##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545424_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1324536
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277072876841398	31.0	31.0	33.0	30.0	34.0
2	31.624547011179764	31.0	31.0	34.0	30.0	34.0
3	31.731225123363956	31.0	31.0	34.0	30.0	34.0
4	35.4394459644736	37.0	35.0	37.0	33.0	37.0
5	35.29705043879517	37.0	35.0	37.0	33.0	37.0
6	35.36375002264944	37.0	35.0	37.0	33.0	37.0
7	35.81167216293102	37.0	35.0	37.0	35.0	37.0
8	35.837672211249824	37.0	35.0	37.0	35.0	37.0
9	37.50036465600029	39.0	37.0	39.0	35.0	39.0
10	36.885485181225725	39.0	37.0	39.0	32.0	39.0
11	36.51257270470565	39.0	35.0	39.0	32.0	39.0
12	35.77110550411616	37.0	35.0	39.0	31.0	39.0
13	35.42116031576341	37.0	35.0	39.0	30.0	39.0
14	36.403118526034774	38.0	35.0	41.0	31.0	41.0
15	36.638067217501074	38.0	35.0	41.0	31.0	41.0
16	36.80166261996654	38.0	35.0	41.0	32.0	41.0
17	36.75766759076386	38.0	35.0	40.0	32.0	41.0
18	36.68798960541654	38.0	35.0	40.0	31.0	41.0
19	36.62799954097133	38.0	35.0	40.0	31.0	41.0
20	36.4775385493486	38.0	35.0	40.0	31.0	41.0
21	36.284584186462276	38.0	35.0	40.0	31.0	41.0
22	36.21168016573351	38.0	35.0	40.0	30.0	41.0
23	36.14678649730924	37.0	35.0	40.0	30.0	41.0
24	36.06868820477511	37.0	35.0	40.0	30.0	41.0
25	35.93646982792465	37.0	34.0	40.0	30.0	41.0
26	35.840076071922546	37.0	34.0	40.0	30.0	41.0
27	35.76893266774176	37.0	34.0	40.0	30.0	41.0
28	35.761212983263576	37.0	34.0	40.0	30.0	41.0
29	35.77322096190666	37.0	34.0	40.0	30.0	41.0
30	35.683345715027755	37.0	34.0	40.0	30.0	41.0
31	35.528977694830495	37.0	34.0	40.0	29.0	41.0
32	35.33668243067761	37.0	34.0	40.0	29.0	41.0
33	35.155107146955615	37.0	34.0	40.0	27.0	41.0
34	34.975745468601836	36.0	34.0	40.0	27.0	41.0
35	34.824890376705504	36.0	34.0	40.0	26.0	41.0
36	34.63912419141496	36.0	33.0	40.0	25.0	41.0
37	34.55799540367344	36.0	33.0	40.0	25.0	41.0
38	34.54785751387656	36.0	33.0	40.0	25.0	41.0
39	34.45603592503337	36.0	33.0	40.0	24.0	41.0
40	34.31319873525521	36.0	33.0	40.0	24.0	41.0
41	34.2765617544559	36.0	33.0	40.0	24.0	41.0
42	34.17322594478368	35.0	33.0	40.0	24.0	41.0
43	34.11362243079841	35.0	33.0	40.0	23.0	41.0
44	34.03967351585763	35.0	33.0	40.0	23.0	41.0
45	33.995709440891	35.0	33.0	40.0	23.0	41.0
46	33.9555217827224	35.0	33.0	40.0	23.0	41.0
47	33.89039407007435	35.0	33.0	40.0	23.0	41.0
48	33.84531111272174	35.0	33.0	39.0	23.0	41.0
49	33.782353216522615	35.0	33.0	39.0	24.0	41.0
50	33.64055941099374	35.0	33.0	39.0	24.0	41.0
51	33.463959454480666	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	10.0
15	34.0
16	138.0
17	342.0
18	793.0
19	1521.0
20	2600.0
21	4263.0
22	6241.0
23	9000.0
24	12678.0
25	17002.0
26	22355.0
27	26098.0
28	29018.0
29	33293.0
30	39035.0
31	47356.0
32	59059.0
33	75611.0
34	123792.0
35	185183.0
36	98309.0
37	118396.0
38	161876.0
39	250403.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52927817741458	23.03855840837848	29.62380788442141	14.808355529785524
2	29.69032174285939	26.1616143313583	30.372975894954912	13.775088030827398
3	28.24528740630681	25.416372223933514	32.353292020752924	13.985048349006746
4	26.109067628210937	27.98496983094457	31.190771711754156	14.715190829090337
5	23.663833976577457	31.83537480294986	30.40483610864484	14.095955111827841
6	22.461450651397925	40.316759982363635	26.658241074610277	10.563548291628162
7	85.91816303973619	5.06192357172625	6.954057873851674	2.0658555146858975
8	86.9564889138536	4.016349876485048	6.798909202920872	2.228252006740474
9	81.80683650727501	5.605963144829586	8.995451992244831	3.5917483556505827
10	49.67286657365296	23.097144962462327	15.094493467901213	12.1354949959835
11	42.64927491589508	20.99142643159567	21.165525134839672	15.193773517669584
12	38.07597528492997	20.68256355433148	25.976115409471696	15.26534575126686
13	22.13008932939535	37.02911812136476	24.826278787439527	16.01451376180036
14	16.910903138910534	38.163930614192445	28.02898524464416	16.896181002252863
15	15.82863734923022	22.326006994147384	45.59015383500335	16.255201821619043
16	16.98398533524193	18.56937070793093	43.29040509280231	21.156238864024836
17	17.214934135425537	19.631101004427208	29.269117638176688	33.88484722197056
18	21.16356218328532	22.918893861699495	34.08997565940073	21.827568295614462
19	28.582839575519277	23.656812649863802	25.94433069391847	21.81601708069845
20	31.451617774073338	20.952997880012322	26.590141755301477	21.005242590612863
21	22.862647749853533	27.161889144575913	27.550402556064917	22.425060549505638
22	23.387586294370255	24.80642277748585	24.54255678969843	27.263434138445465
23	21.148236061534	28.719415704820406	24.85647804212192	25.275870191523676
24	20.332856185109353	22.780203784570595	38.19171392850024	18.695226101819806
25	19.33560129735998	24.741494379918706	34.50755585352154	21.41534846919978
26	18.805453381410548	31.43802810946626	27.698303405871943	22.058215103251253
27	19.044329485948285	32.16107376469949	27.907810735231052	20.88678601412117
28	17.14826928071415	27.419186794469912	36.129633320649646	19.30291060416629
29	17.617867691025385	23.18306184203374	34.87311783145192	24.325952635488957
30	19.23571726249796	30.015341221378655	30.563004705043877	20.185936811079504
31	27.958167992413944	27.247806024147327	25.111359751641327	19.6826662317974
32	29.53774000857659	24.71431505070455	26.05591693996992	19.692028000748945
33	28.096631575132726	27.3332699148985	24.57902238972742	19.991076120241352
34	21.067528553395302	25.9951409399216	27.65851588782789	25.278814618855204
35	22.239561627619032	24.40711313244789	30.189515422759367	23.16380981717371
36	30.02432549964667	25.471032874908644	25.056472606256076	19.448169019188608
37	21.565287768697868	31.443312979035674	28.316859639904084	18.674539612362366
38	21.330186570995426	31.358528571514853	25.649963458901837	21.661321398587884
39	21.308292111350692	31.676828715867295	26.525515350281154	20.489363822500863
40	24.56195981083187	25.893142957231813	26.199816388531534	23.345080843404784
41	18.506027771234606	24.79162514269148	27.938387480596976	28.763959605476934
42	21.469254138807855	25.906959116249013	24.657766946311767	27.96601979863137
43	21.81209117758974	25.998613854210078	26.88511297541177	25.304181992788415
44	20.552857755470594	28.88037773227757	28.330826795194696	22.235937717057144
45	18.431737604715913	34.67757765738342	24.03022643401161	22.86045830388906
46	21.869545259622992	32.23430695730429	25.328718887217867	20.56742889585485
47	21.490318118948824	27.83872994014508	27.13131239920999	23.53963954169611
48	22.845132182137746	24.22712557454082	30.359914717304775	22.567827526016657
49	20.973457875059644	25.843842673962808	31.136262057052434	22.046437393925117
50	20.730051882319543	31.390464283341483	27.346104598138517	20.53337923620045
51	19.44794252477849	31.185637838458145	25.465219518382288	23.901200118381077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	799.0
1	1396.5
2	1994.0
3	5597.5
4	9201.0
5	7000.0
6	4799.0
7	5082.5
8	5366.0
9	5551.5
10	5737.0
11	5915.0
12	6093.0
13	5929.0
14	5765.0
15	5781.5
16	5798.0
17	5520.0
18	5242.0
19	5645.0
20	6048.0
21	5817.0
22	5586.0
23	5735.5
24	5885.0
25	6607.0
26	8538.5
27	9748.0
28	11789.5
29	13831.0
30	16982.0
31	20133.0
32	23929.0
33	27725.0
34	33907.0
35	40089.0
36	42870.0
37	45651.0
38	50119.0
39	54587.0
40	69028.5
41	83470.0
42	96623.5
43	109777.0
44	114318.0
45	118859.0
46	114263.5
47	109668.0
48	104888.0
49	100108.0
50	95507.5
51	90907.0
52	84818.5
53	78730.0
54	72328.5
55	65927.0
56	61496.0
57	57065.0
58	53643.0
59	50221.0
60	47878.0
61	45535.0
62	40815.5
63	36096.0
64	32311.0
65	28526.0
66	24562.5
67	20599.0
68	17747.0
69	14895.0
70	12196.0
71	9497.0
72	8539.5
73	7582.0
74	5917.0
75	3401.5
76	2551.0
77	1987.5
78	1424.0
79	1146.5
80	869.0
81	605.5
82	342.0
83	245.0
84	148.0
85	91.5
86	35.0
87	34.0
88	33.0
89	21.5
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1324536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.817487171284025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54858763023297	15.368181405133369
2	6.938752828537289	2.7501729032849687
3	2.845480120133695	1.6917079733067966
4	1.587402904735102	1.2583334680098752
5	1.0294223198015884	1.0200281808250709
6	0.6696524401817527	0.7962497185512556
7	0.5057713381543649	0.7016181903834098
8	0.40348318414064593	0.6396818260428865
9	0.34315798783170415	0.6120476119420593
>10	5.413530840102876	27.734289533387326
>50	1.986754919183166	27.56895672806078
>100	0.7253171272343483	18.88213956616408
>500	0.0015350627031414771	0.19218520718268953
>1k	0.001151297027356108	0.7844076877254689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5000	0.3774906835299305	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2949	0.22264400514595298	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2365	0.17855309330965713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024838886976269427	0.0
2	0.0	0.0	0.0	0.08501090193094034	0.0
3	0.0	0.0	0.0	0.12208048705357952	0.0
4	0.0	0.0	0.0	0.21048880513628923	0.0
5	0.0	0.0	0.0	0.36156057668496744	0.0
6	0.0	0.0	0.0	0.575144805426202	0.0
7	0.0	0.0	0.0	0.7257635881546444	0.0
8	7.549813670598609E-5	0.0	0.0	1.1159379586511804	0.0
9	7.549813670598609E-5	0.0	0.0	1.2852802792827074	0.0
10	1.5099627341197218E-4	0.0	0.0	1.5586590322950828	0.0
11	2.264944101179583E-4	0.0	0.0	1.7555581728242946	0.0
12	2.264944101179583E-4	0.0	0.0	1.9527593059003303	0.0
13	2.264944101179583E-4	0.0	0.0	2.030748881117614	0.0
14	2.264944101179583E-4	0.0	0.0	2.0597401656127126	0.0
15	2.264944101179583E-4	0.0	0.0	2.102396612851595	0.0
16	2.264944101179583E-4	0.0	0.0	2.179706704838525	0.0
17	2.264944101179583E-4	0.0	0.0	2.270228970749002	0.0
18	2.264944101179583E-4	0.0	0.0	2.4085415571943685	0.0
19	2.264944101179583E-4	0.0	0.0	2.469619549789511	0.0
20	2.264944101179583E-4	0.0	0.0	2.535378426860425	0.0
21	2.264944101179583E-4	0.0	0.0	2.627561651778434	0.0
22	3.0199254682394437E-4	0.0	0.0	2.722311813344447	0.0
23	3.774906835299305E-4	0.0	0.0	2.846053259405558	0.0
24	3.774906835299305E-4	0.0	0.0	2.936651023452741	0.0
25	4.529888202359166E-4	0.0	0.0	3.0090537365537817	0.0
26	5.284869569419027E-4	0.0	0.0	3.0774550484094054	0.0
27	5.284869569419027E-4	0.0	0.0	3.145705363991617	0.0
28	5.284869569419027E-4	0.0	0.0	3.214182173983946	0.0
29	5.284869569419027E-4	0.0	0.0	3.300401046102182	0.0
30	6.039850936478887E-4	0.0	0.0	3.4070799132677405	0.0
31	6.039850936478887E-4	0.0	0.0	3.502962546884343	0.0
32	6.039850936478887E-4	0.0	0.0	3.5865389842178694	0.0
33	6.039850936478887E-4	0.0	0.0	3.6737393321132834	0.0
34	6.039850936478887E-4	0.0	0.0	3.763884107340231	0.0
35	6.039850936478887E-4	0.0	0.0	3.8916269546467595	0.0
36	6.039850936478887E-4	0.0	0.0	3.997399844171846	0.0
37	6.039850936478887E-4	0.0	0.0	4.097434875307277	0.0
38	6.039850936478887E-4	0.0	0.0	4.197016917622473	0.0
39	6.039850936478887E-4	0.0	0.0	4.303016301557678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	20	7.033676E-4	45.000004	1
TGTTACG	20	7.033676E-4	45.000004	1
CCACGAA	40	6.8157533E-9	45.000004	26
AATACGC	20	7.033676E-4	45.000004	30
ACGTTAG	50	2.1827873E-11	45.0	1
TCGTCAA	25	3.891129E-5	45.0	1
GCGATAT	160	0.0	42.187504	9
CGCGATT	70	0.0	41.785713	27
GAACGTA	70	0.0	41.785713	20
CGTAAGG	230	0.0	41.086956	2
GTCGACA	50	1.0822987E-9	40.5	21
TGTCGAC	50	1.0822987E-9	40.5	20
TAATGCG	100	0.0	40.5	1
CGTTTTT	4090	0.0	40.213936	1
TAACGGG	275	0.0	40.090908	3
TAGATCG	45	1.9286745E-8	40.0	1
ACGTAAG	45	1.9286745E-8	40.0	1
ATAACGG	85	0.0	39.705883	2
AACGGGA	330	0.0	39.545456	4
GCGTAAG	110	0.0	38.86364	1
>>END_MODULE