FastQCFastQC Report
Thu 26 May 2016
SRR1545424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545424_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1324536
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50000.3774906835299305No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29490.22264400514595298No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23650.17855309330965713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG207.033676E-445.0000041
TGTTACG207.033676E-445.0000041
CCACGAA406.8157533E-945.00000426
AATACGC207.033676E-445.00000430
ACGTTAG502.1827873E-1145.01
TCGTCAA253.891129E-545.01
GCGATAT1600.042.1875049
CGCGATT700.041.78571327
GAACGTA700.041.78571320
CGTAAGG2300.041.0869562
GTCGACA501.0822987E-940.521
TGTCGAC501.0822987E-940.520
TAATGCG1000.040.51
CGTTTTT40900.040.2139361
TAACGGG2750.040.0909083
TAGATCG451.9286745E-840.01
ACGTAAG451.9286745E-840.01
ATAACGG850.039.7058832
AACGGGA3300.039.5454564
GCGTAAG1100.038.863641
Produced by FastQC (version 0.11.5)