FastQCFastQC Report
Thu 26 May 2016
SRR1545423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545423_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5001419
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257500.5148538844675881No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC180380.36065764536024675No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG75370.15069723212552277No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG50770.10151119112395902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1300.045.0000041
CGTGCTA650.045.00000419
TACGCCC207.0355565E-445.00000435
TACGATA207.0355565E-445.00000427
CGCTAAG351.2130295E-745.00000428
GTACGAC351.2130295E-745.00000434
CTTACCG207.0355565E-445.0000041
GGTACGA253.8926883E-545.07
CGAATCG253.8926883E-545.036
TATAGCG6000.042.7500041
TATATCG850.042.352941
AATACGG4550.042.0329672
TACCTAG1500.042.0000041
ACGTAAG1500.042.0000041
GCGTAAT750.042.0000048
AGTAACG2900.041.8965531
GCGAAGT650.041.53846428
ATGCGTC603.6379788E-1241.25000443
CGTTTTT196850.040.8166121
CCACGTA451.9304935E-840.011