.50589419741885
20	31.219222060398387	22.903836325581022	26.23968981020535	19.637251803815246
21	21.89432950600555	28.666061779160255	28.32977709140239	21.109831623431802
22	22.430664801575524	25.634354333783477	24.990621427839134	26.94435943680186
23	18.785788804154294	30.862586734993613	25.167211206213015	25.184413254639082
24	19.216915142832676	23.34932330290081	40.270916903738794	17.162844650527713
25	16.835276179271457	25.273802470567407	37.063810000303334	20.827111349857795
26	16.496418863735588	35.1132973754205	29.079486902828776	19.310796858015138
27	17.667348477023552	35.6850351010102	28.29521940483216	18.352397017134084
28	15.554798699555858	29.686742250726766	38.26538076235024	16.49307828736713
29	15.526154217132088	25.4536560304507	37.143523063992006	21.87666668842521
30	17.9673859913122	32.145444855339605	31.37899376464142	18.50817538870678
31	28.01756759195512	27.799393397408405	25.807795522936512	18.37524348769996
32	29.415272424002847	25.888698906940373	27.70819950229252	16.98782916676426
33	28.295526584268345	27.70105758040134	24.84098664499004	19.162429190340283
34	19.58602963283226	27.790638783477277	28.53501135220004	24.088320231490425
35	20.29292631034108	26.49150015301376	31.422882026585615	21.79269151005955
36	29.973647844118723	25.761948992086676	26.443656955828747	17.820746207965854
37	20.017655138094437	33.49073719608914	28.44312630335275	18.048481362463672
38	19.583648992201866	33.68483620232527	25.344760920324948	21.386753885147915
39	20.987881387268413	32.32994450419511	27.256607333678655	19.425566774857824
40	23.882126034954716	25.883937625679586	26.332496397361176	23.901439942004522
41	17.141303692565604	24.839258760661526	27.980091700741184	30.039345846031683
42	21.184591418712063	25.54358281039234	25.614925234445106	27.65690053645049
43	21.28784210669801	26.23250949088464	27.809607113661382	24.670041288755964
44	19.146187077652275	30.133189163784618	29.448371008251222	21.272252750311882
45	16.719047160106914	38.43636451601384	23.62651434662358	21.218073977255667
46	21.22847968065626	33.37197394657612	26.37949485109669	19.020051521670933
47	20.812213147203074	27.987540802068544	27.56797208967643	23.63227396105195
48	22.313552641531963	24.43397389205177	31.624660518726234	21.627812947690032
49	21.307923962338265	24.69557557938841	32.56063626076616	21.43586419750716
50	19.5085436200559	33.85140425159378	26.57213475501096	20.06791737333936
51	18.68745298714723	33.61717993150666	24.86083811605316	22.834528965292947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1792.0
1	2632.5
2	3473.0
3	9967.0
4	16461.0
5	12613.0
6	8765.0
7	9635.0
8	10505.0
9	11527.0
10	12549.0
11	13131.0
12	13713.0
13	13873.0
14	14033.0
15	13653.5
16	13274.0
17	13415.0
18	13556.0
19	12223.0
20	10890.0
21	10702.5
22	10515.0
23	12206.0
24	13897.0
25	13888.5
26	15967.5
27	18055.0
28	22332.0
29	26609.0
30	34566.0
31	42523.0
32	47429.5
33	52336.0
34	62422.0
35	72508.0
36	77316.0
37	82124.0
38	99894.0
39	117664.0
40	161883.5
41	206103.0
42	244894.0
43	283685.0
44	285578.0
45	287471.0
46	275034.0
47	262597.0
48	240019.0
49	217441.0
50	200922.5
51	184404.0
52	165328.0
53	146252.0
54	128325.0
55	110398.0
56	99025.0
57	87652.0
58	79336.0
59	71020.0
60	63049.5
61	55079.0
62	47505.5
63	39932.0
64	33645.5
65	27359.0
66	23494.0
67	19629.0
68	15771.0
69	11913.0
70	10639.5
71	9366.0
72	7596.0
73	5826.0
74	4784.0
75	3223.5
76	2705.0
77	1962.5
78	1220.0
79	937.5
80	655.0
81	488.5
82	322.0
83	307.5
84	293.0
85	184.5
86	76.0
87	63.5
88	51.0
89	30.0
90	9.0
91	10.5
92	12.0
93	9.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2604341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.122883789218506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.53485608087605	13.618660978650594
2	8.063297406311518	2.7613380889235817
3	2.869747150135022	1.4741504086860888
4	1.4191420979627623	0.9719922089521648
5	0.8862464044384366	0.7587547095906046
6	0.5819973222672344	0.5979283508890924
7	0.4429912908562679	0.5309701875077438
8	0.3645650925471446	0.49939245706323565
9	0.2770598078600536	0.42696566033858135
>10	2.933974088158165	11.530113159949527
>50	1.0561389838496773	13.143926188151841
>100	1.5505235300323994	50.881107100147084
>500	0.018329306059648564	1.8979435607905586
>1k	6.788631873943912E-4	0.13762929032374627
>5k	2.2628772913146374E-4	0.35682098383766314
>10k+	2.2628772913146374E-4	0.4123066661979122
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10641	0.40858704754868885	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9209	0.3536019284725003	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04461781310511949	0.0
2	0.0	0.0	0.0	0.1481372830977203	0.0
3	0.0	0.0	0.0	0.216868681942956	0.0
4	0.0	0.0	0.0	0.3531795567477531	0.0
5	0.0	0.0	0.0	0.5831801595873966	0.0
6	0.0	0.0	0.0	0.9613564429542829	0.0
7	0.0	0.0	0.0	1.190205122908252	0.0
8	0.0	0.0	0.0	1.7862484213856789	0.0
9	0.0	0.0	0.0	2.066280874893111	0.0
10	0.0	0.0	0.0	2.4793220242664074	0.0
11	0.0	0.0	0.0	2.7847735761177206	0.0
12	0.0	0.0	0.0	3.0583552614653766	0.0
13	0.0	0.0	0.0	3.178884792736435	0.0
14	0.0	0.0	0.0	3.2272655539347572	0.0
15	3.839742952247805E-5	0.0	0.0	3.2822890704404686	0.0
16	3.839742952247805E-5	0.0	0.0	3.4061591780799825	0.0
17	3.839742952247805E-5	0.0	0.0	3.550111141359753	0.0
18	3.839742952247805E-5	0.0	0.0	3.7657511055579893	0.0
19	3.839742952247805E-5	0.0	0.0	3.8648548711555053	0.0
20	3.839742952247805E-5	0.0	0.0	3.969564661463303	0.0
21	3.839742952247805E-5	0.0	0.0	4.112095919850741	0.0
22	3.839742952247805E-5	0.0	0.0	4.256163075419079	0.0
23	3.839742952247805E-5	0.0	0.0	4.425649329331297	0.0
24	3.839742952247805E-5	0.0	0.0	4.558581230338116	0.0
25	3.839742952247805E-5	0.0	0.0	4.666554802155325	0.0
26	3.839742952247805E-5	0.0	0.0	4.772301323060229	0.0
27	3.839742952247805E-5	0.0	0.0	4.87209624238915	0.0
28	3.839742952247805E-5	0.0	0.0	4.9827960317024536	0.0
29	3.839742952247805E-5	0.0	0.0	5.103095178396377	0.0
30	3.839742952247805E-5	0.0	0.0	5.259103934546205	0.0
31	3.839742952247805E-5	0.0	0.0	5.40390064127547	0.0
32	3.839742952247805E-5	0.0	0.0	5.531994466162457	0.0
33	7.67948590449561E-5	0.0	0.0	5.655749381513404	0.0
34	7.67948590449561E-5	0.0	0.0	5.790946730862049	0.0
35	7.67948590449561E-5	0.0	0.0	5.9542509986211485	0.0
36	7.67948590449561E-5	0.0	0.0	6.09993084622943	0.0
37	7.67948590449561E-5	0.0	0.0	6.239159925677935	0.0
38	7.67948590449561E-5	0.0	0.0	6.387450798493745	0.0
39	7.67948590449561E-5	0.0	0.0	6.528407762270763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGA	35	1.2127202E-7	45.000004	39
TATCGCC	35	1.2127202E-7	45.000004	41
TTCGAAC	35	1.2127202E-7	45.000004	17
TACCCGC	20	7.034933E-4	45.0	35
TACGCTA	20	7.034933E-4	45.0	15
TTACGAT	25	3.8921717E-5	45.0	39
GACGAAC	55	1.8189894E-12	45.0	12
ATCGACT	25	3.8921717E-5	45.0	12
TACGTCA	25	3.8921717E-5	45.0	34
CTAGACG	115	0.0	43.043476	1
GCTACGA	215	0.0	42.906975	10
TACGGGT	310	0.0	42.096775	4
CACGATA	60	3.6379788E-12	41.249996	30
AGGATCG	60	3.6379788E-12	41.249996	1
CGGGTCA	115	0.0	41.086956	6
TATACGA	55	6.184564E-11	40.909092	8
ATTGACG	165	0.0	40.90909	1
CGTTTTT	8395	0.0	40.65813	1
ACGGGAT	410	0.0	40.60976	5
CGCGAGG	275	0.0	40.090908	2
>>END_MODULE